Development of a MEL Cell-Derived Allograft Mouse Model for Cancer Research
Murine erythroleukemia (MEL) cells are often employed as a model to dissect mechanisms of erythropoiesis and erythroleukemia in vitro. Here, an allograft model using MEL cells resulting in splenomegaly was established to develop a diagnostic model for isolation/quantification of metastatic cells, an...
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| Format: | Article |
| Language: | English |
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MDPI AG
2019-11-01
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| Series: | Cancers |
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| Online Access: | https://www.mdpi.com/2072-6694/11/11/1707 |
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| author | Min Young Kim Sungwoo Choi Seol Eui Lee Ji Sook Kim Seung Han Son Young Soo Lim Bang-Jin Kim Buom-Yong Ryu Vladimir N. Uversky Young Jin Lee Chul Geun Kim |
| author_facet | Min Young Kim Sungwoo Choi Seol Eui Lee Ji Sook Kim Seung Han Son Young Soo Lim Bang-Jin Kim Buom-Yong Ryu Vladimir N. Uversky Young Jin Lee Chul Geun Kim |
| author_sort | Min Young Kim |
| collection | DOAJ |
| description | Murine erythroleukemia (MEL) cells are often employed as a model to dissect mechanisms of erythropoiesis and erythroleukemia in vitro. Here, an allograft model using MEL cells resulting in splenomegaly was established to develop a diagnostic model for isolation/quantification of metastatic cells, anti-cancer drug screening, and evaluation of the tumorigenic or metastatic potentials of molecules in vivo. In this animal model, circulating MEL cells from the blood stream were successfully isolated and quantified with an additional in vitro cultivation step. In terms of the molecular-pathological analysis, we were able to successfully evaluate the functional discrimination between methyl-CpG-binding domain 2 (Mbd2) and p66α in erythroid differentiation, and tumorigenic potential in spleen and blood stream of allograft model mice. In addition, we found that the number of circulating MEL cells in anti-cancer drug-treated mice was dose-dependently decreased. Our data demonstrate that the newly established allograft model is useful to dissect erythroleukemia pathologies and non-invasively provides valuable means for isolation of metastatic cells, screening of anti-cancer drugs, and evaluation of the tumorigenic potentials. |
| first_indexed | 2024-03-12T06:07:06Z |
| format | Article |
| id | doaj.art-d02524d566254c02b531d233aca90799 |
| institution | Directory Open Access Journal |
| issn | 2072-6694 |
| language | English |
| last_indexed | 2024-03-12T06:07:06Z |
| publishDate | 2019-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cancers |
| spelling | doaj.art-d02524d566254c02b531d233aca907992023-09-03T03:35:35ZengMDPI AGCancers2072-66942019-11-011111170710.3390/cancers11111707cancers11111707Development of a MEL Cell-Derived Allograft Mouse Model for Cancer ResearchMin Young Kim0Sungwoo Choi1Seol Eui Lee2Ji Sook Kim3Seung Han Son4Young Soo Lim5Bang-Jin Kim6Buom-Yong Ryu7Vladimir N. Uversky8Young Jin Lee9Chul Geun Kim10Department of Life Science, College of Natural Sciences, Hanyang University, Seoul 04763, KoreaDepartment of Life Science, College of Natural Sciences, Hanyang University, Seoul 04763, KoreaDepartment of Life Science, College of Natural Sciences, Hanyang University, Seoul 04763, KoreaDepartment of Life Science, College of Natural Sciences, Hanyang University, Seoul 04763, KoreaDepartment of Life Science, College of Natural Sciences, Hanyang University, Seoul 04763, KoreaDepartment of Life Science, College of Natural Sciences, Hanyang University, Seoul 04763, KoreaDepartment of Animal Science & Technology, Chung-Ang University, Ansung, Gyeonggi-do 17546, KoreaDepartment of Animal Science & Technology, Chung-Ang University, Ansung, Gyeonggi-do 17546, KoreaDepartment of Molecular Medicine, USF Health Byrd Alzheimer’s Research Institute, Morsani College of Medicine, University of South Florida, Tampa, FL 33612, USAInstitute of Pharmaceutical Science and Technology, Department of Pharmacy, Hanyang University, Ansan, Gyeonggi-do 15588, KoreaDepartment of Life Science, College of Natural Sciences, Hanyang University, Seoul 04763, KoreaMurine erythroleukemia (MEL) cells are often employed as a model to dissect mechanisms of erythropoiesis and erythroleukemia in vitro. Here, an allograft model using MEL cells resulting in splenomegaly was established to develop a diagnostic model for isolation/quantification of metastatic cells, anti-cancer drug screening, and evaluation of the tumorigenic or metastatic potentials of molecules in vivo. In this animal model, circulating MEL cells from the blood stream were successfully isolated and quantified with an additional in vitro cultivation step. In terms of the molecular-pathological analysis, we were able to successfully evaluate the functional discrimination between methyl-CpG-binding domain 2 (Mbd2) and p66α in erythroid differentiation, and tumorigenic potential in spleen and blood stream of allograft model mice. In addition, we found that the number of circulating MEL cells in anti-cancer drug-treated mice was dose-dependently decreased. Our data demonstrate that the newly established allograft model is useful to dissect erythroleukemia pathologies and non-invasively provides valuable means for isolation of metastatic cells, screening of anti-cancer drugs, and evaluation of the tumorigenic potentials.https://www.mdpi.com/2072-6694/11/11/1707circulating tumor cellserythroleukemiaallograftliquid biopsycancer treatment |
| spellingShingle | Min Young Kim Sungwoo Choi Seol Eui Lee Ji Sook Kim Seung Han Son Young Soo Lim Bang-Jin Kim Buom-Yong Ryu Vladimir N. Uversky Young Jin Lee Chul Geun Kim Development of a MEL Cell-Derived Allograft Mouse Model for Cancer Research Cancers circulating tumor cells erythroleukemia allograft liquid biopsy cancer treatment |
| title | Development of a MEL Cell-Derived Allograft Mouse Model for Cancer Research |
| title_full | Development of a MEL Cell-Derived Allograft Mouse Model for Cancer Research |
| title_fullStr | Development of a MEL Cell-Derived Allograft Mouse Model for Cancer Research |
| title_full_unstemmed | Development of a MEL Cell-Derived Allograft Mouse Model for Cancer Research |
| title_short | Development of a MEL Cell-Derived Allograft Mouse Model for Cancer Research |
| title_sort | development of a mel cell derived allograft mouse model for cancer research |
| topic | circulating tumor cells erythroleukemia allograft liquid biopsy cancer treatment |
| url | https://www.mdpi.com/2072-6694/11/11/1707 |
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