Effect of fluoride gels with nano-sized sodium trimetaphosphate on the in vitro remineralization of caries lesions
Abstract Objective To evaluate the effects of fluoride (F) gels supplemented with micrometric or nano-sized sodium trimetaphosphate (TMPmicro and TMPnano, respectively) on the in vitro remineralization of caries-like lesions. Methodology Bovine enamel subsurface lesions (n=168) were selected acc...
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| Format: | Article |
| Language: | English |
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University of São Paulo
2023-06-01
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| Series: | Journal of Applied Oral Science |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-77572023000100430&tlng=en |
| _version_ | 1797791409437147136 |
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| author | Mariana Emi NAGATA Alberto Carlos Botazzo DELBEM Liliana Carolina BÁEZ-QUINTERO Marcelle DANELON Caio SAMPAIO Douglas Roberto MONTEIRO Annette WIEGAND Juliano Pelim PESSAN |
| author_facet | Mariana Emi NAGATA Alberto Carlos Botazzo DELBEM Liliana Carolina BÁEZ-QUINTERO Marcelle DANELON Caio SAMPAIO Douglas Roberto MONTEIRO Annette WIEGAND Juliano Pelim PESSAN |
| author_sort | Mariana Emi NAGATA |
| collection | DOAJ |
| description | Abstract Objective To evaluate the effects of fluoride (F) gels supplemented with micrometric or nano-sized sodium trimetaphosphate (TMPmicro and TMPnano, respectively) on the in vitro remineralization of caries-like lesions. Methodology Bovine enamel subsurface lesions (n=168) were selected according to their surface hardness (SH) and randomly divided into seven groups (n=24/group): Placebo (without F/TMP), 4,500 ppm F (4500F), 4500F + 2.5% TMPnano (2.5% Nano), 4500F + 5% TMPnano (5% Nano), 4500F + 5% TMPmicro (5% Micro), 9,000 ppm F (9000F), and 12,300 ppm F (Acid gel). The gels were applied in a thin layer for one minute. Half of the blocks were subjected to pH cycling for six days, whereas the remaining specimens were used for loosely- (calcium fluoride; CaF2) and firmly-bound (fluorapatite; FA) fluoride analysis. The percentage of surface hardness recovery (%SHR), area of subsurface lesion (ΔKHN), CaF2, FA, calcium (Ca), and phosphorus (P) on/in enamel were determined. Data (log10-transformed) were subjected to ANOVA and the Student-Newman-Keuls’ test (p<0.05). Results We observed a dose-response relation between F concentrations in the gels without TMP for %SHR and ΔKHN. The 2.5% Nano and 5% Micro reached similar %SHR when compared with 9000F and Acid gels. For ΔKHN, Placebo and 5% Nano gels had the highest values, and 5% Micro, 2.5% Nano, 9000F, and Acid gels, the lowest. All groups had similar retained CaF2 values, except for Placebo and Acid gel. We verified observed an increase in Ca concentrations in nano-sized TMP groups. Regarding P, TMP groups showed similar formation and retention to 9000F and Acid. Conclusion Adding 2.5% nano-sized or 5% micrometric TMP to low-fluoride gels lead to enhanced in vitro remineralization of artificial caries lesions. |
| first_indexed | 2024-03-13T02:19:25Z |
| format | Article |
| id | doaj.art-d0526f279150487b879466badf9b7ceb |
| institution | Directory Open Access Journal |
| issn | 1678-7765 |
| language | English |
| last_indexed | 2024-03-13T02:19:25Z |
| publishDate | 2023-06-01 |
| publisher | University of São Paulo |
| record_format | Article |
| series | Journal of Applied Oral Science |
| spelling | doaj.art-d0526f279150487b879466badf9b7ceb2023-06-30T10:56:44ZengUniversity of São PauloJournal of Applied Oral Science1678-77652023-06-013110.1590/1678-7757-2023-0115Effect of fluoride gels with nano-sized sodium trimetaphosphate on the in vitro remineralization of caries lesionsMariana Emi NAGATAAlberto Carlos Botazzo DELBEMhttps://orcid.org/0000-0002-8159-4853Liliana Carolina BÁEZ-QUINTEROMarcelle DANELONhttps://orcid.org/0000-0003-2091-649XCaio SAMPAIODouglas Roberto MONTEIROAnnette WIEGANDhttps://orcid.org/0000-0001-5640-8775Juliano Pelim PESSANhttps://orcid.org/0000-0002-1550-3933Abstract Objective To evaluate the effects of fluoride (F) gels supplemented with micrometric or nano-sized sodium trimetaphosphate (TMPmicro and TMPnano, respectively) on the in vitro remineralization of caries-like lesions. Methodology Bovine enamel subsurface lesions (n=168) were selected according to their surface hardness (SH) and randomly divided into seven groups (n=24/group): Placebo (without F/TMP), 4,500 ppm F (4500F), 4500F + 2.5% TMPnano (2.5% Nano), 4500F + 5% TMPnano (5% Nano), 4500F + 5% TMPmicro (5% Micro), 9,000 ppm F (9000F), and 12,300 ppm F (Acid gel). The gels were applied in a thin layer for one minute. Half of the blocks were subjected to pH cycling for six days, whereas the remaining specimens were used for loosely- (calcium fluoride; CaF2) and firmly-bound (fluorapatite; FA) fluoride analysis. The percentage of surface hardness recovery (%SHR), area of subsurface lesion (ΔKHN), CaF2, FA, calcium (Ca), and phosphorus (P) on/in enamel were determined. Data (log10-transformed) were subjected to ANOVA and the Student-Newman-Keuls’ test (p<0.05). Results We observed a dose-response relation between F concentrations in the gels without TMP for %SHR and ΔKHN. The 2.5% Nano and 5% Micro reached similar %SHR when compared with 9000F and Acid gels. For ΔKHN, Placebo and 5% Nano gels had the highest values, and 5% Micro, 2.5% Nano, 9000F, and Acid gels, the lowest. All groups had similar retained CaF2 values, except for Placebo and Acid gel. We verified observed an increase in Ca concentrations in nano-sized TMP groups. Regarding P, TMP groups showed similar formation and retention to 9000F and Acid. Conclusion Adding 2.5% nano-sized or 5% micrometric TMP to low-fluoride gels lead to enhanced in vitro remineralization of artificial caries lesions.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-77572023000100430&tlng=enFluoridesPhosphatesDental EnamelNanoparticlesNanotechnology |
| spellingShingle | Mariana Emi NAGATA Alberto Carlos Botazzo DELBEM Liliana Carolina BÁEZ-QUINTERO Marcelle DANELON Caio SAMPAIO Douglas Roberto MONTEIRO Annette WIEGAND Juliano Pelim PESSAN Effect of fluoride gels with nano-sized sodium trimetaphosphate on the in vitro remineralization of caries lesions Journal of Applied Oral Science Fluorides Phosphates Dental Enamel Nanoparticles Nanotechnology |
| title | Effect of fluoride gels with nano-sized sodium trimetaphosphate on the in vitro remineralization of caries lesions |
| title_full | Effect of fluoride gels with nano-sized sodium trimetaphosphate on the in vitro remineralization of caries lesions |
| title_fullStr | Effect of fluoride gels with nano-sized sodium trimetaphosphate on the in vitro remineralization of caries lesions |
| title_full_unstemmed | Effect of fluoride gels with nano-sized sodium trimetaphosphate on the in vitro remineralization of caries lesions |
| title_short | Effect of fluoride gels with nano-sized sodium trimetaphosphate on the in vitro remineralization of caries lesions |
| title_sort | effect of fluoride gels with nano sized sodium trimetaphosphate on the in vitro remineralization of caries lesions |
| topic | Fluorides Phosphates Dental Enamel Nanoparticles Nanotechnology |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-77572023000100430&tlng=en |
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