Lrit1, a Retinal Transmembrane Protein, Regulates Selective Synapse Formation in Cone Photoreceptor Cells and Visual Acuity
Summary: In the vertebrate retina, cone photoreceptors play crucial roles in photopic vision by transmitting light-evoked signals to ON- and/or OFF-bipolar cells. However, the mechanisms underlying selective synapse formation in the cone photoreceptor pathway remain poorly understood. Here, we found...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2018-03-01
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| Series: | Cell Reports |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2211124718303218 |
| _version_ | 1811238474773168128 |
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| author | Akiko Ueno Yoshihiro Omori Yuko Sugita Satoshi Watanabe Taro Chaya Takashi Kozuka Tetsuo Kon Satoyo Yoshida Kenji Matsushita Ryusuke Kuwahara Naoko Kajimura Yasushi Okada Takahisa Furukawa |
| author_facet | Akiko Ueno Yoshihiro Omori Yuko Sugita Satoshi Watanabe Taro Chaya Takashi Kozuka Tetsuo Kon Satoyo Yoshida Kenji Matsushita Ryusuke Kuwahara Naoko Kajimura Yasushi Okada Takahisa Furukawa |
| author_sort | Akiko Ueno |
| collection | DOAJ |
| description | Summary: In the vertebrate retina, cone photoreceptors play crucial roles in photopic vision by transmitting light-evoked signals to ON- and/or OFF-bipolar cells. However, the mechanisms underlying selective synapse formation in the cone photoreceptor pathway remain poorly understood. Here, we found that Lrit1, a leucine-rich transmembrane protein, localizes to the photoreceptor synaptic terminal and regulates the synaptic connection between cone photoreceptors and cone ON-bipolar cells. Lrit1-deficient retinas exhibit an aberrant morphology of cone photoreceptor pedicles, as well as an impairment of signal transmission from cone photoreceptors to cone ON-bipolar cells. Furthermore, we demonstrated that Lrit1 interacts with Frmpd2, a photoreceptor scaffold protein, and with mGluR6, an ON-bipolar cell-specific glutamate receptor. Additionally, Lrit1-null mice showed visual acuity impairments in their optokinetic responses. These results suggest that the Frmpd2-Lrit1-mGluR6 axis regulates selective synapse formation in cone photoreceptors and is essential for normal visual function. : Ueno et al. finds that Lrit1 plays an important role in regulating the synaptic connection between cone photoreceptors and cone ON-bipolar cells. The Frmpd2-Lrit1-mGluR6 axis is crucial for selective synapse formation in cone photoreceptors and for development of normal visual function. Keywords: retina, circuit, synapse formation, cone photoreceptor cell, ON-bipolar cell, visual acuity |
| first_indexed | 2024-04-12T12:42:07Z |
| format | Article |
| id | doaj.art-d0527a428b204301a31f9ffba765da04 |
| institution | Directory Open Access Journal |
| issn | 2211-1247 |
| language | English |
| last_indexed | 2024-04-12T12:42:07Z |
| publishDate | 2018-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Cell Reports |
| spelling | doaj.art-d0527a428b204301a31f9ffba765da042022-12-22T03:32:45ZengElsevierCell Reports2211-12472018-03-01221335483561Lrit1, a Retinal Transmembrane Protein, Regulates Selective Synapse Formation in Cone Photoreceptor Cells and Visual AcuityAkiko Ueno0Yoshihiro Omori1Yuko Sugita2Satoshi Watanabe3Taro Chaya4Takashi Kozuka5Tetsuo Kon6Satoyo Yoshida7Kenji Matsushita8Ryusuke Kuwahara9Naoko Kajimura10Yasushi Okada11Takahisa Furukawa12Laboratory for Molecular and Developmental Biology, Institute for Protein Research, Osaka University, Suita, Osaka 565-0871, JapanLaboratory for Molecular and Developmental Biology, Institute for Protein Research, Osaka University, Suita, Osaka 565-0871, JapanLaboratory for Molecular and Developmental Biology, Institute for Protein Research, Osaka University, Suita, Osaka 565-0871, JapanLaboratory for Molecular and Developmental Biology, Institute for Protein Research, Osaka University, Suita, Osaka 565-0871, JapanLaboratory for Molecular and Developmental Biology, Institute for Protein Research, Osaka University, Suita, Osaka 565-0871, JapanLaboratory for Molecular and Developmental Biology, Institute for Protein Research, Osaka University, Suita, Osaka 565-0871, JapanLaboratory for Molecular and Developmental Biology, Institute for Protein Research, Osaka University, Suita, Osaka 565-0871, JapanLaboratory for Molecular and Developmental Biology, Institute for Protein Research, Osaka University, Suita, Osaka 565-0871, JapanDepartment of Ophthalmology, Osaka University Graduate School of Medicine, Suita, Osaka 565-0871, JapanResearch Center for Ultrahigh Voltage Electron Microscopy, Osaka University, Ibaraki, Osaka 567-0047, JapanResearch Center for Ultrahigh Voltage Electron Microscopy, Osaka University, Ibaraki, Osaka 567-0047, JapanLaboratory for Cell Polarity Regulation, Quantitative Biology Center, Riken, Suita, Osaka 565-0874, JapanLaboratory for Molecular and Developmental Biology, Institute for Protein Research, Osaka University, Suita, Osaka 565-0871, Japan; Corresponding authorSummary: In the vertebrate retina, cone photoreceptors play crucial roles in photopic vision by transmitting light-evoked signals to ON- and/or OFF-bipolar cells. However, the mechanisms underlying selective synapse formation in the cone photoreceptor pathway remain poorly understood. Here, we found that Lrit1, a leucine-rich transmembrane protein, localizes to the photoreceptor synaptic terminal and regulates the synaptic connection between cone photoreceptors and cone ON-bipolar cells. Lrit1-deficient retinas exhibit an aberrant morphology of cone photoreceptor pedicles, as well as an impairment of signal transmission from cone photoreceptors to cone ON-bipolar cells. Furthermore, we demonstrated that Lrit1 interacts with Frmpd2, a photoreceptor scaffold protein, and with mGluR6, an ON-bipolar cell-specific glutamate receptor. Additionally, Lrit1-null mice showed visual acuity impairments in their optokinetic responses. These results suggest that the Frmpd2-Lrit1-mGluR6 axis regulates selective synapse formation in cone photoreceptors and is essential for normal visual function. : Ueno et al. finds that Lrit1 plays an important role in regulating the synaptic connection between cone photoreceptors and cone ON-bipolar cells. The Frmpd2-Lrit1-mGluR6 axis is crucial for selective synapse formation in cone photoreceptors and for development of normal visual function. Keywords: retina, circuit, synapse formation, cone photoreceptor cell, ON-bipolar cell, visual acuityhttp://www.sciencedirect.com/science/article/pii/S2211124718303218 |
| spellingShingle | Akiko Ueno Yoshihiro Omori Yuko Sugita Satoshi Watanabe Taro Chaya Takashi Kozuka Tetsuo Kon Satoyo Yoshida Kenji Matsushita Ryusuke Kuwahara Naoko Kajimura Yasushi Okada Takahisa Furukawa Lrit1, a Retinal Transmembrane Protein, Regulates Selective Synapse Formation in Cone Photoreceptor Cells and Visual Acuity Cell Reports |
| title | Lrit1, a Retinal Transmembrane Protein, Regulates Selective Synapse Formation in Cone Photoreceptor Cells and Visual Acuity |
| title_full | Lrit1, a Retinal Transmembrane Protein, Regulates Selective Synapse Formation in Cone Photoreceptor Cells and Visual Acuity |
| title_fullStr | Lrit1, a Retinal Transmembrane Protein, Regulates Selective Synapse Formation in Cone Photoreceptor Cells and Visual Acuity |
| title_full_unstemmed | Lrit1, a Retinal Transmembrane Protein, Regulates Selective Synapse Formation in Cone Photoreceptor Cells and Visual Acuity |
| title_short | Lrit1, a Retinal Transmembrane Protein, Regulates Selective Synapse Formation in Cone Photoreceptor Cells and Visual Acuity |
| title_sort | lrit1 a retinal transmembrane protein regulates selective synapse formation in cone photoreceptor cells and visual acuity |
| url | http://www.sciencedirect.com/science/article/pii/S2211124718303218 |
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