Investigating the Mechanical Characteristics of Bone-Metal Implant Interface Using in situ Synchrotron Tomographic Imaging
Long-term stability of endosseous implants depends on successful bone formation, ingrowth and adaptation to the implant. Specifically, it will define the mechanical properties of the newly formed bone-implant interface. 3D imaging during mechanical loading tests (in situ loading) can improve the und...
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Frontiers Media S.A.
2019-01-01
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| Series: | Frontiers in Bioengineering and Biotechnology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fbioe.2018.00208/full |
| _version_ | 1831716329997991936 |
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| author | Sophie Le Cann Erika Tudisco Mikael J. Turunen Alessandra Patera Rajmund Mokso Magnus Tägil Ola Belfrage Stephen A. Hall Hanna Isaksson Hanna Isaksson |
| author_facet | Sophie Le Cann Erika Tudisco Mikael J. Turunen Alessandra Patera Rajmund Mokso Magnus Tägil Ola Belfrage Stephen A. Hall Hanna Isaksson Hanna Isaksson |
| author_sort | Sophie Le Cann |
| collection | DOAJ |
| description | Long-term stability of endosseous implants depends on successful bone formation, ingrowth and adaptation to the implant. Specifically, it will define the mechanical properties of the newly formed bone-implant interface. 3D imaging during mechanical loading tests (in situ loading) can improve the understanding of the local processes leading to bone damage and failure. In this study, titanium screws were implanted into rat tibiae and were allowed to integrate for 4 weeks with or without the addition of the growth factor Bone Morphogenetic Protein and the bisphosphonate Zoledronic Acid. Samples were subjected to in situ pullout using high-resolution synchrotron x-ray tomography at the Tomcat beamline (SLS, PSI, Switzerland) at 30 keV with 25 ms exposure time, resulting in a total acquisition time of 45 s per scan, with a 3.6 μm isotropic voxel size. Using a custom-made loading device positioned inside the beamline, screws were pulled out with 0.05 mm increment, acquiring multiple scans until rupture of the sample. The in situ loading protocol was adapted to ensure short imaging time, which enabled multiple samples to be tested with short loading steps, while keeping the total testing time low and reducing dose deposition. Higher trabecular bone content was quantified in the surrounding of the screw in the treated groups, which correlated with increased mechanical strength and stiffness. Differences in screw implantation, such as contact between threads and cortex as well as minor tilt of the screw were also correlated to the mechanical parameters. In situ loading enabled the investigation of crack propagation during the pullout, highlighting the mechanical behavior of the interface. Three typical crack types were observed: (1) rupture at the interface of trabecular and cortical bone tissues, close to the screw, (2) large crack inside the cortex connected to the implant, and (3) first failure away from the screw with cracks propagating toward the screw-bone interface. Mechanical properties of in vivo integrated bone-metal screws rely on a combination of multiple parameters that are difficult to identify and separate one from the other. |
| first_indexed | 2024-12-21T00:38:04Z |
| format | Article |
| id | doaj.art-d04c493708ed44d0b8863cb1bb7c31d2 |
| institution | Directory Open Access Journal |
| issn | 2296-4185 |
| language | English |
| last_indexed | 2024-12-21T00:38:04Z |
| publishDate | 2019-01-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj.art-d04c493708ed44d0b8863cb1bb7c31d22022-12-21T19:21:45ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852019-01-01610.3389/fbioe.2018.00208424351Investigating the Mechanical Characteristics of Bone-Metal Implant Interface Using in situ Synchrotron Tomographic ImagingSophie Le Cann0Erika Tudisco1Mikael J. Turunen2Alessandra Patera3Rajmund Mokso4Magnus Tägil5Ola Belfrage6Stephen A. Hall7Hanna Isaksson8Hanna Isaksson9Department of Biomedical Engineering, Lund University, Lund, SwedenDivision of Geotechnical Engineering, Lund University, Lund, SwedenDepartment of Applied Physics, University of Eastern Finland, Kuopio, FinlandSwiss Light Source, Paul Scherrer Institute, Villigen, SwitzerlandMax IV laboratory, Lund University, Lund, SwedenDepartment of Orthopaedics, Clinical Sciences, Lund University, Lund, SwedenDepartment of Orthopaedics, Clinical Sciences, Lund University, Lund, SwedenDivision of Solid Mechanics, Lund University, Lund, SwedenDepartment of Biomedical Engineering, Lund University, Lund, SwedenDepartment of Orthopaedics, Clinical Sciences, Lund University, Lund, SwedenLong-term stability of endosseous implants depends on successful bone formation, ingrowth and adaptation to the implant. Specifically, it will define the mechanical properties of the newly formed bone-implant interface. 3D imaging during mechanical loading tests (in situ loading) can improve the understanding of the local processes leading to bone damage and failure. In this study, titanium screws were implanted into rat tibiae and were allowed to integrate for 4 weeks with or without the addition of the growth factor Bone Morphogenetic Protein and the bisphosphonate Zoledronic Acid. Samples were subjected to in situ pullout using high-resolution synchrotron x-ray tomography at the Tomcat beamline (SLS, PSI, Switzerland) at 30 keV with 25 ms exposure time, resulting in a total acquisition time of 45 s per scan, with a 3.6 μm isotropic voxel size. Using a custom-made loading device positioned inside the beamline, screws were pulled out with 0.05 mm increment, acquiring multiple scans until rupture of the sample. The in situ loading protocol was adapted to ensure short imaging time, which enabled multiple samples to be tested with short loading steps, while keeping the total testing time low and reducing dose deposition. Higher trabecular bone content was quantified in the surrounding of the screw in the treated groups, which correlated with increased mechanical strength and stiffness. Differences in screw implantation, such as contact between threads and cortex as well as minor tilt of the screw were also correlated to the mechanical parameters. In situ loading enabled the investigation of crack propagation during the pullout, highlighting the mechanical behavior of the interface. Three typical crack types were observed: (1) rupture at the interface of trabecular and cortical bone tissues, close to the screw, (2) large crack inside the cortex connected to the implant, and (3) first failure away from the screw with cracks propagating toward the screw-bone interface. Mechanical properties of in vivo integrated bone-metal screws rely on a combination of multiple parameters that are difficult to identify and separate one from the other.https://www.frontiersin.org/article/10.3389/fbioe.2018.00208/fullX-ray tomographybonemetallic screwin situ loadingsynchrotron |
| spellingShingle | Sophie Le Cann Erika Tudisco Mikael J. Turunen Alessandra Patera Rajmund Mokso Magnus Tägil Ola Belfrage Stephen A. Hall Hanna Isaksson Hanna Isaksson Investigating the Mechanical Characteristics of Bone-Metal Implant Interface Using in situ Synchrotron Tomographic Imaging Frontiers in Bioengineering and Biotechnology X-ray tomography bone metallic screw in situ loading synchrotron |
| title | Investigating the Mechanical Characteristics of Bone-Metal Implant Interface Using in situ Synchrotron Tomographic Imaging |
| title_full | Investigating the Mechanical Characteristics of Bone-Metal Implant Interface Using in situ Synchrotron Tomographic Imaging |
| title_fullStr | Investigating the Mechanical Characteristics of Bone-Metal Implant Interface Using in situ Synchrotron Tomographic Imaging |
| title_full_unstemmed | Investigating the Mechanical Characteristics of Bone-Metal Implant Interface Using in situ Synchrotron Tomographic Imaging |
| title_short | Investigating the Mechanical Characteristics of Bone-Metal Implant Interface Using in situ Synchrotron Tomographic Imaging |
| title_sort | investigating the mechanical characteristics of bone metal implant interface using in situ synchrotron tomographic imaging |
| topic | X-ray tomography bone metallic screw in situ loading synchrotron |
| url | https://www.frontiersin.org/article/10.3389/fbioe.2018.00208/full |
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