Detection of the Endangered Siamese Bat Catfish (<i>Oreoglanis siamensis</i> Smith, 1933) in Doi Inthanon National Park Using Environmental DNA
Siamese bat catfish (<i>Oreoglanis siamensis</i> Smith, 1993) has been listed as an endangered species, and its abundance has been severely declining due to habitat degradation and overfishing. To establish an appropriate management strategy, it is crucial to gain information about the d...
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MDPI AG
2023-02-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/13/3/538 |
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| author | Thanatrinan Rodpai Chatmongkon Suwannapoom Maslin Osathanunkul |
| author_facet | Thanatrinan Rodpai Chatmongkon Suwannapoom Maslin Osathanunkul |
| author_sort | Thanatrinan Rodpai |
| collection | DOAJ |
| description | Siamese bat catfish (<i>Oreoglanis siamensis</i> Smith, 1993) has been listed as an endangered species, and its abundance has been severely declining due to habitat degradation and overfishing. To establish an appropriate management strategy, it is crucial to gain information about the distribution of this endangered species. As <i>O. siamensis</i> live under rocks in streams, detecting their presence is difficult. Recently, environmental DNA (eDNA)–based detection has been demonstrated to be a valid tool for monitoring rare species, such as <i>O. siamensis</i>. Therefore, this study developed an eDNA assay targeting a 160 bp fragment of the <i>COI</i> region to detect the presence of this species in its natural habitat. An amount of 300 mL of water samples (0.7 μm filtered) were collected from 15 sites in the Mae Klang sub-basin, where this fish species was visually detected at two locations. <i>O. siamensis</i> eDNA was detected at 12 of the 15 sites sampled with varying concentrations (0.71–20.27 copies/mL), including at the sites where this species was visually detected previously. The developed <i>O. siamensis</i> eDNA assay was shown to be effective for detecting the presence of this endangered species in the Klang Phat and Klang Rivers within the Doi Inthanon National Park. |
| first_indexed | 2024-03-11T09:54:25Z |
| format | Article |
| id | doaj.art-d011f7ef87ce428ea397391a12fb9aea |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-11T09:54:25Z |
| publishDate | 2023-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-d011f7ef87ce428ea397391a12fb9aea2023-11-16T16:02:15ZengMDPI AGAnimals2076-26152023-02-0113353810.3390/ani13030538Detection of the Endangered Siamese Bat Catfish (<i>Oreoglanis siamensis</i> Smith, 1933) in Doi Inthanon National Park Using Environmental DNAThanatrinan Rodpai0Chatmongkon Suwannapoom1Maslin Osathanunkul2Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, ThailandSchool of Agriculture and Natural Resources, University of Phayao, Phayao 56000, ThailandDepartment of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, ThailandSiamese bat catfish (<i>Oreoglanis siamensis</i> Smith, 1993) has been listed as an endangered species, and its abundance has been severely declining due to habitat degradation and overfishing. To establish an appropriate management strategy, it is crucial to gain information about the distribution of this endangered species. As <i>O. siamensis</i> live under rocks in streams, detecting their presence is difficult. Recently, environmental DNA (eDNA)–based detection has been demonstrated to be a valid tool for monitoring rare species, such as <i>O. siamensis</i>. Therefore, this study developed an eDNA assay targeting a 160 bp fragment of the <i>COI</i> region to detect the presence of this species in its natural habitat. An amount of 300 mL of water samples (0.7 μm filtered) were collected from 15 sites in the Mae Klang sub-basin, where this fish species was visually detected at two locations. <i>O. siamensis</i> eDNA was detected at 12 of the 15 sites sampled with varying concentrations (0.71–20.27 copies/mL), including at the sites where this species was visually detected previously. The developed <i>O. siamensis</i> eDNA assay was shown to be effective for detecting the presence of this endangered species in the Klang Phat and Klang Rivers within the Doi Inthanon National Park.https://www.mdpi.com/2076-2615/13/3/538endemic and endangered fishnondestructive methodsenvironmental DNAconservation planThai national park |
| spellingShingle | Thanatrinan Rodpai Chatmongkon Suwannapoom Maslin Osathanunkul Detection of the Endangered Siamese Bat Catfish (<i>Oreoglanis siamensis</i> Smith, 1933) in Doi Inthanon National Park Using Environmental DNA Animals endemic and endangered fish nondestructive methods environmental DNA conservation plan Thai national park |
| title | Detection of the Endangered Siamese Bat Catfish (<i>Oreoglanis siamensis</i> Smith, 1933) in Doi Inthanon National Park Using Environmental DNA |
| title_full | Detection of the Endangered Siamese Bat Catfish (<i>Oreoglanis siamensis</i> Smith, 1933) in Doi Inthanon National Park Using Environmental DNA |
| title_fullStr | Detection of the Endangered Siamese Bat Catfish (<i>Oreoglanis siamensis</i> Smith, 1933) in Doi Inthanon National Park Using Environmental DNA |
| title_full_unstemmed | Detection of the Endangered Siamese Bat Catfish (<i>Oreoglanis siamensis</i> Smith, 1933) in Doi Inthanon National Park Using Environmental DNA |
| title_short | Detection of the Endangered Siamese Bat Catfish (<i>Oreoglanis siamensis</i> Smith, 1933) in Doi Inthanon National Park Using Environmental DNA |
| title_sort | detection of the endangered siamese bat catfish i oreoglanis siamensis i smith 1933 in doi inthanon national park using environmental dna |
| topic | endemic and endangered fish nondestructive methods environmental DNA conservation plan Thai national park |
| url | https://www.mdpi.com/2076-2615/13/3/538 |
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