Walnut Oil Prevents Scopolamine-Induced Memory Dysfunction in a Mouse Model
For thousands of years, it has been widely believed that walnut is a kind of nut that has benefits for the human body. Walnut oil, accounting for about 70% of walnut, mainly consists of polyunsaturated fatty acids. To investigate the effect of walnut oil on memory impairment in mice, scopolamine (3...
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MDPI AG
2020-04-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/25/7/1630 |
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| author | Jianqiao Liao Yifan Nai Li Feng Yimeng Chen Mei Li Huaide Xu |
| author_facet | Jianqiao Liao Yifan Nai Li Feng Yimeng Chen Mei Li Huaide Xu |
| author_sort | Jianqiao Liao |
| collection | DOAJ |
| description | For thousands of years, it has been widely believed that walnut is a kind of nut that has benefits for the human body. Walnut oil, accounting for about 70% of walnut, mainly consists of polyunsaturated fatty acids. To investigate the effect of walnut oil on memory impairment in mice, scopolamine (3 mg/kg body weight/d) was used to establish the animal model during Morris Water Maze (MWM) tests. Walnut oil was administrated orally at 10 mL/kg body weight/d for 8 consecutive weeks. The results showed that walnut oil treatment ameliorated the behavior of the memory-impaired mice in the MWM test. Additionally, walnut oil obviously inhibited acetylcholinesterase activity (1.26 ± 0.12 U/mg prot) (<i>p</i> = 0.013) and increased choline acetyltransferase activity (129.75 ± 6.76 U/mg tissue wet weight) in the brains of scopolamine-treated mice (<i>p</i> = 0.024), suggesting that walnut oil could prevent cholinergic function damage in mice brains. Furthermore, walnut oil remarkably prevented the decrease in total superoxide dismutase activity (93.30 ± 5.50 U/mg prot) (<i>p</i> = 0.006) and glutathione content (110.45 ± 17.70 mg/g prot) (<i>p</i> = 0.047) and the increase of malondialdehyde content (13.79 ± 0.96 nmol/mg prot) (<i>p</i> = 0.001) in the brain of scopolamine-treated mice, indicating that walnut oil could inhibit oxidative stress in the brain of mice. Furthermore, walnut oil prevented histological changes of neurons in hippocampal CA1 and CA3 regions induced by scopolamine. These findings indicate that walnut oil could prevent memory impairment in mice, which might be a potential way for the prevention of memory dysfunctions. |
| first_indexed | 2024-03-10T20:44:17Z |
| format | Article |
| id | doaj.art-d035e8b928d34f278a1705a56d523a5b |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T20:44:17Z |
| publishDate | 2020-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-d035e8b928d34f278a1705a56d523a5b2023-11-19T20:28:27ZengMDPI AGMolecules1420-30492020-04-01257163010.3390/molecules25071630Walnut Oil Prevents Scopolamine-Induced Memory Dysfunction in a Mouse ModelJianqiao Liao0Yifan Nai1Li Feng2Yimeng Chen3Mei Li4Huaide Xu5College of Food Science and Engineering, Northwest A&F University, Yangling 712100, ChinaCollege of Food Science and Engineering, Northwest A&F University, Yangling 712100, ChinaCollege of Food Science and Engineering, Northwest A&F University, Yangling 712100, ChinaCollege of Food Science and Engineering, Northwest A&F University, Yangling 712100, ChinaCollege of Food Science and Engineering, Northwest A&F University, Yangling 712100, ChinaCollege of Food Science and Engineering, Northwest A&F University, Yangling 712100, ChinaFor thousands of years, it has been widely believed that walnut is a kind of nut that has benefits for the human body. Walnut oil, accounting for about 70% of walnut, mainly consists of polyunsaturated fatty acids. To investigate the effect of walnut oil on memory impairment in mice, scopolamine (3 mg/kg body weight/d) was used to establish the animal model during Morris Water Maze (MWM) tests. Walnut oil was administrated orally at 10 mL/kg body weight/d for 8 consecutive weeks. The results showed that walnut oil treatment ameliorated the behavior of the memory-impaired mice in the MWM test. Additionally, walnut oil obviously inhibited acetylcholinesterase activity (1.26 ± 0.12 U/mg prot) (<i>p</i> = 0.013) and increased choline acetyltransferase activity (129.75 ± 6.76 U/mg tissue wet weight) in the brains of scopolamine-treated mice (<i>p</i> = 0.024), suggesting that walnut oil could prevent cholinergic function damage in mice brains. Furthermore, walnut oil remarkably prevented the decrease in total superoxide dismutase activity (93.30 ± 5.50 U/mg prot) (<i>p</i> = 0.006) and glutathione content (110.45 ± 17.70 mg/g prot) (<i>p</i> = 0.047) and the increase of malondialdehyde content (13.79 ± 0.96 nmol/mg prot) (<i>p</i> = 0.001) in the brain of scopolamine-treated mice, indicating that walnut oil could inhibit oxidative stress in the brain of mice. Furthermore, walnut oil prevented histological changes of neurons in hippocampal CA1 and CA3 regions induced by scopolamine. These findings indicate that walnut oil could prevent memory impairment in mice, which might be a potential way for the prevention of memory dysfunctions.https://www.mdpi.com/1420-3049/25/7/1630walnut oilscopolamine-inducedmemory impairmentcholinergic systemoxidative stress |
| spellingShingle | Jianqiao Liao Yifan Nai Li Feng Yimeng Chen Mei Li Huaide Xu Walnut Oil Prevents Scopolamine-Induced Memory Dysfunction in a Mouse Model Molecules walnut oil scopolamine-induced memory impairment cholinergic system oxidative stress |
| title | Walnut Oil Prevents Scopolamine-Induced Memory Dysfunction in a Mouse Model |
| title_full | Walnut Oil Prevents Scopolamine-Induced Memory Dysfunction in a Mouse Model |
| title_fullStr | Walnut Oil Prevents Scopolamine-Induced Memory Dysfunction in a Mouse Model |
| title_full_unstemmed | Walnut Oil Prevents Scopolamine-Induced Memory Dysfunction in a Mouse Model |
| title_short | Walnut Oil Prevents Scopolamine-Induced Memory Dysfunction in a Mouse Model |
| title_sort | walnut oil prevents scopolamine induced memory dysfunction in a mouse model |
| topic | walnut oil scopolamine-induced memory impairment cholinergic system oxidative stress |
| url | https://www.mdpi.com/1420-3049/25/7/1630 |
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