MAPK9 is Correlated with a Poor Prognosis and Tumor Progression in Glioma
Background: Glioma has a high incidence in young and middle-aged adults and a poor prognosis. Because of late diagnosis and uncontrollable recurrence of the primary tumor after failure of existing treatments, glioma patients tend to have a poor prognosis. Recent advances in research have revealed th...
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| Format: | Article |
| Language: | English |
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IMR Press
2023-03-01
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| Series: | Frontiers in Bioscience-Landmark |
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| Online Access: | https://www.imrpress.com/journal/FBL/28/3/10.31083/j.fbl2803063 |
| _version_ | 1797853548476628992 |
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| author | Xinyu Yang Qingge Jia Xuantong Liu Weidong Wu Yuwei Han Zheng Zou Mingyang Li Di Fan Junyang Song Ligang Chen |
| author_facet | Xinyu Yang Qingge Jia Xuantong Liu Weidong Wu Yuwei Han Zheng Zou Mingyang Li Di Fan Junyang Song Ligang Chen |
| author_sort | Xinyu Yang |
| collection | DOAJ |
| description | Background: Glioma has a high incidence in young and middle-aged adults and a poor prognosis. Because of late diagnosis and uncontrollable recurrence of the primary tumor after failure of existing treatments, glioma patients tend to have a poor prognosis. Recent advances in research have revealed that gliomas exhibit unique genetic features. Mitogen-activated protein kinase 9 (MAPK9) is significantly upregulated in mesenchymal glioma spheres and may be a new target for glioma diagnosis. This study aimed to investigate the potential diagnostic significance and predictive value of MAPK9 in glioma. Methods: Paraffin-embedded tumor tissues and paracancerous tissues were collected from 150 glioma patients seen at the General Hospital of Northern Theater Command. Immunohistochemistry and western blot assays were used to detect the expression levels of MAPK9. Prognosis and survival analyses were performed using SPSS 26 software for univariate/multivariate analysis and log-rank analysis. Cellular models were used to assess the effect of MAPK9 overexpression and knockdown in vitro. Results: MAPK9 expression was higher in glioma tissues than in paraneoplastic tissues. Prognostic and survival analyses revealed that the MAPK9 expression level is an independent prognostic factor in glioma patients. In addition, overexpression of MAPK9 significantly promoted the proliferation and migration of primary glioma cells, possibly via the Wnt/β-catenin-regulated EMT pathway. Conclusions: MAPK9 is an independent prognostic factor in glioma and is involved in tumor progression. |
| first_indexed | 2024-04-09T19:51:18Z |
| format | Article |
| id | doaj.art-d053726db9a74d6e8d8bdfbeaf9945f1 |
| institution | Directory Open Access Journal |
| issn | 2768-6701 |
| language | English |
| last_indexed | 2024-04-09T19:51:18Z |
| publishDate | 2023-03-01 |
| publisher | IMR Press |
| record_format | Article |
| series | Frontiers in Bioscience-Landmark |
| spelling | doaj.art-d053726db9a74d6e8d8bdfbeaf9945f12023-04-03T05:52:10ZengIMR PressFrontiers in Bioscience-Landmark2768-67012023-03-012836310.31083/j.fbl2803063S2768-6701(22)00751-1MAPK9 is Correlated with a Poor Prognosis and Tumor Progression in GliomaXinyu Yang0Qingge Jia1Xuantong Liu2Weidong Wu3Yuwei Han4Zheng Zou5Mingyang Li6Di Fan7Junyang Song8Ligang Chen9Department of Neurosurgery, General Hospital of Northern Theater Command, 110016 Shenyang, Liaoning, ChinaDepartment of Reproductive Endocrinology, Xi'an International Medical Center Hospital, Northwest University, 710127 Xi’an, Shaanxi, ChinaDepartment of Pathology, General Hospital of Northern Theater Command, 110016 Shenyang, Liaoning, ChinaDepartment of Neurosurgery, General Hospital of Northern Theater Command, 110016 Shenyang, Liaoning, ChinaDepartment of Neurosurgery, General Hospital of Northern Theater Command, 110016 Shenyang, Liaoning, ChinaDepartment of Neurosurgery, General Hospital of Northern Theater Command, 110016 Shenyang, Liaoning, ChinaState Key Laboratory of Cancer Biology, Department of Pathology, Xijing Hospital and School of Basic Medicine, Fourth Military Medical University, 710068 Xi’an, Shaanxi, ChinaDepartment of Neurosurgery, General Hospital of Northern Theater Command, 110016 Shenyang, Liaoning, ChinaState Key Laboratory of Cancer Biology, Department of Pathology, Xijing Hospital and School of Basic Medicine, Fourth Military Medical University, 710068 Xi’an, Shaanxi, ChinaDepartment of Neurosurgery, General Hospital of Northern Theater Command, 110016 Shenyang, Liaoning, ChinaBackground: Glioma has a high incidence in young and middle-aged adults and a poor prognosis. Because of late diagnosis and uncontrollable recurrence of the primary tumor after failure of existing treatments, glioma patients tend to have a poor prognosis. Recent advances in research have revealed that gliomas exhibit unique genetic features. Mitogen-activated protein kinase 9 (MAPK9) is significantly upregulated in mesenchymal glioma spheres and may be a new target for glioma diagnosis. This study aimed to investigate the potential diagnostic significance and predictive value of MAPK9 in glioma. Methods: Paraffin-embedded tumor tissues and paracancerous tissues were collected from 150 glioma patients seen at the General Hospital of Northern Theater Command. Immunohistochemistry and western blot assays were used to detect the expression levels of MAPK9. Prognosis and survival analyses were performed using SPSS 26 software for univariate/multivariate analysis and log-rank analysis. Cellular models were used to assess the effect of MAPK9 overexpression and knockdown in vitro. Results: MAPK9 expression was higher in glioma tissues than in paraneoplastic tissues. Prognostic and survival analyses revealed that the MAPK9 expression level is an independent prognostic factor in glioma patients. In addition, overexpression of MAPK9 significantly promoted the proliferation and migration of primary glioma cells, possibly via the Wnt/β-catenin-regulated EMT pathway. Conclusions: MAPK9 is an independent prognostic factor in glioma and is involved in tumor progression.https://www.imrpress.com/journal/FBL/28/3/10.31083/j.fbl2803063mapk9gliomaclinicopathological variablesprognosis |
| spellingShingle | Xinyu Yang Qingge Jia Xuantong Liu Weidong Wu Yuwei Han Zheng Zou Mingyang Li Di Fan Junyang Song Ligang Chen MAPK9 is Correlated with a Poor Prognosis and Tumor Progression in Glioma Frontiers in Bioscience-Landmark mapk9 glioma clinicopathological variables prognosis |
| title | MAPK9 is Correlated with a Poor Prognosis and Tumor Progression in Glioma |
| title_full | MAPK9 is Correlated with a Poor Prognosis and Tumor Progression in Glioma |
| title_fullStr | MAPK9 is Correlated with a Poor Prognosis and Tumor Progression in Glioma |
| title_full_unstemmed | MAPK9 is Correlated with a Poor Prognosis and Tumor Progression in Glioma |
| title_short | MAPK9 is Correlated with a Poor Prognosis and Tumor Progression in Glioma |
| title_sort | mapk9 is correlated with a poor prognosis and tumor progression in glioma |
| topic | mapk9 glioma clinicopathological variables prognosis |
| url | https://www.imrpress.com/journal/FBL/28/3/10.31083/j.fbl2803063 |
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