Sequential Analysis of Trace Elements in a Micro Volume Urine Sample Using Inductively Coupled Plasma Mass Spectrometry
In this work, we describe a simple, fast, cheap, accurate and high-throughput method for the determination of Al; V; Cr; Mn; Fe; Co; Cu; Zn; As; Se; Mo; Cd; Sn; Ba; TI; Pb in a micro volume of urine by using inductively coupled mass spectrometry (ICP-MS) equipped with an octupole-based collision cel...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-04-01
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| Series: | Applied Sciences |
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| Online Access: | https://www.mdpi.com/2076-3417/11/9/3740 |
| _version_ | 1797536942264418304 |
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| author | Nico Laur Lars Kaiser Hans-Peter Deigner Ralf Kinscherf |
| author_facet | Nico Laur Lars Kaiser Hans-Peter Deigner Ralf Kinscherf |
| author_sort | Nico Laur |
| collection | DOAJ |
| description | In this work, we describe a simple, fast, cheap, accurate and high-throughput method for the determination of Al; V; Cr; Mn; Fe; Co; Cu; Zn; As; Se; Mo; Cd; Sn; Ba; TI; Pb in a micro volume of urine by using inductively coupled mass spectrometry (ICP-MS) equipped with an octupole-based collision cell. The samples were directly analyzed after a simple acidification with 3% nitric acid. Validation of this method was performed by using certified urine reference material. He and H were used as collision gas for reducing polyatomic interference for most of the measured elements. Finally, we partook in an external quality assurance conducted by ISNTAND e.V. 2. Results show that our high-throughput method is ideal for detecting elements in newborns and infants because of its simplicity, speed, accuracy and low sample volume. |
| first_indexed | 2024-03-10T12:08:00Z |
| format | Article |
| id | doaj.art-d01da531b4044d3cb0a59f997d5191f5 |
| institution | Directory Open Access Journal |
| issn | 2076-3417 |
| language | English |
| last_indexed | 2024-03-10T12:08:00Z |
| publishDate | 2021-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Applied Sciences |
| spelling | doaj.art-d01da531b4044d3cb0a59f997d5191f52023-11-21T16:27:25ZengMDPI AGApplied Sciences2076-34172021-04-01119374010.3390/app11093740Sequential Analysis of Trace Elements in a Micro Volume Urine Sample Using Inductively Coupled Plasma Mass SpectrometryNico Laur0Lars Kaiser1Hans-Peter Deigner2Ralf Kinscherf3Institute of Precision Medicine, Medical and Life Sciences Faculty, Furtwangen University, Jakob-Kienzle-Str. 17, D-78054 VS-Schwenningen, GermanyInstitute of Precision Medicine, Medical and Life Sciences Faculty, Furtwangen University, Jakob-Kienzle-Str. 17, D-78054 VS-Schwenningen, GermanyInstitute of Precision Medicine, Medical and Life Sciences Faculty, Furtwangen University, Jakob-Kienzle-Str. 17, D-78054 VS-Schwenningen, GermanyInstitute of Anatomy and Cell Biology, Department of Medical Cell Biology, University of Marburg, Robert-Koch-Str. 8, D-35037 Marburg, GermanyIn this work, we describe a simple, fast, cheap, accurate and high-throughput method for the determination of Al; V; Cr; Mn; Fe; Co; Cu; Zn; As; Se; Mo; Cd; Sn; Ba; TI; Pb in a micro volume of urine by using inductively coupled mass spectrometry (ICP-MS) equipped with an octupole-based collision cell. The samples were directly analyzed after a simple acidification with 3% nitric acid. Validation of this method was performed by using certified urine reference material. He and H were used as collision gas for reducing polyatomic interference for most of the measured elements. Finally, we partook in an external quality assurance conducted by ISNTAND e.V. 2. Results show that our high-throughput method is ideal for detecting elements in newborns and infants because of its simplicity, speed, accuracy and low sample volume.https://www.mdpi.com/2076-3417/11/9/3740inductively coupled plasma mass spectrometry (ICP-MS)trace elementsmicro volumevalidation |
| spellingShingle | Nico Laur Lars Kaiser Hans-Peter Deigner Ralf Kinscherf Sequential Analysis of Trace Elements in a Micro Volume Urine Sample Using Inductively Coupled Plasma Mass Spectrometry Applied Sciences inductively coupled plasma mass spectrometry (ICP-MS) trace elements micro volume validation |
| title | Sequential Analysis of Trace Elements in a Micro Volume Urine Sample Using Inductively Coupled Plasma Mass Spectrometry |
| title_full | Sequential Analysis of Trace Elements in a Micro Volume Urine Sample Using Inductively Coupled Plasma Mass Spectrometry |
| title_fullStr | Sequential Analysis of Trace Elements in a Micro Volume Urine Sample Using Inductively Coupled Plasma Mass Spectrometry |
| title_full_unstemmed | Sequential Analysis of Trace Elements in a Micro Volume Urine Sample Using Inductively Coupled Plasma Mass Spectrometry |
| title_short | Sequential Analysis of Trace Elements in a Micro Volume Urine Sample Using Inductively Coupled Plasma Mass Spectrometry |
| title_sort | sequential analysis of trace elements in a micro volume urine sample using inductively coupled plasma mass spectrometry |
| topic | inductively coupled plasma mass spectrometry (ICP-MS) trace elements micro volume validation |
| url | https://www.mdpi.com/2076-3417/11/9/3740 |
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