A Comprehensive Phytochemical Analysis of <i>Sideritis scardica</i> Infusion Using Orbitrap UHPLC-HRMS
<i>Sideritis scardica</i> Griseb, also known as “mountain tea” and “Olympus tea” (Lamiaceae family) is an endemic plant from the mountainous regions of the Balkan Peninsula. In this study, we focused on an in-depth phytochemical analysis of <i>S. scardica</i> infusion using u...
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| Format: | Article |
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MDPI AG
2023-12-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/29/1/204 |
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| author | Dimitrina Zheleva-Dimitrova Yulian Voynikov Reneta Gevrenova Vessela Balabanova |
| author_facet | Dimitrina Zheleva-Dimitrova Yulian Voynikov Reneta Gevrenova Vessela Balabanova |
| author_sort | Dimitrina Zheleva-Dimitrova |
| collection | DOAJ |
| description | <i>Sideritis scardica</i> Griseb, also known as “mountain tea” and “Olympus tea” (Lamiaceae family) is an endemic plant from the mountainous regions of the Balkan Peninsula. In this study, we focused on an in-depth phytochemical analysis of <i>S. scardica</i> infusion using ultra-high-performance liquid chromatography hyphenated with high-resolution mass spectrometry (UHPLC–HRMS). Quantitative determination of the main secondary metabolites was carried out by UHPLC–HRMS analyses using the external standard method. The results revealed more than 100 metabolites, including five sugar acids and saccharides, 21 carboxylic, hydroxybenzoic, hydroxycinnamic acids, and derivatives, 15 acylquinic acids, 10 phenylpropanoid glycosides, four iridoid glycosides, 28 flavonoids, seven fatty acids, and four organosulfur compounds. Furthermore, a dereplication and fragmentation patterns of five caffeic acids oligomers and four acylhexaric acids was performed for the first time in <i>S. scardica</i>. Regarding the quantitative analysis, the phenylethanoid verbascoside (<b>53</b>) (151.54 ± 10.86 mg/g lyophilized infusion, li), the glycosides of isoscutellarein (<b>78</b>) (151.70 ± 14.78 mg/g li), methylisoscutelarein (<b>82</b>) (107.4 ± 9.07 mg/g li), and hypolaetin (<b>79</b>) (78.33 ± 3.29 mg/g li), as well as caffeic acid (<b>20</b>) (87.25 ± 6.54 mg/g li), were found to be the major compounds in <i>S. scardica</i> infusion. The performed state-of-the-art phytochemical analysis of <i>S. scardica</i> provides additional knowledge for the chemical constituents and usage of this valuable medicinal plant. |
| first_indexed | 2024-03-08T15:01:47Z |
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| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-08T15:01:47Z |
| publishDate | 2023-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-d02883b51e204e489475131ea1d88ee22024-01-10T15:04:29ZengMDPI AGMolecules1420-30492023-12-0129120410.3390/molecules29010204A Comprehensive Phytochemical Analysis of <i>Sideritis scardica</i> Infusion Using Orbitrap UHPLC-HRMSDimitrina Zheleva-Dimitrova0Yulian Voynikov1Reneta Gevrenova2Vessela Balabanova3Department of Pharmacognosy, Faculty of Pharmacy, Medical University of Sofia, 1000 Sofia, BulgariaDepartment of Chemistry, Faculty of Pharmacy, Medical University of Sofia, 1000 Sofia, BulgariaDepartment of Pharmacognosy, Faculty of Pharmacy, Medical University of Sofia, 1000 Sofia, BulgariaDepartment of Pharmacognosy, Faculty of Pharmacy, Medical University of Sofia, 1000 Sofia, Bulgaria<i>Sideritis scardica</i> Griseb, also known as “mountain tea” and “Olympus tea” (Lamiaceae family) is an endemic plant from the mountainous regions of the Balkan Peninsula. In this study, we focused on an in-depth phytochemical analysis of <i>S. scardica</i> infusion using ultra-high-performance liquid chromatography hyphenated with high-resolution mass spectrometry (UHPLC–HRMS). Quantitative determination of the main secondary metabolites was carried out by UHPLC–HRMS analyses using the external standard method. The results revealed more than 100 metabolites, including five sugar acids and saccharides, 21 carboxylic, hydroxybenzoic, hydroxycinnamic acids, and derivatives, 15 acylquinic acids, 10 phenylpropanoid glycosides, four iridoid glycosides, 28 flavonoids, seven fatty acids, and four organosulfur compounds. Furthermore, a dereplication and fragmentation patterns of five caffeic acids oligomers and four acylhexaric acids was performed for the first time in <i>S. scardica</i>. Regarding the quantitative analysis, the phenylethanoid verbascoside (<b>53</b>) (151.54 ± 10.86 mg/g lyophilized infusion, li), the glycosides of isoscutellarein (<b>78</b>) (151.70 ± 14.78 mg/g li), methylisoscutelarein (<b>82</b>) (107.4 ± 9.07 mg/g li), and hypolaetin (<b>79</b>) (78.33 ± 3.29 mg/g li), as well as caffeic acid (<b>20</b>) (87.25 ± 6.54 mg/g li), were found to be the major compounds in <i>S. scardica</i> infusion. The performed state-of-the-art phytochemical analysis of <i>S. scardica</i> provides additional knowledge for the chemical constituents and usage of this valuable medicinal plant.https://www.mdpi.com/1420-3049/29/1/204<i>Sideritis scardica</i> GrisebUHPLC–HRMSphytochemical analysis |
| spellingShingle | Dimitrina Zheleva-Dimitrova Yulian Voynikov Reneta Gevrenova Vessela Balabanova A Comprehensive Phytochemical Analysis of <i>Sideritis scardica</i> Infusion Using Orbitrap UHPLC-HRMS Molecules <i>Sideritis scardica</i> Griseb UHPLC–HRMS phytochemical analysis |
| title | A Comprehensive Phytochemical Analysis of <i>Sideritis scardica</i> Infusion Using Orbitrap UHPLC-HRMS |
| title_full | A Comprehensive Phytochemical Analysis of <i>Sideritis scardica</i> Infusion Using Orbitrap UHPLC-HRMS |
| title_fullStr | A Comprehensive Phytochemical Analysis of <i>Sideritis scardica</i> Infusion Using Orbitrap UHPLC-HRMS |
| title_full_unstemmed | A Comprehensive Phytochemical Analysis of <i>Sideritis scardica</i> Infusion Using Orbitrap UHPLC-HRMS |
| title_short | A Comprehensive Phytochemical Analysis of <i>Sideritis scardica</i> Infusion Using Orbitrap UHPLC-HRMS |
| title_sort | comprehensive phytochemical analysis of i sideritis scardica i infusion using orbitrap uhplc hrms |
| topic | <i>Sideritis scardica</i> Griseb UHPLC–HRMS phytochemical analysis |
| url | https://www.mdpi.com/1420-3049/29/1/204 |
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