SCRIB Is Involved in the Progression of Ovarian Carcinomas in Association with the Factors Linked to Epithelial-to-Mesenchymal Transition and Predicts Shorter Survival of Diagnosed Patients
SCRIB is a polarity protein important in maintaining cell junctions. However, recent reports have raised the possibility that SCRIB might have a role in human cancers. Thus, this study evaluated the roles of SCRIB in ovarian cancers. In 102 human ovarian carcinomas, nuclear expression of SCRIB predi...
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| Format: | Article |
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MDPI AG
2021-03-01
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| Series: | Biomolecules |
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| Online Access: | https://www.mdpi.com/2218-273X/11/3/405 |
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| author | Usama Khamis Hussein Asmaa Gamal Ahmed Won Ku Choi Kyoung Min Kim See-Hyoung Park Ho Sung Park Sang Jae Noh Ho Lee Myoung Ja Chung Woo Sung Moon Myoung Jae Kang Dong Hyu Cho Kyu Yun Jang |
| author_facet | Usama Khamis Hussein Asmaa Gamal Ahmed Won Ku Choi Kyoung Min Kim See-Hyoung Park Ho Sung Park Sang Jae Noh Ho Lee Myoung Ja Chung Woo Sung Moon Myoung Jae Kang Dong Hyu Cho Kyu Yun Jang |
| author_sort | Usama Khamis Hussein |
| collection | DOAJ |
| description | SCRIB is a polarity protein important in maintaining cell junctions. However, recent reports have raised the possibility that SCRIB might have a role in human cancers. Thus, this study evaluated the roles of SCRIB in ovarian cancers. In 102 human ovarian carcinomas, nuclear expression of SCRIB predicted shorter survival of ovarian carcinoma patients, especially in the patients who received post-operative chemotherapy. In SKOV3 and SNU119 ovarian cancer cells, overexpression of SCRIB stimulated the proliferation and invasion of cells. Knockout of SCRIB inhibited in vivo tumor growth of SKOV3 cells and overexpression of SCRIB promoted tumor growth. Overexpression of SCRIB stimulated epithelial-to-mesenchymal transition by increasing the expression of N-cadherin, snail, TGF-β1, and smad2/3, and decreasing the expression of E-cadherin; the converse was observed with inhibition of SCRIB. In conclusion, this study presents the nuclear expression of SCRIB as a prognostic marker of ovarian carcinomas and suggests that SCRIB is involved in the progression of ovarian carcinomas by stimulating proliferation and epithelial-to-mesenchymal transition-related invasiveness. |
| first_indexed | 2024-03-10T13:24:36Z |
| format | Article |
| id | doaj.art-d04c588cbddb44cfaa3864b1af2b21cc |
| institution | Directory Open Access Journal |
| issn | 2218-273X |
| language | English |
| last_indexed | 2024-03-10T13:24:36Z |
| publishDate | 2021-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomolecules |
| spelling | doaj.art-d04c588cbddb44cfaa3864b1af2b21cc2023-11-21T09:46:23ZengMDPI AGBiomolecules2218-273X2021-03-0111340510.3390/biom11030405SCRIB Is Involved in the Progression of Ovarian Carcinomas in Association with the Factors Linked to Epithelial-to-Mesenchymal Transition and Predicts Shorter Survival of Diagnosed PatientsUsama Khamis Hussein0Asmaa Gamal Ahmed1Won Ku Choi2Kyoung Min Kim3See-Hyoung Park4Ho Sung Park5Sang Jae Noh6Ho Lee7Myoung Ja Chung8Woo Sung Moon9Myoung Jae Kang10Dong Hyu Cho11Kyu Yun Jang12Department of Pathology, Jeonbuk National University Medical School, Jeonju 54896, KoreaDepartment of Pathology, Jeonbuk National University Medical School, Jeonju 54896, KoreaResearch Institute of Clinical Medicine of Jeonbuk National University-Biomedical Research Institute of Jeonbuk National University Hospital, Jeonju 54907, KoreaDepartment of Pathology, Jeonbuk National University Medical School, Jeonju 54896, KoreaDepartment of Bio and Chemical Engineering, Hongik University, Sejong 30016, KoreaDepartment of Pathology, Jeonbuk National University Medical School, Jeonju 54896, KoreaDepartment of Forensic Medicine, Jeonbuk National University Medical School, Jeonju 54896, KoreaDepartment of Forensic Medicine, Jeonbuk National University Medical School, Jeonju 54896, KoreaDepartment of Pathology, Jeonbuk National University Medical School, Jeonju 54896, KoreaDepartment of Pathology, Jeonbuk National University Medical School, Jeonju 54896, KoreaDepartment of Pathology, Jeonbuk National University Medical School, Jeonju 54896, KoreaResearch Institute of Clinical Medicine of Jeonbuk National University-Biomedical Research Institute of Jeonbuk National University Hospital, Jeonju 54907, KoreaDepartment of Pathology, Jeonbuk National University Medical School, Jeonju 54896, KoreaSCRIB is a polarity protein important in maintaining cell junctions. However, recent reports have raised the possibility that SCRIB might have a role in human cancers. Thus, this study evaluated the roles of SCRIB in ovarian cancers. In 102 human ovarian carcinomas, nuclear expression of SCRIB predicted shorter survival of ovarian carcinoma patients, especially in the patients who received post-operative chemotherapy. In SKOV3 and SNU119 ovarian cancer cells, overexpression of SCRIB stimulated the proliferation and invasion of cells. Knockout of SCRIB inhibited in vivo tumor growth of SKOV3 cells and overexpression of SCRIB promoted tumor growth. Overexpression of SCRIB stimulated epithelial-to-mesenchymal transition by increasing the expression of N-cadherin, snail, TGF-β1, and smad2/3, and decreasing the expression of E-cadherin; the converse was observed with inhibition of SCRIB. In conclusion, this study presents the nuclear expression of SCRIB as a prognostic marker of ovarian carcinomas and suggests that SCRIB is involved in the progression of ovarian carcinomas by stimulating proliferation and epithelial-to-mesenchymal transition-related invasiveness.https://www.mdpi.com/2218-273X/11/3/405ovarycancerSCRIBepithelial-to-mesenchymal transitionprognosis |
| spellingShingle | Usama Khamis Hussein Asmaa Gamal Ahmed Won Ku Choi Kyoung Min Kim See-Hyoung Park Ho Sung Park Sang Jae Noh Ho Lee Myoung Ja Chung Woo Sung Moon Myoung Jae Kang Dong Hyu Cho Kyu Yun Jang SCRIB Is Involved in the Progression of Ovarian Carcinomas in Association with the Factors Linked to Epithelial-to-Mesenchymal Transition and Predicts Shorter Survival of Diagnosed Patients Biomolecules ovary cancer SCRIB epithelial-to-mesenchymal transition prognosis |
| title | SCRIB Is Involved in the Progression of Ovarian Carcinomas in Association with the Factors Linked to Epithelial-to-Mesenchymal Transition and Predicts Shorter Survival of Diagnosed Patients |
| title_full | SCRIB Is Involved in the Progression of Ovarian Carcinomas in Association with the Factors Linked to Epithelial-to-Mesenchymal Transition and Predicts Shorter Survival of Diagnosed Patients |
| title_fullStr | SCRIB Is Involved in the Progression of Ovarian Carcinomas in Association with the Factors Linked to Epithelial-to-Mesenchymal Transition and Predicts Shorter Survival of Diagnosed Patients |
| title_full_unstemmed | SCRIB Is Involved in the Progression of Ovarian Carcinomas in Association with the Factors Linked to Epithelial-to-Mesenchymal Transition and Predicts Shorter Survival of Diagnosed Patients |
| title_short | SCRIB Is Involved in the Progression of Ovarian Carcinomas in Association with the Factors Linked to Epithelial-to-Mesenchymal Transition and Predicts Shorter Survival of Diagnosed Patients |
| title_sort | scrib is involved in the progression of ovarian carcinomas in association with the factors linked to epithelial to mesenchymal transition and predicts shorter survival of diagnosed patients |
| topic | ovary cancer SCRIB epithelial-to-mesenchymal transition prognosis |
| url | https://www.mdpi.com/2218-273X/11/3/405 |
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