High-precision, gelatin-based, hybrid, bilayer scaffolds using melt electro-writing to repair cartilage injury
Articular cartilage injury is a common disease in the field of orthopedics. Because cartilage has poor self-repairing ability, medical intervention is needed. Using melt electro-writing (MEW) technology, tissue engineering scaffolds with high porosity and high precision can be prepared. However, ord...
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| Format: | Article |
| Language: | English |
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KeAi Communications Co., Ltd.
2021-07-01
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| Series: | Bioactive Materials |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2452199X20303443 |
| _version_ | 1797203667179274240 |
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| author | Yu Han Bo Jia Meifei Lian Binbin Sun Qiang Wu Benlin Sun Zhiguang Qiao Kerong Dai |
| author_facet | Yu Han Bo Jia Meifei Lian Binbin Sun Qiang Wu Benlin Sun Zhiguang Qiao Kerong Dai |
| author_sort | Yu Han |
| collection | DOAJ |
| description | Articular cartilage injury is a common disease in the field of orthopedics. Because cartilage has poor self-repairing ability, medical intervention is needed. Using melt electro-writing (MEW) technology, tissue engineering scaffolds with high porosity and high precision can be prepared. However, ordinary materials, especially natural polymer materials, are difficult to print. In this study, gelatin was mixed with poly (lactic-co-glycolic acid) to prepare high-concentration and high-viscosity printer ink, which had good printability and formability. A composite scaffold with full-layer TGF-β1 loading mixed with hydroxyapatite was prepared, and the scaffold was implanted at the cartilage injury site; microfracture surgery was conducted to induce the mesenchyme in the bone marrow. Quality stem cells thereby promoted the repair of damaged cartilage. In summary, this study developed a novel printing method, explored the molding conditions based on MEW printing ink, and constructed a bioactive cartilage repair scaffold. The scaffold can use autologous bone marrow mesenchymal stem cells and induce their differentiation to promote cartilage repair. |
| first_indexed | 2024-04-24T08:22:58Z |
| format | Article |
| id | doaj.art-d01abad3ab15480792efacac7dc98bea |
| institution | Directory Open Access Journal |
| issn | 2452-199X |
| language | English |
| last_indexed | 2024-04-24T08:22:58Z |
| publishDate | 2021-07-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Bioactive Materials |
| spelling | doaj.art-d01abad3ab15480792efacac7dc98bea2024-04-17T00:33:12ZengKeAi Communications Co., Ltd.Bioactive Materials2452-199X2021-07-016721732186High-precision, gelatin-based, hybrid, bilayer scaffolds using melt electro-writing to repair cartilage injuryYu Han0Bo Jia1Meifei Lian2Binbin Sun3Qiang Wu4Benlin Sun5Zhiguang Qiao6Kerong Dai7Department of Orthopedic Surgery, Shanghai Key Laboratory of Orthopedic Implants, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200011, China; Clinical and Translational Research Center for 3D Printing Technology, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200011, ChinaDepartment of Orthopedics, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200080, ChinaClinical and Translational Research Center for 3D Printing Technology, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200011, China; Department of Prosthodontics, Shanghai Ninth People's Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine, National Clinical Research Center for Oral Diseases, Shanghai Key Laboratory of Stomatology & Shanghai Research Institute of Stomatology, Shanghai, 200011, ChinaDepartment of Orthopedic Surgery, Shanghai Key Laboratory of Orthopedic Implants, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200011, China; Clinical and Translational Research Center for 3D Printing Technology, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200011, ChinaDepartment of Orthopedic Surgery, Shanghai Key Laboratory of Orthopedic Implants, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200011, ChinaDepartment of Orthopedic Surgery, Shanghai Key Laboratory of Orthopedic Implants, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200011, China; Clinical and Translational Research Center for 3D Printing Technology, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200011, ChinaDepartment of Orthopedic Surgery, Renji Hospital, South Campus, Shanghai Jiao Tong University School of Medicine, Shanghai, 201112, China; Co-Corresponding author..Department of Orthopedic Surgery, Shanghai Key Laboratory of Orthopedic Implants, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200011, China; Clinical and Translational Research Center for 3D Printing Technology, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200011, China; Corresponding author. Shanghai Jiao Tong University School of Medicine, Shanghai, 200011, China..Articular cartilage injury is a common disease in the field of orthopedics. Because cartilage has poor self-repairing ability, medical intervention is needed. Using melt electro-writing (MEW) technology, tissue engineering scaffolds with high porosity and high precision can be prepared. However, ordinary materials, especially natural polymer materials, are difficult to print. In this study, gelatin was mixed with poly (lactic-co-glycolic acid) to prepare high-concentration and high-viscosity printer ink, which had good printability and formability. A composite scaffold with full-layer TGF-β1 loading mixed with hydroxyapatite was prepared, and the scaffold was implanted at the cartilage injury site; microfracture surgery was conducted to induce the mesenchyme in the bone marrow. Quality stem cells thereby promoted the repair of damaged cartilage. In summary, this study developed a novel printing method, explored the molding conditions based on MEW printing ink, and constructed a bioactive cartilage repair scaffold. The scaffold can use autologous bone marrow mesenchymal stem cells and induce their differentiation to promote cartilage repair.http://www.sciencedirect.com/science/article/pii/S2452199X20303443Gelatin-based hybrid bilayer scaffoldsMelt electro-writingCartilage injuryBone marrow mesenchymal stem cells |
| spellingShingle | Yu Han Bo Jia Meifei Lian Binbin Sun Qiang Wu Benlin Sun Zhiguang Qiao Kerong Dai High-precision, gelatin-based, hybrid, bilayer scaffolds using melt electro-writing to repair cartilage injury Bioactive Materials Gelatin-based hybrid bilayer scaffolds Melt electro-writing Cartilage injury Bone marrow mesenchymal stem cells |
| title | High-precision, gelatin-based, hybrid, bilayer scaffolds using melt electro-writing to repair cartilage injury |
| title_full | High-precision, gelatin-based, hybrid, bilayer scaffolds using melt electro-writing to repair cartilage injury |
| title_fullStr | High-precision, gelatin-based, hybrid, bilayer scaffolds using melt electro-writing to repair cartilage injury |
| title_full_unstemmed | High-precision, gelatin-based, hybrid, bilayer scaffolds using melt electro-writing to repair cartilage injury |
| title_short | High-precision, gelatin-based, hybrid, bilayer scaffolds using melt electro-writing to repair cartilage injury |
| title_sort | high precision gelatin based hybrid bilayer scaffolds using melt electro writing to repair cartilage injury |
| topic | Gelatin-based hybrid bilayer scaffolds Melt electro-writing Cartilage injury Bone marrow mesenchymal stem cells |
| url | http://www.sciencedirect.com/science/article/pii/S2452199X20303443 |
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