β‐defensin‐2 in breast milk displays a broad antimicrobial activity against pathogenic bacteria
Objective: To describe the antimicrobial activity of β‐defensin‐2 produced in the mammary gland and secreted in human breast milk. Methods: The peptide production was performed by DNA cloning. β‐defensin‐2 levels were quantified in 61 colostrum samples and 39 mature milk samples from healthy donors,...
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| Format: | Article |
| Language: | Portuguese |
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Brazilian Society of Pediatrics
2015-01-01
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| Series: | Jornal de Pediatria (Versão em Português) |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2255553614001554 |
| _version_ | 1828159920853221376 |
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| author | Joanna Baricelli Miguel A. Rocafull Desiree Vázquez Betsi Bastidas Estalina Báez‐Ramirez Luz E. Thomas |
| author_facet | Joanna Baricelli Miguel A. Rocafull Desiree Vázquez Betsi Bastidas Estalina Báez‐Ramirez Luz E. Thomas |
| author_sort | Joanna Baricelli |
| collection | DOAJ |
| description | Objective: To describe the antimicrobial activity of β‐defensin‐2 produced in the mammary gland and secreted in human breast milk.
Methods: The peptide production was performed by DNA cloning. β‐defensin‐2 levels were quantified in 61 colostrum samples and 39 mature milk samples from healthy donors, by an indirect enzyme‐linked immunosorbent assay (ELISA). Using halo inhibition assay, this study assessed activity against seven clinical isolates from diarrheal feces of children between 0 and 2 years of age. The activity of β‐defensin‐2 against three opportunistic pathogens that can cause nosocomial infections was determined by microdilution test.
Results: The peptide levels were higher in colostrum (n = 61) than in mature milk samples (n = 39), as follows: median and range, 8.52 (2.6‐16.3) μg/ml versus 0.97 (0.22‐3.78), p < 0.0001; Mann‐Whitney test. The recombinant peptide obtained showed high antimicrobial activity against a broad range of pathogenic bacteria. Its antibacterial activity was demonstrated in a disk containing between 1–4 μg, which produced inhibition zones ranging from 18 to 30 mm against three isolates of Salmonella spp. and four of E. coli. β‐defensin‐2 showed minimum inhibitory concentrations (MICs) of 0.25 μg/mL and 0.5 μg/mL for S. marcescen and P. aeruginosa, respectively, while a higher MIC (4 μg/mL) was obtained against an isolated of multidrug‐resistant strain of A. baumannii.
Conclusions: To the authors’ knowledge, this study is the first to report β‐defensin‐2 levels in Latin American women. The production and the activity of β‐defensin‐2 in breast milk prove its importance as a defense molecule for intestinal health in pediatric patients. |
| first_indexed | 2024-04-12T00:08:40Z |
| format | Article |
| id | doaj.art-d0355f4523e148229f02a4986bd251cc |
| institution | Directory Open Access Journal |
| issn | 2255-5536 |
| language | Portuguese |
| last_indexed | 2024-04-12T00:08:40Z |
| publishDate | 2015-01-01 |
| publisher | Brazilian Society of Pediatrics |
| record_format | Article |
| series | Jornal de Pediatria (Versão em Português) |
| spelling | doaj.art-d0355f4523e148229f02a4986bd251cc2022-12-22T03:56:01ZporBrazilian Society of PediatricsJornal de Pediatria (Versão em Português)2255-55362015-01-01911364310.1016/j.jpedp.2014.05.013β‐defensin‐2 in breast milk displays a broad antimicrobial activity against pathogenic bacteriaJoanna Baricelli0Miguel A. Rocafull1Desiree Vázquez2Betsi Bastidas3Estalina Báez‐Ramirez4Luz E. Thomas5Laboratório de Fisiologia Molecular, Centro de Biofísica e Bioquímica, Instituto Venezolano de Investigaciones Científicas (IVIC), Caracas, VenezuelaLaboratório de Fisiologia Molecular, Centro de Biofísica e Bioquímica, Instituto Venezolano de Investigaciones Científicas (IVIC), Caracas, VenezuelaLaboratório de Fisiologia Molecular, Centro de Biofísica e Bioquímica, Instituto Venezolano de Investigaciones Científicas (IVIC), Caracas, VenezuelaBanco de Leite Humano, Hospital Universitário de Caracas, Caracas, VenezuelaLaboratório de Fisiologia Molecular, Centro de Biofísica e Bioquímica, Instituto Venezolano de Investigaciones Científicas (IVIC), Caracas, VenezuelaLaboratório de Fisiologia Molecular, Centro de Biofísica e Bioquímica, Instituto Venezolano de Investigaciones Científicas (IVIC), Caracas, VenezuelaObjective: To describe the antimicrobial activity of β‐defensin‐2 produced in the mammary gland and secreted in human breast milk. Methods: The peptide production was performed by DNA cloning. β‐defensin‐2 levels were quantified in 61 colostrum samples and 39 mature milk samples from healthy donors, by an indirect enzyme‐linked immunosorbent assay (ELISA). Using halo inhibition assay, this study assessed activity against seven clinical isolates from diarrheal feces of children between 0 and 2 years of age. The activity of β‐defensin‐2 against three opportunistic pathogens that can cause nosocomial infections was determined by microdilution test. Results: The peptide levels were higher in colostrum (n = 61) than in mature milk samples (n = 39), as follows: median and range, 8.52 (2.6‐16.3) μg/ml versus 0.97 (0.22‐3.78), p < 0.0001; Mann‐Whitney test. The recombinant peptide obtained showed high antimicrobial activity against a broad range of pathogenic bacteria. Its antibacterial activity was demonstrated in a disk containing between 1–4 μg, which produced inhibition zones ranging from 18 to 30 mm against three isolates of Salmonella spp. and four of E. coli. β‐defensin‐2 showed minimum inhibitory concentrations (MICs) of 0.25 μg/mL and 0.5 μg/mL for S. marcescen and P. aeruginosa, respectively, while a higher MIC (4 μg/mL) was obtained against an isolated of multidrug‐resistant strain of A. baumannii. Conclusions: To the authors’ knowledge, this study is the first to report β‐defensin‐2 levels in Latin American women. The production and the activity of β‐defensin‐2 in breast milk prove its importance as a defense molecule for intestinal health in pediatric patients.http://www.sciencedirect.com/science/article/pii/S2255553614001554Antimicrobial cationic peptideshBD2LactancyDefensinsAnti‐infective agentsBreast milk |
| spellingShingle | Joanna Baricelli Miguel A. Rocafull Desiree Vázquez Betsi Bastidas Estalina Báez‐Ramirez Luz E. Thomas β‐defensin‐2 in breast milk displays a broad antimicrobial activity against pathogenic bacteria Jornal de Pediatria (Versão em Português) Antimicrobial cationic peptides hBD2 Lactancy Defensins Anti‐infective agents Breast milk |
| title | β‐defensin‐2 in breast milk displays a broad antimicrobial activity against pathogenic bacteria |
| title_full | β‐defensin‐2 in breast milk displays a broad antimicrobial activity against pathogenic bacteria |
| title_fullStr | β‐defensin‐2 in breast milk displays a broad antimicrobial activity against pathogenic bacteria |
| title_full_unstemmed | β‐defensin‐2 in breast milk displays a broad antimicrobial activity against pathogenic bacteria |
| title_short | β‐defensin‐2 in breast milk displays a broad antimicrobial activity against pathogenic bacteria |
| title_sort | β defensin 2 in breast milk displays a broad antimicrobial activity against pathogenic bacteria |
| topic | Antimicrobial cationic peptides hBD2 Lactancy Defensins Anti‐infective agents Breast milk |
| url | http://www.sciencedirect.com/science/article/pii/S2255553614001554 |
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