Expression of several deubiquitinase and tyrosine phosphatase genes involved in inflammatory response in lymphoma
Lymphoma represents a heterogeneous group of cancers affecting the lymphatic system, encompassing nonHodgkin lymphoma (NHL) and Hodgkin lymphoma (HL). The majority of NHL cases originate from B cells. HL is characterized by the presence of rare Hodgkin and Reed-Sternberg cells. Within the context o...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Vietnam Ministry of Science and Technology
2024-03-01
|
| Series: | Vietnam Journal of Science, Technology and Engineering |
| Subjects: | |
| Online Access: | https://vietnamscience.vjst.vn/index.php/vjste/article/view/1189 |
| _version_ | 1827316707288416256 |
|---|---|
| author | Nguyen Hoang Giang Nguyen Trong Ha Nguyen Thi Xuan |
| author_facet | Nguyen Hoang Giang Nguyen Trong Ha Nguyen Thi Xuan |
| author_sort | Nguyen Hoang Giang |
| collection | DOAJ |
| description |
Lymphoma represents a heterogeneous group of cancers affecting the lymphatic system, encompassing nonHodgkin lymphoma (NHL) and Hodgkin lymphoma (HL). The majority of NHL cases originate from B cells. HL is characterized by the presence of rare Hodgkin and Reed-Sternberg cells. Within the context of the inflammatory response, two SH2 domain-containing protein tyrosine phosphatases (SHPs), namely SHP-1 and SHP-2, serve as negative regulators. Deubiquitinating enzymes (DUBs) play a crucial role in inhibiting NF- κB activation in response to various stimuli. This study focuses on the DUBs OTUB1, OTUB2, and Cezanne. Blood samples were collected from 50 NHL patients, 26 HL patients, and 50 healthy individuals. Quantitative RT-PCR was employed to assess the mRNA expression of OTUB1, OTUB2, Cezanne, SHP-1, and SHP-2, while ELISA was used to determine IL-6 and CA125 concentrations. The results revealed that the mRNA level of OTUB1 was significantly downregulated in NHL patients but not in HL patients. Notably, Cezanne expression was downregulated in lymphoma patients, with significantly lower levels observed in HL compared to NHL patients. Furthermore, SHP-1 mRNA expression was significantly lower in the NHL group compared to the HL group or healthy individuals. Conversely, SHP-2 gene expression was upregulated in NHL patients but remained unchanged in HL patients. In conclusion, these findings highlight significant differences in the expressions of DUB and SHP genes and the inflammatory response in lymphoma patients. This study provides a foundation for further investigation into the roles of DUBs and tyrosine phosphatases in regulating the functional activation of lymphoma cells.
|
| first_indexed | 2024-04-24T23:19:57Z |
| format | Article |
| id | doaj.art-d0264f89a6ba4e9caab939ce178704a8 |
| institution | Directory Open Access Journal |
| issn | 2525-2461 2615-9937 |
| language | English |
| last_indexed | 2024-04-24T23:19:57Z |
| publishDate | 2024-03-01 |
| publisher | Vietnam Ministry of Science and Technology |
| record_format | Article |
| series | Vietnam Journal of Science, Technology and Engineering |
| spelling | doaj.art-d0264f89a6ba4e9caab939ce178704a82024-03-16T23:50:08ZengVietnam Ministry of Science and TechnologyVietnam Journal of Science, Technology and Engineering2525-24612615-99372024-03-0166110.31276/VJSTE.66(1).90-95Expression of several deubiquitinase and tyrosine phosphatase genes involved in inflammatory response in lymphomaNguyen Hoang GiangNguyen Trong HaNguyen Thi Xuan Lymphoma represents a heterogeneous group of cancers affecting the lymphatic system, encompassing nonHodgkin lymphoma (NHL) and Hodgkin lymphoma (HL). The majority of NHL cases originate from B cells. HL is characterized by the presence of rare Hodgkin and Reed-Sternberg cells. Within the context of the inflammatory response, two SH2 domain-containing protein tyrosine phosphatases (SHPs), namely SHP-1 and SHP-2, serve as negative regulators. Deubiquitinating enzymes (DUBs) play a crucial role in inhibiting NF- κB activation in response to various stimuli. This study focuses on the DUBs OTUB1, OTUB2, and Cezanne. Blood samples were collected from 50 NHL patients, 26 HL patients, and 50 healthy individuals. Quantitative RT-PCR was employed to assess the mRNA expression of OTUB1, OTUB2, Cezanne, SHP-1, and SHP-2, while ELISA was used to determine IL-6 and CA125 concentrations. The results revealed that the mRNA level of OTUB1 was significantly downregulated in NHL patients but not in HL patients. Notably, Cezanne expression was downregulated in lymphoma patients, with significantly lower levels observed in HL compared to NHL patients. Furthermore, SHP-1 mRNA expression was significantly lower in the NHL group compared to the HL group or healthy individuals. Conversely, SHP-2 gene expression was upregulated in NHL patients but remained unchanged in HL patients. In conclusion, these findings highlight significant differences in the expressions of DUB and SHP genes and the inflammatory response in lymphoma patients. This study provides a foundation for further investigation into the roles of DUBs and tyrosine phosphatases in regulating the functional activation of lymphoma cells. https://vietnamscience.vjst.vn/index.php/vjste/article/view/1189CA125deubiquitinating enzymesIL-6lymphomatyrosine phosphatases |
| spellingShingle | Nguyen Hoang Giang Nguyen Trong Ha Nguyen Thi Xuan Expression of several deubiquitinase and tyrosine phosphatase genes involved in inflammatory response in lymphoma Vietnam Journal of Science, Technology and Engineering CA125 deubiquitinating enzymes IL-6 lymphoma tyrosine phosphatases |
| title | Expression of several deubiquitinase and tyrosine phosphatase genes involved in inflammatory response in lymphoma |
| title_full | Expression of several deubiquitinase and tyrosine phosphatase genes involved in inflammatory response in lymphoma |
| title_fullStr | Expression of several deubiquitinase and tyrosine phosphatase genes involved in inflammatory response in lymphoma |
| title_full_unstemmed | Expression of several deubiquitinase and tyrosine phosphatase genes involved in inflammatory response in lymphoma |
| title_short | Expression of several deubiquitinase and tyrosine phosphatase genes involved in inflammatory response in lymphoma |
| title_sort | expression of several deubiquitinase and tyrosine phosphatase genes involved in inflammatory response in lymphoma |
| topic | CA125 deubiquitinating enzymes IL-6 lymphoma tyrosine phosphatases |
| url | https://vietnamscience.vjst.vn/index.php/vjste/article/view/1189 |
| work_keys_str_mv | AT nguyenhoanggiang expressionofseveraldeubiquitinaseandtyrosinephosphatasegenesinvolvedininflammatoryresponseinlymphoma AT nguyentrongha expressionofseveraldeubiquitinaseandtyrosinephosphatasegenesinvolvedininflammatoryresponseinlymphoma AT nguyenthixuan expressionofseveraldeubiquitinaseandtyrosinephosphatasegenesinvolvedininflammatoryresponseinlymphoma |