Detection of Mycoplasma bovis in bulk tank milk samples by nested PCR in Mashhad, Iran
Mycoplasma bovis is a highly contagious major mastitis pathogen with multiple clinical presentations in dairy cows. This kind of mastitis does not respond to available antibiotics and actually there is no effective therapy for this infection, thus the best way of prevention and control is to diagnos...
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| Format: | Article |
| Language: | English |
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Ferdowsi University of Mashhad
2017-01-01
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| Series: | The Iranian Journal of Veterinary Science and Technology |
| Subjects: | |
| Online Access: | https://ijvst.um.ac.ir/article_29463_64da64165bfd0ee0f165766c8eb5fffb.pdf |
| _version_ | 1818272546202583040 |
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| author | Mehran Dabiri Pezhman Mirshokraei Mehrnaz Rad babak Khoramian |
| author_facet | Mehran Dabiri Pezhman Mirshokraei Mehrnaz Rad babak Khoramian |
| author_sort | Mehran Dabiri |
| collection | DOAJ |
| description | Mycoplasma bovis is a highly contagious major mastitis pathogen with multiple clinical presentations in dairy cows. This kind of mastitis does not respond to available antibiotics and actually there is no effective therapy for this infection, thus the best way of prevention and control is to diagnose and cull the affected cows in the herd. The objective of this study was to detect Mycoplasma bovis in bulk tank milk samples by nested PCR in Mashhad, Iran. One hundred and four fresh bulk tank milk samples from 52 dairy herds were collected four weeks apart. Mycoplasma bovis was not detected from any of them by either direct PCR on milk or after enrichment in modified Hayflick’s broth. Two other mycoplasma species were detected after enrichment and one other mycoplasma
species without enrichment by mycoplasma spp. primer. Sequencing of the PCR products from two positive samples confirmed the presence of mycoplasma that were Mycoplasma canadense and Mycoplasma yeatsii. |
| first_indexed | 2024-12-12T21:43:47Z |
| format | Article |
| id | doaj.art-d02ccc1c6cf44377a5eda688d18cafbc |
| institution | Directory Open Access Journal |
| issn | 2008-465X 2423-6306 |
| language | English |
| last_indexed | 2024-12-12T21:43:47Z |
| publishDate | 2017-01-01 |
| publisher | Ferdowsi University of Mashhad |
| record_format | Article |
| series | The Iranian Journal of Veterinary Science and Technology |
| spelling | doaj.art-d02ccc1c6cf44377a5eda688d18cafbc2022-12-22T00:10:59ZengFerdowsi University of MashhadThe Iranian Journal of Veterinary Science and Technology2008-465X2423-63062017-01-0192283210.22067/veterinary.v9i2.5767829463Detection of Mycoplasma bovis in bulk tank milk samples by nested PCR in Mashhad, IranMehran Dabiri0Pezhman Mirshokraei1Mehrnaz Rad2babak Khoramian3Ferdowsi University of MashhadFerdowsi University of MashhadFerdowsi University of MashhadFerdowsi University of MashhadMycoplasma bovis is a highly contagious major mastitis pathogen with multiple clinical presentations in dairy cows. This kind of mastitis does not respond to available antibiotics and actually there is no effective therapy for this infection, thus the best way of prevention and control is to diagnose and cull the affected cows in the herd. The objective of this study was to detect Mycoplasma bovis in bulk tank milk samples by nested PCR in Mashhad, Iran. One hundred and four fresh bulk tank milk samples from 52 dairy herds were collected four weeks apart. Mycoplasma bovis was not detected from any of them by either direct PCR on milk or after enrichment in modified Hayflick’s broth. Two other mycoplasma species were detected after enrichment and one other mycoplasma species without enrichment by mycoplasma spp. primer. Sequencing of the PCR products from two positive samples confirmed the presence of mycoplasma that were Mycoplasma canadense and Mycoplasma yeatsii.https://ijvst.um.ac.ir/article_29463_64da64165bfd0ee0f165766c8eb5fffb.pdfmycoplasma bovismilk tankmashhadnested pcr |
| spellingShingle | Mehran Dabiri Pezhman Mirshokraei Mehrnaz Rad babak Khoramian Detection of Mycoplasma bovis in bulk tank milk samples by nested PCR in Mashhad, Iran The Iranian Journal of Veterinary Science and Technology mycoplasma bovis milk tank mashhad nested pcr |
| title | Detection of Mycoplasma bovis in bulk tank milk samples by nested PCR in Mashhad, Iran |
| title_full | Detection of Mycoplasma bovis in bulk tank milk samples by nested PCR in Mashhad, Iran |
| title_fullStr | Detection of Mycoplasma bovis in bulk tank milk samples by nested PCR in Mashhad, Iran |
| title_full_unstemmed | Detection of Mycoplasma bovis in bulk tank milk samples by nested PCR in Mashhad, Iran |
| title_short | Detection of Mycoplasma bovis in bulk tank milk samples by nested PCR in Mashhad, Iran |
| title_sort | detection of mycoplasma bovis in bulk tank milk samples by nested pcr in mashhad iran |
| topic | mycoplasma bovis milk tank mashhad nested pcr |
| url | https://ijvst.um.ac.ir/article_29463_64da64165bfd0ee0f165766c8eb5fffb.pdf |
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