Identification, cloning and environmental factors modulation of a αβ defensin from the Lessepsian invasive mussel Brachidontes pharaonis (Bivalvia: Mytilidae)
Immunological effectors of invasive species playing a role in addressing new colonization are still poorly studied. In the present study the cDNA sequence of the defensin from a Lessepsian invasive species, the Red Sea mussel Brachidontes pharaonis, was cloned using RACE method. Defensins are a c...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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University of Modena and Reggio Emilia
2015-10-01
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| Series: | Invertebrate Survival Journal |
| Subjects: | |
| Online Access: | http://www.isj.unimo.it/articoli/ISJ393.pdf |
| _version_ | 1811259809476902912 |
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| author | MG Parisi A Vizzini M Toubiana G Sarà M Cammarata |
| author_facet | MG Parisi A Vizzini M Toubiana G Sarà M Cammarata |
| author_sort | MG Parisi |
| collection | DOAJ |
| description | Immunological effectors of invasive species playing a role in addressing new colonization are still
poorly studied. In the present study the cDNA sequence of the defensin from a Lessepsian invasive
species, the Red Sea mussel Brachidontes pharaonis, was cloned using RACE method. Defensins
are a class of widely known antimicrobial peptides (AMPs), oligopeptides with a broad spectrum of
targeted organisms ranging from viruses to parasites. Analysis of BpDef sequence (262 bp) revealed
the presence of an ORF coding for 81 amino acids. The full-length amino acid sequence showed the
highest similarity to antimicrobial peptides MGD1 and MGD2 sequence from Mytilus galloprovincialis.
Phylogenetic analysis suggested that BpDef belongs to the αβ defensin AMPs with a typical domain
structurally characterized by a α helix and two β sheets. BpDef mRNA is located in circulating
hemocytes with small intra-cytoplasmic granules and with large granules. The transcription of defensin
gene was modulated by the stress from temperatures and oxygenation condition. Temperatures of 20
°C did not stimulate a BpDef transcription over a short time. At 30 °C the kinetics of BpDef gene
transcription showed up regulation after one day, while it was down regulated after six days, both
under normoxia and hypoxia conditions. |
| first_indexed | 2024-04-12T18:37:10Z |
| format | Article |
| id | doaj.art-d0280e854b944599bbfb1244e511d786 |
| institution | Directory Open Access Journal |
| issn | 1824-307X |
| language | English |
| last_indexed | 2024-04-12T18:37:10Z |
| publishDate | 2015-10-01 |
| publisher | University of Modena and Reggio Emilia |
| record_format | Article |
| series | Invertebrate Survival Journal |
| spelling | doaj.art-d0280e854b944599bbfb1244e511d7862022-12-22T03:20:54ZengUniversity of Modena and Reggio EmiliaInvertebrate Survival Journal1824-307X2015-10-0112264273Identification, cloning and environmental factors modulation of a αβ defensin from the Lessepsian invasive mussel Brachidontes pharaonis (Bivalvia: Mytilidae)MG Parisi0A Vizzini1M Toubiana2G Sarà3M Cammarata4Laboratory of Marine Immunobiology Department of Biological, Chemical and Pharmaceutical Sciences and Technologies, University of Palermo, ItalyLaboratory of Marine Immunobiology Department of Biological, Chemical and Pharmaceutical Sciences and Technologies, University of Palermo, ItalyEcologie des Systèmes Marins et Côtiers (EcoSym), Université Montpellier 2-CNRS, cc 093, place E. Bataillon, F-34095 Montpellier cedex 05, FranceLaboratory of Experimental Ecology and Behaviour Department of Earth and Marine Sciences, University of Palermo, ItalyLaboratory of Marine Immunobiology Department of Biological, Chemical and Pharmaceutical Sciences and Technologies, University of Palermo, ItalyImmunological effectors of invasive species playing a role in addressing new colonization are still poorly studied. In the present study the cDNA sequence of the defensin from a Lessepsian invasive species, the Red Sea mussel Brachidontes pharaonis, was cloned using RACE method. Defensins are a class of widely known antimicrobial peptides (AMPs), oligopeptides with a broad spectrum of targeted organisms ranging from viruses to parasites. Analysis of BpDef sequence (262 bp) revealed the presence of an ORF coding for 81 amino acids. The full-length amino acid sequence showed the highest similarity to antimicrobial peptides MGD1 and MGD2 sequence from Mytilus galloprovincialis. Phylogenetic analysis suggested that BpDef belongs to the αβ defensin AMPs with a typical domain structurally characterized by a α helix and two β sheets. BpDef mRNA is located in circulating hemocytes with small intra-cytoplasmic granules and with large granules. The transcription of defensin gene was modulated by the stress from temperatures and oxygenation condition. Temperatures of 20 °C did not stimulate a BpDef transcription over a short time. At 30 °C the kinetics of BpDef gene transcription showed up regulation after one day, while it was down regulated after six days, both under normoxia and hypoxia conditions.http://www.isj.unimo.it/articoli/ISJ393.pdfBrachidontes pharaonis; Antimicrobial peptides (AMPs); defensin; Lessepsian mussel; environmental stress effect |
| spellingShingle | MG Parisi A Vizzini M Toubiana G Sarà M Cammarata Identification, cloning and environmental factors modulation of a αβ defensin from the Lessepsian invasive mussel Brachidontes pharaonis (Bivalvia: Mytilidae) Invertebrate Survival Journal Brachidontes pharaonis; Antimicrobial peptides (AMPs); defensin; Lessepsian mussel; environmental stress effect |
| title | Identification, cloning and environmental factors modulation of a αβ defensin from the Lessepsian invasive mussel Brachidontes pharaonis (Bivalvia: Mytilidae) |
| title_full | Identification, cloning and environmental factors modulation of a αβ defensin from the Lessepsian invasive mussel Brachidontes pharaonis (Bivalvia: Mytilidae) |
| title_fullStr | Identification, cloning and environmental factors modulation of a αβ defensin from the Lessepsian invasive mussel Brachidontes pharaonis (Bivalvia: Mytilidae) |
| title_full_unstemmed | Identification, cloning and environmental factors modulation of a αβ defensin from the Lessepsian invasive mussel Brachidontes pharaonis (Bivalvia: Mytilidae) |
| title_short | Identification, cloning and environmental factors modulation of a αβ defensin from the Lessepsian invasive mussel Brachidontes pharaonis (Bivalvia: Mytilidae) |
| title_sort | identification cloning and environmental factors modulation of a αβ defensin from the lessepsian invasive mussel brachidontes pharaonis bivalvia mytilidae |
| topic | Brachidontes pharaonis; Antimicrobial peptides (AMPs); defensin; Lessepsian mussel; environmental stress effect |
| url | http://www.isj.unimo.it/articoli/ISJ393.pdf |
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