Evaluation of clinical, antiinflammatory and antiinfective properties of amniotic membrane used for guided tissue regeneration: A randomized controlled trial
Background: The objective of this study was to evaluate the antiinflammatory, antiinfective and clinical properties of amniotic membrane (AM) when used for guided tissue regeneration (GTR) in contained interdental defects. Materials and Methods: A total of 30 subjects participated in this study. Tw...
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2015-01-01
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| Series: | Dental Research Journal |
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| Online Access: | http://www.drjjournal.net/article.asp?issn=1735-3327;year=2015;volume=12;issue=2;spage=127;epage=135;aulast=Kumar |
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| author | Aravind Kumar Rampalli Viswa Chandra Aileni Amarender Reddy Bavigadda Harish Reddy Chakravarthy Reddy Anumala Naveen |
| author_facet | Aravind Kumar Rampalli Viswa Chandra Aileni Amarender Reddy Bavigadda Harish Reddy Chakravarthy Reddy Anumala Naveen |
| author_sort | Aravind Kumar |
| collection | DOAJ |
| description | Background: The objective of this study was to evaluate the antiinflammatory, antiinfective and clinical properties of amniotic membrane (AM) when used for guided tissue regeneration (GTR) in contained interdental defects.
Materials and Methods: A total of 30 subjects participated in this study. Two sites in each subject were randomly assigned into each of the following experimental groups; test group: AM with bone graft and control group: Bone graft only. Clinical parameters included recording site-specific measures of plaque, gingivitis, probing pocket depth (PPD), and clinical attachment loss (CAL). The levels of interleukin-1β (IL-1β) and human beta-defensin-2 (hBD-2) levels in gingival crevicular fluid (GCF) from the test and control sites were measured by using commercially available enzyme linked immunosorbent assay kits. The evaluation of bone fill was performed by using digital subtraction technique and morphometric area analysis. One-way analysis of variance followed by the post-hoc test was used for intragroup and intergroup comparison. A P < 0.05 was considered as statistically significant.
Results: Combination therapy using an AM increased bone fill and reduced PPD and CAL when compared to controls. AM also resulted in a significant reduction of GCF IL-1β levels and insignificant increase in the hBD-2 levels.
Conclusion: From this trial conducted over a period of 24 weeks, AM demonstrated a marked antiinflammatory effect and its use resulted in an improvement in periodontal parameters. AM has the potential to function as a barrier for GTR and the unique properties associated with this material can augment its potential as a matrix for periodontal regeneration. |
| first_indexed | 2024-12-17T07:05:57Z |
| format | Article |
| id | doaj.art-d014bd38b574472da8a642cf59f0eb23 |
| institution | Directory Open Access Journal |
| issn | 1735-3327 2008-0255 |
| language | English |
| last_indexed | 2024-12-17T07:05:57Z |
| publishDate | 2015-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Dental Research Journal |
| spelling | doaj.art-d014bd38b574472da8a642cf59f0eb232022-12-21T21:59:09ZengWolters Kluwer Medknow PublicationsDental Research Journal1735-33272008-02552015-01-01122127135Evaluation of clinical, antiinflammatory and antiinfective properties of amniotic membrane used for guided tissue regeneration: A randomized controlled trialAravind KumarRampalli Viswa ChandraAileni Amarender ReddyBavigadda Harish ReddyChakravarthy ReddyAnumala NaveenBackground: The objective of this study was to evaluate the antiinflammatory, antiinfective and clinical properties of amniotic membrane (AM) when used for guided tissue regeneration (GTR) in contained interdental defects. Materials and Methods: A total of 30 subjects participated in this study. Two sites in each subject were randomly assigned into each of the following experimental groups; test group: AM with bone graft and control group: Bone graft only. Clinical parameters included recording site-specific measures of plaque, gingivitis, probing pocket depth (PPD), and clinical attachment loss (CAL). The levels of interleukin-1β (IL-1β) and human beta-defensin-2 (hBD-2) levels in gingival crevicular fluid (GCF) from the test and control sites were measured by using commercially available enzyme linked immunosorbent assay kits. The evaluation of bone fill was performed by using digital subtraction technique and morphometric area analysis. One-way analysis of variance followed by the post-hoc test was used for intragroup and intergroup comparison. A P < 0.05 was considered as statistically significant. Results: Combination therapy using an AM increased bone fill and reduced PPD and CAL when compared to controls. AM also resulted in a significant reduction of GCF IL-1β levels and insignificant increase in the hBD-2 levels. Conclusion: From this trial conducted over a period of 24 weeks, AM demonstrated a marked antiinflammatory effect and its use resulted in an improvement in periodontal parameters. AM has the potential to function as a barrier for GTR and the unique properties associated with this material can augment its potential as a matrix for periodontal regeneration.http://www.drjjournal.net/article.asp?issn=1735-3327;year=2015;volume=12;issue=2;spage=127;epage=135;aulast=KumarAmnionguided periodontal tissue regenerationperiodontal bone lossperiodontitis |
| spellingShingle | Aravind Kumar Rampalli Viswa Chandra Aileni Amarender Reddy Bavigadda Harish Reddy Chakravarthy Reddy Anumala Naveen Evaluation of clinical, antiinflammatory and antiinfective properties of amniotic membrane used for guided tissue regeneration: A randomized controlled trial Dental Research Journal Amnion guided periodontal tissue regeneration periodontal bone loss periodontitis |
| title | Evaluation of clinical, antiinflammatory and antiinfective properties of amniotic membrane used for guided tissue regeneration: A randomized controlled trial |
| title_full | Evaluation of clinical, antiinflammatory and antiinfective properties of amniotic membrane used for guided tissue regeneration: A randomized controlled trial |
| title_fullStr | Evaluation of clinical, antiinflammatory and antiinfective properties of amniotic membrane used for guided tissue regeneration: A randomized controlled trial |
| title_full_unstemmed | Evaluation of clinical, antiinflammatory and antiinfective properties of amniotic membrane used for guided tissue regeneration: A randomized controlled trial |
| title_short | Evaluation of clinical, antiinflammatory and antiinfective properties of amniotic membrane used for guided tissue regeneration: A randomized controlled trial |
| title_sort | evaluation of clinical antiinflammatory and antiinfective properties of amniotic membrane used for guided tissue regeneration a randomized controlled trial |
| topic | Amnion guided periodontal tissue regeneration periodontal bone loss periodontitis |
| url | http://www.drjjournal.net/article.asp?issn=1735-3327;year=2015;volume=12;issue=2;spage=127;epage=135;aulast=Kumar |
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