Electrospun, Resorbable, Drug-Eluting, Nanofibrous Membranes Promote Healing of Allograft Tendons
In spite of advances in medical technology, the repair of Achilles tendon ruptures remains challenging. Reconstruction with an autograft tendon provides the advantage of a higher healing rate; nevertheless, the development of donor-site morbidity cannot be ignored. We developed biodegradable, drug-e...
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| Format: | Article |
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MDPI AG
2022-05-01
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| Series: | Membranes |
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| Online Access: | https://www.mdpi.com/2077-0375/12/5/529 |
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| author | Chun-Jui Weng Yu-Chen Wu Ming-Yi Hsu Fu-Pang Chang Shih-Jung Liu |
| author_facet | Chun-Jui Weng Yu-Chen Wu Ming-Yi Hsu Fu-Pang Chang Shih-Jung Liu |
| author_sort | Chun-Jui Weng |
| collection | DOAJ |
| description | In spite of advances in medical technology, the repair of Achilles tendon ruptures remains challenging. Reconstruction with an autograft tendon provides the advantage of a higher healing rate; nevertheless, the development of donor-site morbidity cannot be ignored. We developed biodegradable, drug-eluting, nanofibrous membranes employing an electrospinning technique and evaluated their effectiveness on the healing of allograft tendons. Poly-D-L-lactide-glycolide was used as the polymeric material for the nanofibers, while doxycycline was selected as the drug for delivery. The in vitro and in vivo drug-release profiles were investigated. The biomechanical properties of allografted Achilles tendons repaired using the nanofibrous membranes were tested in euthanized rabbits at 2-, 4-, and 6-week time intervals. Histological examination was performed for the evaluation of tissue reaction and tendon healing. The level of postoperative animal activity was also monitored using an animal behavior cage. The experimental results showed that the degradable nanofibers used as a vehicle could provide sustained release of doxycycline for 42 days after surgery with very low systemic drug concentration. Allograft Achilles tendon reconstruction assisted by drug-loaded nanofibers was associated with better biomechanical properties at 6 weeks post-surgery. In addition, the animals exhibited a better level of activity after surgery. The use of drug-eluting, nanofibrous membranes could enhance healing in Achilles tendon allograft reconstruction surgery. |
| first_indexed | 2024-03-10T03:27:23Z |
| format | Article |
| id | doaj.art-d043b4a2c5084f0ba055239c0f1942ef |
| institution | Directory Open Access Journal |
| issn | 2077-0375 |
| language | English |
| last_indexed | 2024-03-10T03:27:23Z |
| publishDate | 2022-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Membranes |
| spelling | doaj.art-d043b4a2c5084f0ba055239c0f1942ef2023-11-23T12:06:08ZengMDPI AGMembranes2077-03752022-05-0112552910.3390/membranes12050529Electrospun, Resorbable, Drug-Eluting, Nanofibrous Membranes Promote Healing of Allograft TendonsChun-Jui Weng0Yu-Chen Wu1Ming-Yi Hsu2Fu-Pang Chang3Shih-Jung Liu4Department of Orthopedic Surgery, Bone and Joint Research Center, Chang Gung Memorial Hospital-Linkou, Taoyuan City 33305, TaiwanDepartment of Mechanical Engineering, Chang Gung University, Taoyuan City 33302, TaiwanDepartment of Mechanical Engineering, Chang Gung University, Taoyuan City 33302, TaiwanDepartment of Pathology and Laboratory Medicine, Taipei Veterans General Hospital, Taipei 11217, TaiwanDepartment of Orthopedic Surgery, Bone and Joint Research Center, Chang Gung Memorial Hospital-Linkou, Taoyuan City 33305, TaiwanIn spite of advances in medical technology, the repair of Achilles tendon ruptures remains challenging. Reconstruction with an autograft tendon provides the advantage of a higher healing rate; nevertheless, the development of donor-site morbidity cannot be ignored. We developed biodegradable, drug-eluting, nanofibrous membranes employing an electrospinning technique and evaluated their effectiveness on the healing of allograft tendons. Poly-D-L-lactide-glycolide was used as the polymeric material for the nanofibers, while doxycycline was selected as the drug for delivery. The in vitro and in vivo drug-release profiles were investigated. The biomechanical properties of allografted Achilles tendons repaired using the nanofibrous membranes were tested in euthanized rabbits at 2-, 4-, and 6-week time intervals. Histological examination was performed for the evaluation of tissue reaction and tendon healing. The level of postoperative animal activity was also monitored using an animal behavior cage. The experimental results showed that the degradable nanofibers used as a vehicle could provide sustained release of doxycycline for 42 days after surgery with very low systemic drug concentration. Allograft Achilles tendon reconstruction assisted by drug-loaded nanofibers was associated with better biomechanical properties at 6 weeks post-surgery. In addition, the animals exhibited a better level of activity after surgery. The use of drug-eluting, nanofibrous membranes could enhance healing in Achilles tendon allograft reconstruction surgery.https://www.mdpi.com/2077-0375/12/5/529electrospinningdrug-elutingnanofibrous membraneallografttendon repair |
| spellingShingle | Chun-Jui Weng Yu-Chen Wu Ming-Yi Hsu Fu-Pang Chang Shih-Jung Liu Electrospun, Resorbable, Drug-Eluting, Nanofibrous Membranes Promote Healing of Allograft Tendons Membranes electrospinning drug-eluting nanofibrous membrane allograft tendon repair |
| title | Electrospun, Resorbable, Drug-Eluting, Nanofibrous Membranes Promote Healing of Allograft Tendons |
| title_full | Electrospun, Resorbable, Drug-Eluting, Nanofibrous Membranes Promote Healing of Allograft Tendons |
| title_fullStr | Electrospun, Resorbable, Drug-Eluting, Nanofibrous Membranes Promote Healing of Allograft Tendons |
| title_full_unstemmed | Electrospun, Resorbable, Drug-Eluting, Nanofibrous Membranes Promote Healing of Allograft Tendons |
| title_short | Electrospun, Resorbable, Drug-Eluting, Nanofibrous Membranes Promote Healing of Allograft Tendons |
| title_sort | electrospun resorbable drug eluting nanofibrous membranes promote healing of allograft tendons |
| topic | electrospinning drug-eluting nanofibrous membrane allograft tendon repair |
| url | https://www.mdpi.com/2077-0375/12/5/529 |
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