Complex interactions between nicotine and resveratrol in the Drosophila melanogaster wing spot test
Nicotine (NIC) and resveratrol (RES) are chemicals in tobacco and wine, respectively, that are widely consumed concurrently worldwide. NIC is an alkaloid known to be toxic, addictive and to produce oxidative stress, while RES is thought of as an antioxidant with putative health benefits. Oxidative s...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-06-01
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| Series: | Heliyon |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844022010325 |
| _version_ | 1811239175451574272 |
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| author | N.A. Velázquez-Ulloa M.E. Heres-Pulido L.F. Santos-Cruz A. Durán-Díaz L. Castañeda-Partida A. Browning C. Carmona-Alvarado J.C. Estrada-Guzmán G. Ferderer M. Garfias B. Gómez-Loza M.J. Magaña-Acosta H.H. Perry I.E. Dueñas-García |
| author_facet | N.A. Velázquez-Ulloa M.E. Heres-Pulido L.F. Santos-Cruz A. Durán-Díaz L. Castañeda-Partida A. Browning C. Carmona-Alvarado J.C. Estrada-Guzmán G. Ferderer M. Garfias B. Gómez-Loza M.J. Magaña-Acosta H.H. Perry I.E. Dueñas-García |
| author_sort | N.A. Velázquez-Ulloa |
| collection | DOAJ |
| description | Nicotine (NIC) and resveratrol (RES) are chemicals in tobacco and wine, respectively, that are widely consumed concurrently worldwide. NIC is an alkaloid known to be toxic, addictive and to produce oxidative stress, while RES is thought of as an antioxidant with putative health benefits. Oxidative stress can induce genotoxic damage, yet few studies have examined whether NIC is genotoxic in vivo. In vitro studies have shown that RES can ameliorate deleterious effects of NIC. However, RES has been reported to have both antioxidant and pro-oxidant effects, and an in vivo study reported that 0.011 mM RES was genotoxic. We used the Drosophila melanogaster wing spot test to determine whether NIC and RES, first individually and then in combination, were genotoxic and/or altered the cell division. We hypothesized that RES would modulate NIC’s effects. NIC was genotoxic in the standard (ST) cross in a concentration-independent manner, but not genotoxic in the high bioactivation (HB) cross. RES was not genotoxic in either the ST or HB cross at the concentrations tested. We discovered a complex interaction between NIC and RES. Depending on concentration, RES was protective of NIC’s genotoxic damage, RES had no interaction with NIC, or RES had an additive or synergistic effect, increasing NIC’s genotoxic damage. Most NIC, RES, and NIC/RES combinations tested altered the cell division in the ST and HB crosses. Because we used the ST and HB crosses, we demonstrated that genotoxicity and cell division alterations were modulated by the xenobiotic metabolism. These results provide evidence of NIC’s genotoxicity in vivo at specific concentrations. Moreover, NIC’s genotoxicity can be modulated by its interaction with RES in a complex manner, in which their interaction can lead to either increasing NIC’s damage or protecting against it. |
| first_indexed | 2024-04-12T12:55:13Z |
| format | Article |
| id | doaj.art-d022e40a4cb3425481612f07729c92f6 |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-04-12T12:55:13Z |
| publishDate | 2022-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-d022e40a4cb3425481612f07729c92f62022-12-22T03:32:21ZengElsevierHeliyon2405-84402022-06-0186e09744Complex interactions between nicotine and resveratrol in the Drosophila melanogaster wing spot testN.A. Velázquez-Ulloa0M.E. Heres-Pulido1L.F. Santos-Cruz2A. Durán-Díaz3L. Castañeda-Partida4A. Browning5C. Carmona-Alvarado6J.C. Estrada-Guzmán7G. Ferderer8M. Garfias9B. Gómez-Loza10M.J. Magaña-Acosta11H.H. Perry12I.E. Dueñas-García13Biology Department, Lewis & Clark College, Portland, OR, USAGenetic Toxicology Laboratory, Biology, FES Iztacala, Universidad Nacional Autónoma de Mexico (UNAM), Los Barrios N 1, Los Reyes Iztacala, C.P. 54090, Tlalnepantla, Estado de México, MexicoGenetic Toxicology Laboratory, Biology, FES Iztacala, Universidad Nacional Autónoma de Mexico (UNAM), Los Barrios N 1, Los Reyes Iztacala, C.P. 54090, Tlalnepantla, Estado de México, MexicoMathematics, Biology, FES Iztacala, Universidad Nacional Autónoma de Mexico (UNAM), Los Barrios N 1, Los Reyes Iztacala, C.P. 54090, Tlalnepantla, Estado de México, MexicoGenetic Toxicology Laboratory, Biology, FES Iztacala, Universidad Nacional Autónoma de Mexico (UNAM), Los Barrios N 1, Los Reyes Iztacala, C.P. 54090, Tlalnepantla, Estado de México, MexicoBiology Department, Lewis & Clark College, Portland, OR, USAGenetic Toxicology Laboratory, Biology, FES Iztacala, Universidad Nacional Autónoma de Mexico (UNAM), Los Barrios N 1, Los Reyes Iztacala, C.P. 54090, Tlalnepantla, Estado de México, MexicoGenetic Toxicology Laboratory, Biology, FES Iztacala, Universidad Nacional Autónoma de Mexico (UNAM), Los Barrios N 1, Los Reyes Iztacala, C.P. 54090, Tlalnepantla, Estado de México, MexicoBiology Department, Lewis & Clark College, Portland, OR, USABiology Department, Lewis & Clark College, Portland, OR, USA; Animal and Comparative Biomedical Sciences, University of Arizona, Tucson, AZ, USAGenetic Toxicology Laboratory, Biology, FES Iztacala, Universidad Nacional Autónoma de Mexico (UNAM), Los Barrios N 1, Los Reyes Iztacala, C.P. 54090, Tlalnepantla, Estado de México, MexicoGenetic Toxicology Laboratory, Biology, FES Iztacala, Universidad Nacional Autónoma de Mexico (UNAM), Los Barrios N 1, Los Reyes Iztacala, C.P. 54090, Tlalnepantla, Estado de México, Mexico; Department of Developmental Genetics & Molecular Physiology, Universidad Nacional Autónoma de México. Av Universidad, 2001, Col Chamilpa, Cuernavaca, MexicoBiology Department, Lewis & Clark College, Portland, OR, USAGenetic Toxicology Laboratory, Biology, FES Iztacala, Universidad Nacional Autónoma de Mexico (UNAM), Los Barrios N 1, Los Reyes Iztacala, C.P. 54090, Tlalnepantla, Estado de México, Mexico; Corresponding author.Nicotine (NIC) and resveratrol (RES) are chemicals in tobacco and wine, respectively, that are widely consumed concurrently worldwide. NIC is an alkaloid known to be toxic, addictive and to produce oxidative stress, while RES is thought of as an antioxidant with putative health benefits. Oxidative stress can induce genotoxic damage, yet few studies have examined whether NIC is genotoxic in vivo. In vitro studies have shown that RES can ameliorate deleterious effects of NIC. However, RES has been reported to have both antioxidant and pro-oxidant effects, and an in vivo study reported that 0.011 mM RES was genotoxic. We used the Drosophila melanogaster wing spot test to determine whether NIC and RES, first individually and then in combination, were genotoxic and/or altered the cell division. We hypothesized that RES would modulate NIC’s effects. NIC was genotoxic in the standard (ST) cross in a concentration-independent manner, but not genotoxic in the high bioactivation (HB) cross. RES was not genotoxic in either the ST or HB cross at the concentrations tested. We discovered a complex interaction between NIC and RES. Depending on concentration, RES was protective of NIC’s genotoxic damage, RES had no interaction with NIC, or RES had an additive or synergistic effect, increasing NIC’s genotoxic damage. Most NIC, RES, and NIC/RES combinations tested altered the cell division in the ST and HB crosses. Because we used the ST and HB crosses, we demonstrated that genotoxicity and cell division alterations were modulated by the xenobiotic metabolism. These results provide evidence of NIC’s genotoxicity in vivo at specific concentrations. Moreover, NIC’s genotoxicity can be modulated by its interaction with RES in a complex manner, in which their interaction can lead to either increasing NIC’s damage or protecting against it.http://www.sciencedirect.com/science/article/pii/S2405844022010325NicotineResveratrolGenotoxic damageDrosophila wing spot testXenobiotic metabolism |
| spellingShingle | N.A. Velázquez-Ulloa M.E. Heres-Pulido L.F. Santos-Cruz A. Durán-Díaz L. Castañeda-Partida A. Browning C. Carmona-Alvarado J.C. Estrada-Guzmán G. Ferderer M. Garfias B. Gómez-Loza M.J. Magaña-Acosta H.H. Perry I.E. Dueñas-García Complex interactions between nicotine and resveratrol in the Drosophila melanogaster wing spot test Heliyon Nicotine Resveratrol Genotoxic damage Drosophila wing spot test Xenobiotic metabolism |
| title | Complex interactions between nicotine and resveratrol in the Drosophila melanogaster wing spot test |
| title_full | Complex interactions between nicotine and resveratrol in the Drosophila melanogaster wing spot test |
| title_fullStr | Complex interactions between nicotine and resveratrol in the Drosophila melanogaster wing spot test |
| title_full_unstemmed | Complex interactions between nicotine and resveratrol in the Drosophila melanogaster wing spot test |
| title_short | Complex interactions between nicotine and resveratrol in the Drosophila melanogaster wing spot test |
| title_sort | complex interactions between nicotine and resveratrol in the drosophila melanogaster wing spot test |
| topic | Nicotine Resveratrol Genotoxic damage Drosophila wing spot test Xenobiotic metabolism |
| url | http://www.sciencedirect.com/science/article/pii/S2405844022010325 |
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