Divergence in Glyphosate Susceptibility between <i>Steinchisma laxum</i> Populations Involves a Pro106Ser Mutation
The characterization of the mechanisms conferring resistance to herbicides in weeds is essential for developing effective management programs. This study was focused on characterizing the resistance level and the main mechanisms that confer resistance to glyphosate in a resistant (R) <i>Steinc...
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MDPI AG
2023-09-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/12/18/3315 |
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| author | Veronica Hoyos Guido Plaza Candelario Palma-Bautista Jose G. Vázquez-García José Alfredo Dominguez-Valenzuela Ricardo Alcántara-de la Cruz Rafael De Prado |
| author_facet | Veronica Hoyos Guido Plaza Candelario Palma-Bautista Jose G. Vázquez-García José Alfredo Dominguez-Valenzuela Ricardo Alcántara-de la Cruz Rafael De Prado |
| author_sort | Veronica Hoyos |
| collection | DOAJ |
| description | The characterization of the mechanisms conferring resistance to herbicides in weeds is essential for developing effective management programs. This study was focused on characterizing the resistance level and the main mechanisms that confer resistance to glyphosate in a resistant (R) <i>Steinchisma laxum</i> population collected in a Colombian rice field in 2020. The R population exhibited 11.2 times higher resistance compared to a susceptible (S) population. Non-target site resistance (NTSR) mechanisms that reduced absorption and impaired translocation and glyphosate metabolism were not involved in the resistance to glyphosate in the R population. Evaluating the target site resistance mechanisms by means of enzymatic activity assays and <i>EPSPS</i> (5-enolpyruvylshikimate-3-phosphate synthase) gene sequencing, the mutation Pro106Ser was found in R plants of <i>S. laxum</i>. These findings are crucial for managing the spread of <i>S. laxum</i> resistance in Colombia. To effectively control <i>S. laxum</i> in the future, it is imperative that farmers use herbicides with different mechanisms of action in addition to glyphosate and adopt Integrate Management Programs to control weeds in rice fields of the central valleys of Colombia. |
| first_indexed | 2024-03-10T22:12:46Z |
| format | Article |
| id | doaj.art-d04fa5a57a704c12bd551f40b1cf8bde |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-10T22:12:46Z |
| publishDate | 2023-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-d04fa5a57a704c12bd551f40b1cf8bde2023-11-19T12:33:09ZengMDPI AGPlants2223-77472023-09-011218331510.3390/plants12183315Divergence in Glyphosate Susceptibility between <i>Steinchisma laxum</i> Populations Involves a Pro106Ser MutationVeronica Hoyos0Guido Plaza1Candelario Palma-Bautista2Jose G. Vázquez-García3José Alfredo Dominguez-Valenzuela4Ricardo Alcántara-de la Cruz5Rafael De Prado6Departamento de Ciencias Biológicas, Universidad Nacional de Colombia, Palmira 763533, ColombiaDepartamento de Agronomía, Universidad Nacional de Colombia, Bogotá 111321, ColombiaDepartamento de Parasitología Agrícola, Universidad Autónoma Chapingo, Texcoco 56230, MexicoAgroforestry and Plant Biochemistry, Proteomics and Systems Biology, Department of Biochemistry and Molecular Biology, University of Cordoba, 14014 Cordoba, SpainDepartamento de Parasitología Agrícola, Universidad Autónoma Chapingo, Texcoco 56230, MexicoDepartamento de Química, Universidade Federal de São Carlos, São Carlos 13565-905, BrazilAgroforestry and Plant Biochemistry, Proteomics and Systems Biology, Department of Biochemistry and Molecular Biology, University of Cordoba, 14014 Cordoba, SpainThe characterization of the mechanisms conferring resistance to herbicides in weeds is essential for developing effective management programs. This study was focused on characterizing the resistance level and the main mechanisms that confer resistance to glyphosate in a resistant (R) <i>Steinchisma laxum</i> population collected in a Colombian rice field in 2020. The R population exhibited 11.2 times higher resistance compared to a susceptible (S) population. Non-target site resistance (NTSR) mechanisms that reduced absorption and impaired translocation and glyphosate metabolism were not involved in the resistance to glyphosate in the R population. Evaluating the target site resistance mechanisms by means of enzymatic activity assays and <i>EPSPS</i> (5-enolpyruvylshikimate-3-phosphate synthase) gene sequencing, the mutation Pro106Ser was found in R plants of <i>S. laxum</i>. These findings are crucial for managing the spread of <i>S. laxum</i> resistance in Colombia. To effectively control <i>S. laxum</i> in the future, it is imperative that farmers use herbicides with different mechanisms of action in addition to glyphosate and adopt Integrate Management Programs to control weeds in rice fields of the central valleys of Colombia.https://www.mdpi.com/2223-7747/12/18/3315<i>EPSPS</i> genelax panicgrassN-(phosphonomethyl) glycineTSR mechanismsrice cultivation |
| spellingShingle | Veronica Hoyos Guido Plaza Candelario Palma-Bautista Jose G. Vázquez-García José Alfredo Dominguez-Valenzuela Ricardo Alcántara-de la Cruz Rafael De Prado Divergence in Glyphosate Susceptibility between <i>Steinchisma laxum</i> Populations Involves a Pro106Ser Mutation Plants <i>EPSPS</i> gene lax panicgrass N-(phosphonomethyl) glycine TSR mechanisms rice cultivation |
| title | Divergence in Glyphosate Susceptibility between <i>Steinchisma laxum</i> Populations Involves a Pro106Ser Mutation |
| title_full | Divergence in Glyphosate Susceptibility between <i>Steinchisma laxum</i> Populations Involves a Pro106Ser Mutation |
| title_fullStr | Divergence in Glyphosate Susceptibility between <i>Steinchisma laxum</i> Populations Involves a Pro106Ser Mutation |
| title_full_unstemmed | Divergence in Glyphosate Susceptibility between <i>Steinchisma laxum</i> Populations Involves a Pro106Ser Mutation |
| title_short | Divergence in Glyphosate Susceptibility between <i>Steinchisma laxum</i> Populations Involves a Pro106Ser Mutation |
| title_sort | divergence in glyphosate susceptibility between i steinchisma laxum i populations involves a pro106ser mutation |
| topic | <i>EPSPS</i> gene lax panicgrass N-(phosphonomethyl) glycine TSR mechanisms rice cultivation |
| url | https://www.mdpi.com/2223-7747/12/18/3315 |
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