Protective effects of selenium on acrylamide-induced neurotoxicity and hepatotoxicity in rats
Objective(s): Acrylamide (ACR), has wide uses in different industries. ACR induced several toxicities including neurotoxicity and hepatotoxicity. The probable protective effects of selenium on ACR-induced neurotoxicity and hepatotoxicity in rats were evaluated.Materials and Methods: Male Wistar rats...
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| Format: | Article |
| Language: | English |
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Mashhad University of Medical Sciences
2021-08-01
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| Series: | Iranian Journal of Basic Medical Sciences |
| Subjects: | |
| Online Access: | https://ijbms.mums.ac.ir/article_18460_8e6ad296f1c4fdadb1b86d10c0bd571e.pdf |
| _version_ | 1818823431579238400 |
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| author | Mahboobeh Ghasemzadeh Rahbardar Hadi Cheraghi Farmed Hossein Hosseinzadeh Soghra Mehri |
| author_facet | Mahboobeh Ghasemzadeh Rahbardar Hadi Cheraghi Farmed Hossein Hosseinzadeh Soghra Mehri |
| author_sort | Mahboobeh Ghasemzadeh Rahbardar |
| collection | DOAJ |
| description | Objective(s): Acrylamide (ACR), has wide uses in different industries. ACR induced several toxicities including neurotoxicity and hepatotoxicity. The probable protective effects of selenium on ACR-induced neurotoxicity and hepatotoxicity in rats were evaluated.Materials and Methods: Male Wistar rats were studied for 11 days in 8 groups: 1. Control, 2. ACR (50 mg/kg, IP), 3-5. ACR+ selenium (0.2, 0.4, 0.6 mg/kg, IP), 6. ACR+ the most effective dose of selenium (0.6 mg/kg, IP) three days after ACR administration, 7. ACR+ vitamin E (200 mg/kg IP, every other day) 8. Selenium (0.6 mg/kg IP). Finally, behavioral tests were done. The levels of malondialdehyde (MDA), glutathione (GSH), Bcl-2, Bax and caspase 3 proteins in liver and cerebral cortex tissues were measured. Also, the amount of albumin, total protein, alanine transaminase (ALT) and aspartate transaminase (AST) enzymes were determined in serum. Results: ACR caused the severe motor impairment, increased MDA level and decreased GSH content, enhanced Bax/Bcl-2 ratio and caspase 3 proteins in brain and liver tissues. Besides, the level of AST was elevated while the total serum protein and albumin levels were decreased. Administration of selenium (0.6 mg/kg) (from the first day of the experiment and the third day) significantly recovered locomotor disorders, increased GSH content, and reduced MDA level. Also, selenium decreased Bax/Bcl-2 ratio and caspase 3 levels in brain and liver tissues.Conclusion: The oxidative stress and apoptosis pathways have important roles in neurotoxicity and hepatotoxicity of ACR. Selenium significantly reduced ACR-induced toxicity through inhibition of oxidative stress and apoptosis. |
| first_indexed | 2024-12-18T23:39:52Z |
| format | Article |
| id | doaj.art-d052154557f4483d8dd28c37a84e42ca |
| institution | Directory Open Access Journal |
| issn | 2008-3866 2008-3874 |
| language | English |
| last_indexed | 2024-12-18T23:39:52Z |
| publishDate | 2021-08-01 |
| publisher | Mashhad University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Basic Medical Sciences |
| spelling | doaj.art-d052154557f4483d8dd28c37a84e42ca2022-12-21T20:47:25ZengMashhad University of Medical SciencesIranian Journal of Basic Medical Sciences2008-38662008-38742021-08-012481041104910.22038/ijbms.2021.55009.1233118460Protective effects of selenium on acrylamide-induced neurotoxicity and hepatotoxicity in ratsMahboobeh Ghasemzadeh Rahbardar0Hadi Cheraghi Farmed1Hossein Hosseinzadeh2Soghra Mehri3Pharmaceutical Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, IranSchool of Pharmacy, Mashhad University of Medical Sciences, Mashhad, IranPharmaceutical Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, IranPharmaceutical Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, IranObjective(s): Acrylamide (ACR), has wide uses in different industries. ACR induced several toxicities including neurotoxicity and hepatotoxicity. The probable protective effects of selenium on ACR-induced neurotoxicity and hepatotoxicity in rats were evaluated.Materials and Methods: Male Wistar rats were studied for 11 days in 8 groups: 1. Control, 2. ACR (50 mg/kg, IP), 3-5. ACR+ selenium (0.2, 0.4, 0.6 mg/kg, IP), 6. ACR+ the most effective dose of selenium (0.6 mg/kg, IP) three days after ACR administration, 7. ACR+ vitamin E (200 mg/kg IP, every other day) 8. Selenium (0.6 mg/kg IP). Finally, behavioral tests were done. The levels of malondialdehyde (MDA), glutathione (GSH), Bcl-2, Bax and caspase 3 proteins in liver and cerebral cortex tissues were measured. Also, the amount of albumin, total protein, alanine transaminase (ALT) and aspartate transaminase (AST) enzymes were determined in serum. Results: ACR caused the severe motor impairment, increased MDA level and decreased GSH content, enhanced Bax/Bcl-2 ratio and caspase 3 proteins in brain and liver tissues. Besides, the level of AST was elevated while the total serum protein and albumin levels were decreased. Administration of selenium (0.6 mg/kg) (from the first day of the experiment and the third day) significantly recovered locomotor disorders, increased GSH content, and reduced MDA level. Also, selenium decreased Bax/Bcl-2 ratio and caspase 3 levels in brain and liver tissues.Conclusion: The oxidative stress and apoptosis pathways have important roles in neurotoxicity and hepatotoxicity of ACR. Selenium significantly reduced ACR-induced toxicity through inhibition of oxidative stress and apoptosis.https://ijbms.mums.ac.ir/article_18460_8e6ad296f1c4fdadb1b86d10c0bd571e.pdfacrylamideapoptosishepatotoxicityneurotoxicityoxidative stressselenium |
| spellingShingle | Mahboobeh Ghasemzadeh Rahbardar Hadi Cheraghi Farmed Hossein Hosseinzadeh Soghra Mehri Protective effects of selenium on acrylamide-induced neurotoxicity and hepatotoxicity in rats Iranian Journal of Basic Medical Sciences acrylamide apoptosis hepatotoxicity neurotoxicity oxidative stress selenium |
| title | Protective effects of selenium on acrylamide-induced neurotoxicity and hepatotoxicity in rats |
| title_full | Protective effects of selenium on acrylamide-induced neurotoxicity and hepatotoxicity in rats |
| title_fullStr | Protective effects of selenium on acrylamide-induced neurotoxicity and hepatotoxicity in rats |
| title_full_unstemmed | Protective effects of selenium on acrylamide-induced neurotoxicity and hepatotoxicity in rats |
| title_short | Protective effects of selenium on acrylamide-induced neurotoxicity and hepatotoxicity in rats |
| title_sort | protective effects of selenium on acrylamide induced neurotoxicity and hepatotoxicity in rats |
| topic | acrylamide apoptosis hepatotoxicity neurotoxicity oxidative stress selenium |
| url | https://ijbms.mums.ac.ir/article_18460_8e6ad296f1c4fdadb1b86d10c0bd571e.pdf |
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