Genome-wide identification, expression analysis, and functional study of the bZIP transcription factor family and its response to hormone treatments in pea (Pisum sativum L.)
Abstract Background Basic leucine zipper (bZIP) protein is a plant-specific transcription factor involved in various biological processes, including light signaling, seed maturation, flower development, cell elongation, seed accumulation protein, and abiotic and biological stress responses. However,...
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| Format: | Article |
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BMC
2023-11-01
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| Series: | BMC Genomics |
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| Online Access: | https://doi.org/10.1186/s12864-023-09793-5 |
| _version_ | 1797453838093910016 |
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| author | Xiaozong Wu Changhe Cheng Rui Ma Jianbo Xu Congcong Ma Yutao Zhu Yanyan Ren |
| author_facet | Xiaozong Wu Changhe Cheng Rui Ma Jianbo Xu Congcong Ma Yutao Zhu Yanyan Ren |
| author_sort | Xiaozong Wu |
| collection | DOAJ |
| description | Abstract Background Basic leucine zipper (bZIP) protein is a plant-specific transcription factor involved in various biological processes, including light signaling, seed maturation, flower development, cell elongation, seed accumulation protein, and abiotic and biological stress responses. However, little is known about the pea bZIP family. Results In this study, we identified 87 bZIP genes in pea, named PsbZIP1 ~ PsbZIP87, via homology analysis using Arabidopsis. The genes were divided into 12 subfamilies and distributed unevenly in 7 pea chromosomes. PsbZIPs in the same subfamily contained similar intron/exon organization and motif composition. 1 tandem repeat event and 12 segmental duplication events regulated the expansion of the PsbZIP gene family. To better understand the evolution of the PsbZIP gene family, we conducted collinearity analysis using Arabidopsis thaliana, Oryza sativa Japonica, Fagopyrum tataricum, Solanum lycopersicum, Vitis vinifera, and Brachypodium distachyon as the related species of pea. In addition, interactions between PsbZIP proteins and promoters containing hormone- and stress-responsive cis-acting elements suggest that the regulation of PsbZIP expression was complex. We also evaluated the expression patterns of bZIP genes in different tissues and at different fruit development stages, all while subjecting them to five hormonal treatments. Conclusion These results provide a deeper understanding of PsbZIP gene family evolution and resources for the molecular breeding of pea. The findings suggested that PsbZIP genes, specifically PSbZIP49, play key roles in the development of peas and their response to various hormones. |
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| format | Article |
| id | doaj.art-d0183ed569df4cb38bd24f9c01a9a9c6 |
| institution | Directory Open Access Journal |
| issn | 1471-2164 |
| language | English |
| last_indexed | 2024-03-09T15:28:39Z |
| publishDate | 2023-11-01 |
| publisher | BMC |
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| series | BMC Genomics |
| spelling | doaj.art-d0183ed569df4cb38bd24f9c01a9a9c62023-11-26T12:25:43ZengBMCBMC Genomics1471-21642023-11-0124111710.1186/s12864-023-09793-5Genome-wide identification, expression analysis, and functional study of the bZIP transcription factor family and its response to hormone treatments in pea (Pisum sativum L.)Xiaozong Wu0Changhe Cheng1Rui Ma2Jianbo Xu3Congcong Ma4Yutao Zhu5Yanyan Ren6Zhengzhou University of Light IndustryChina Tobacco Zhejiang Industrial Co., LTDState Key Laboratory of Crop Stress Biology for Arid Areas, College of Plant Protection, Northwest A&F UniversityZhengzhou University of Light IndustryCollege of Medical Technology, Luoyang PolytechnicCollege of Life Science and Engineering, Henan University of Urban ConstructionState Key Laboratory of Crop Stress Biology for Arid Areas, College of Plant Protection, Northwest A&F UniversityAbstract Background Basic leucine zipper (bZIP) protein is a plant-specific transcription factor involved in various biological processes, including light signaling, seed maturation, flower development, cell elongation, seed accumulation protein, and abiotic and biological stress responses. However, little is known about the pea bZIP family. Results In this study, we identified 87 bZIP genes in pea, named PsbZIP1 ~ PsbZIP87, via homology analysis using Arabidopsis. The genes were divided into 12 subfamilies and distributed unevenly in 7 pea chromosomes. PsbZIPs in the same subfamily contained similar intron/exon organization and motif composition. 1 tandem repeat event and 12 segmental duplication events regulated the expansion of the PsbZIP gene family. To better understand the evolution of the PsbZIP gene family, we conducted collinearity analysis using Arabidopsis thaliana, Oryza sativa Japonica, Fagopyrum tataricum, Solanum lycopersicum, Vitis vinifera, and Brachypodium distachyon as the related species of pea. In addition, interactions between PsbZIP proteins and promoters containing hormone- and stress-responsive cis-acting elements suggest that the regulation of PsbZIP expression was complex. We also evaluated the expression patterns of bZIP genes in different tissues and at different fruit development stages, all while subjecting them to five hormonal treatments. Conclusion These results provide a deeper understanding of PsbZIP gene family evolution and resources for the molecular breeding of pea. The findings suggested that PsbZIP genes, specifically PSbZIP49, play key roles in the development of peas and their response to various hormones.https://doi.org/10.1186/s12864-023-09793-5PeabZIP gene familyEvolutionHormones |
| spellingShingle | Xiaozong Wu Changhe Cheng Rui Ma Jianbo Xu Congcong Ma Yutao Zhu Yanyan Ren Genome-wide identification, expression analysis, and functional study of the bZIP transcription factor family and its response to hormone treatments in pea (Pisum sativum L.) BMC Genomics Pea bZIP gene family Evolution Hormones |
| title | Genome-wide identification, expression analysis, and functional study of the bZIP transcription factor family and its response to hormone treatments in pea (Pisum sativum L.) |
| title_full | Genome-wide identification, expression analysis, and functional study of the bZIP transcription factor family and its response to hormone treatments in pea (Pisum sativum L.) |
| title_fullStr | Genome-wide identification, expression analysis, and functional study of the bZIP transcription factor family and its response to hormone treatments in pea (Pisum sativum L.) |
| title_full_unstemmed | Genome-wide identification, expression analysis, and functional study of the bZIP transcription factor family and its response to hormone treatments in pea (Pisum sativum L.) |
| title_short | Genome-wide identification, expression analysis, and functional study of the bZIP transcription factor family and its response to hormone treatments in pea (Pisum sativum L.) |
| title_sort | genome wide identification expression analysis and functional study of the bzip transcription factor family and its response to hormone treatments in pea pisum sativum l |
| topic | Pea bZIP gene family Evolution Hormones |
| url | https://doi.org/10.1186/s12864-023-09793-5 |
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