Callogenesis and Morphohistological Characterization of Hancornia speciosa Gomes
Hancornia speciosa Gomes belongs to the family Apocynaceae and is distributed across different regions of Brazil. The objective of this study was to evaluate callus induction and histodifferentiation in five mangaba accessions under different in vitro culture conditions. Five acessions from the Acti...
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| Format: | Article |
| Language: | Spanish |
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Universidad Distrital Francisco Jose de Caldas
2023-07-01
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| Series: | Colombia Forestal |
| Subjects: | |
| Online Access: | https://revistas.udistrital.edu.co/index.php/colfor/article/view/19696 |
| _version_ | 1797783685127208960 |
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| author | Ana da Silva Ledo Caroline de Araujo Machado Annie Carolina Araújo de Oliveira Maria de Fátima Arrigoni-Blank Evaristo Mauro de Castro Ana Veruska Cruz da Silva |
| author_facet | Ana da Silva Ledo Caroline de Araujo Machado Annie Carolina Araújo de Oliveira Maria de Fátima Arrigoni-Blank Evaristo Mauro de Castro Ana Veruska Cruz da Silva |
| author_sort | Ana da Silva Ledo |
| collection | DOAJ |
| description | Hancornia speciosa Gomes belongs to the family Apocynaceae and is distributed across different regions of Brazil. The objective of this study was to evaluate callus induction and histodifferentiation in five mangaba accessions under different in vitro culture conditions. Five acessions from the Active Germplasm Bank of Mangaba (Embrapa, Brazil) were evaluated. In vitro-germinated plant seedlings were used for the excision of different explants (internode and node segments, and foliar section). These segments were inoculated in a culture medium containing different concentrations of 6-Benzylaminopurine (BA) and 2,4-Dichlorophenoxyacetic acid (2,4-D). There was a significant effect of accessions, treatments, and time on the callus mass (g). There was no callus induction in the absence of regulators. After 60 days of in vitro culture, all treatments exhibited a linear positive cellular growth. The highest callus mass was observed in the BI accession, in the presence of 22.62 µM 2,4-D and 11.10 µM BA. |
| first_indexed | 2024-03-13T00:29:23Z |
| format | Article |
| id | doaj.art-d0172e9655884667a2e3f63c7774bf8c |
| institution | Directory Open Access Journal |
| issn | 0120-0739 2256-201X |
| language | Spanish |
| last_indexed | 2024-03-13T00:29:23Z |
| publishDate | 2023-07-01 |
| publisher | Universidad Distrital Francisco Jose de Caldas |
| record_format | Article |
| series | Colombia Forestal |
| spelling | doaj.art-d0172e9655884667a2e3f63c7774bf8c2023-07-10T20:49:19ZspaUniversidad Distrital Francisco Jose de CaldasColombia Forestal0120-07392256-201X2023-07-0126251410.14483/2256201X.1969618502Callogenesis and Morphohistological Characterization of Hancornia speciosa GomesAna da Silva Ledo0https://orcid.org/0000-0002-4353-4788Caroline de Araujo Machado1Annie Carolina Araújo de Oliveira2Maria de Fátima Arrigoni-Blank3https://orcid.org/0000-0002-4907-505XEvaristo Mauro de Castro4Ana Veruska Cruz da Silva5https://orcid.org/0000-0002-4177-9335Embrapa Tabuleiros Costeiros. Aracaju, Sergipe, BrazilUniversidade Federal de Sergipe. São Cristóvão, Sergipe, BrazilUniversidade Federal de Sergipe. São Cristóvão, Sergipe, BrazilUniversidade Federal de Sergipe. São Cristóvão, Sergipe, BrazilUniversidade Federal de Lavras. Lavras, BrazilEmbrapa Tabuleiros Costeiros. Aracaju, Sergipe, BrazilHancornia speciosa Gomes belongs to the family Apocynaceae and is distributed across different regions of Brazil. The objective of this study was to evaluate callus induction and histodifferentiation in five mangaba accessions under different in vitro culture conditions. Five acessions from the Active Germplasm Bank of Mangaba (Embrapa, Brazil) were evaluated. In vitro-germinated plant seedlings were used for the excision of different explants (internode and node segments, and foliar section). These segments were inoculated in a culture medium containing different concentrations of 6-Benzylaminopurine (BA) and 2,4-Dichlorophenoxyacetic acid (2,4-D). There was a significant effect of accessions, treatments, and time on the callus mass (g). There was no callus induction in the absence of regulators. After 60 days of in vitro culture, all treatments exhibited a linear positive cellular growth. The highest callus mass was observed in the BI accession, in the presence of 22.62 µM 2,4-D and 11.10 µM BA.https://revistas.udistrital.edu.co/index.php/colfor/article/view/19696mangabain vitro tissue culturecellular dedifferentiation |
| spellingShingle | Ana da Silva Ledo Caroline de Araujo Machado Annie Carolina Araújo de Oliveira Maria de Fátima Arrigoni-Blank Evaristo Mauro de Castro Ana Veruska Cruz da Silva Callogenesis and Morphohistological Characterization of Hancornia speciosa Gomes Colombia Forestal mangaba in vitro tissue culture cellular dedifferentiation |
| title | Callogenesis and Morphohistological Characterization of Hancornia speciosa Gomes |
| title_full | Callogenesis and Morphohistological Characterization of Hancornia speciosa Gomes |
| title_fullStr | Callogenesis and Morphohistological Characterization of Hancornia speciosa Gomes |
| title_full_unstemmed | Callogenesis and Morphohistological Characterization of Hancornia speciosa Gomes |
| title_short | Callogenesis and Morphohistological Characterization of Hancornia speciosa Gomes |
| title_sort | callogenesis and morphohistological characterization of hancornia speciosa gomes |
| topic | mangaba in vitro tissue culture cellular dedifferentiation |
| url | https://revistas.udistrital.edu.co/index.php/colfor/article/view/19696 |
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