A case-controlled validation study of a blood-based seven-gene biomarker panel for colorectal cancer in Malaysia
<p>Abstract</p> <p>Background</p> <p>Colorectal cancer (CRC) screening is key to CRC prevention and mortality reduction, but patient compliance with CRC screening is low. We previously reported a blood-based test for CRC that utilizes a seven-gene panel of biomarkers. T...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2010-09-01
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| Series: | Journal of Experimental & Clinical Cancer Research |
| Online Access: | http://www.jeccr.com/content/29/1/128 |
| _version_ | 1818541677601619968 |
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| author | Yip Kok-Thye Das Prashanta K Suria David Lim Chun-Ren Ng Guey-Hooi Liew Choong-Chin |
| author_facet | Yip Kok-Thye Das Prashanta K Suria David Lim Chun-Ren Ng Guey-Hooi Liew Choong-Chin |
| author_sort | Yip Kok-Thye |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Colorectal cancer (CRC) screening is key to CRC prevention and mortality reduction, but patient compliance with CRC screening is low. We previously reported a blood-based test for CRC that utilizes a seven-gene panel of biomarkers. The test is currently utilized clinically in North America for CRC risk stratification in the average-risk North American population in order to improve screening compliance and to enhance clinical decision making.</p> <p>Methods</p> <p>In this study, conducted in Malaysia, we evaluated the seven-gene biomarker panel validated in a North American population using blood samples collected from local patients. The panel employs quantitative RT-PCR (qRT-PCR) to analyze gene expression of the seven biomarkers (ANXA3, CLEC4D, TNFAIP6, LMNB1, PRRG4, VNN1 and IL2RB) that are differentially expressed in CRC patients as compared with controls. Blood samples from 210 patients (99 CRC and 111 controls) were collected, and total blood RNA was isolated and subjected to quantitative RT-PCR and data analysis.</p> <p>Results</p> <p>The logistic regression analysis of seven-gene panel has an area under the curve (AUC) of 0.76 (95% confidence interval: 0.70 to 0.82), 77% specificity, 61% sensitivity and 70% accuracy, comparable to the data obtained from the North American investigation of the same biomarker panel.</p> <p>Conclusions</p> <p>Our results independently confirm the results of the study conducted in North America and demonstrate the ability of the seven biomarker panel to discriminate CRC from controls in blood samples drawn from a Malaysian population.</p> |
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| format | Article |
| id | doaj.art-d04c84d6e3ba41d78a3575672d0ff6bc |
| institution | Directory Open Access Journal |
| issn | 1756-9966 |
| language | English |
| last_indexed | 2024-12-11T22:12:21Z |
| publishDate | 2010-09-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Experimental & Clinical Cancer Research |
| spelling | doaj.art-d04c84d6e3ba41d78a3575672d0ff6bc2022-12-22T00:48:44ZengBMCJournal of Experimental & Clinical Cancer Research1756-99662010-09-0129112810.1186/1756-9966-29-128A case-controlled validation study of a blood-based seven-gene biomarker panel for colorectal cancer in MalaysiaYip Kok-ThyeDas Prashanta KSuria DavidLim Chun-RenNg Guey-HooiLiew Choong-Chin<p>Abstract</p> <p>Background</p> <p>Colorectal cancer (CRC) screening is key to CRC prevention and mortality reduction, but patient compliance with CRC screening is low. We previously reported a blood-based test for CRC that utilizes a seven-gene panel of biomarkers. The test is currently utilized clinically in North America for CRC risk stratification in the average-risk North American population in order to improve screening compliance and to enhance clinical decision making.</p> <p>Methods</p> <p>In this study, conducted in Malaysia, we evaluated the seven-gene biomarker panel validated in a North American population using blood samples collected from local patients. The panel employs quantitative RT-PCR (qRT-PCR) to analyze gene expression of the seven biomarkers (ANXA3, CLEC4D, TNFAIP6, LMNB1, PRRG4, VNN1 and IL2RB) that are differentially expressed in CRC patients as compared with controls. Blood samples from 210 patients (99 CRC and 111 controls) were collected, and total blood RNA was isolated and subjected to quantitative RT-PCR and data analysis.</p> <p>Results</p> <p>The logistic regression analysis of seven-gene panel has an area under the curve (AUC) of 0.76 (95% confidence interval: 0.70 to 0.82), 77% specificity, 61% sensitivity and 70% accuracy, comparable to the data obtained from the North American investigation of the same biomarker panel.</p> <p>Conclusions</p> <p>Our results independently confirm the results of the study conducted in North America and demonstrate the ability of the seven biomarker panel to discriminate CRC from controls in blood samples drawn from a Malaysian population.</p>http://www.jeccr.com/content/29/1/128 |
| spellingShingle | Yip Kok-Thye Das Prashanta K Suria David Lim Chun-Ren Ng Guey-Hooi Liew Choong-Chin A case-controlled validation study of a blood-based seven-gene biomarker panel for colorectal cancer in Malaysia Journal of Experimental & Clinical Cancer Research |
| title | A case-controlled validation study of a blood-based seven-gene biomarker panel for colorectal cancer in Malaysia |
| title_full | A case-controlled validation study of a blood-based seven-gene biomarker panel for colorectal cancer in Malaysia |
| title_fullStr | A case-controlled validation study of a blood-based seven-gene biomarker panel for colorectal cancer in Malaysia |
| title_full_unstemmed | A case-controlled validation study of a blood-based seven-gene biomarker panel for colorectal cancer in Malaysia |
| title_short | A case-controlled validation study of a blood-based seven-gene biomarker panel for colorectal cancer in Malaysia |
| title_sort | case controlled validation study of a blood based seven gene biomarker panel for colorectal cancer in malaysia |
| url | http://www.jeccr.com/content/29/1/128 |
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