Comparative analysis of genomes of tick-borne encephalitis virus strains isolated from mosquitoes and ticks
The tick-borne encephalitis virus (TBEV) strain Lazo MP36 was isolated from the pool of mosquitoes Aedes vexans collected in Lazo region of Khabarovsk territory in August 2014. Phylogenetic analysis of the strain Lazo MP36 complete genome (GenBank accession number KT001073) revealed its corresponden...
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| Format: | Article |
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Central Research Institute for Epidemiology
2017-02-01
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| Series: | Вопросы вирусологии |
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| Online Access: | https://virusjour.crie.ru/jour/article/viewFile/126/46 |
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| author | N. M. Pukhovskaya O. V. Morozova N. B. Belozerova S. V. Bakhmetyeva N. P. Vysochina N. I. Zdanovskaya L. I. Ivanov |
| author_facet | N. M. Pukhovskaya O. V. Morozova N. B. Belozerova S. V. Bakhmetyeva N. P. Vysochina N. I. Zdanovskaya L. I. Ivanov |
| author_sort | N. M. Pukhovskaya |
| collection | DOAJ |
| description | The tick-borne encephalitis virus (TBEV) strain Lazo MP36 was isolated from the pool of mosquitoes Aedes vexans collected in Lazo region of Khabarovsk territory in August 2014. Phylogenetic analysis of the strain Lazo MP36 complete genome (GenBank accession number KT001073) revealed its correspondence to the TBEV Far Eastern subtype and differences from the following strains: 1) from ticks Ixodes persulcatus P. Schulze, 1930 [vaccine strain 205 (JX498939) and strains Khekhtzir 1230 (KF880805), Chichagovka (KP844724), Birobidzhan 1354 (KF880805) isolated in 2012-2013]; 2) from mosquitoes [strain Malyshevo (KJ744034) isolated in 1978 from Aedes vexans nipponii in Khabarovsk territory; strain Sakhalin 6-11 isolated from the pool of mosquitoes in 2011 (KF826916)]; 3) from human brain [vaccine strain Sofjin (JN229223), Glubinnoe/2004(DQ862460). Kavalerovo (DQ862460), Svetlogorie (DQ862460)]. The fusion peptide necessary for flavivirus entry to cells of the three TBEV strains isolated from mosquitoes (Lazo MP36, Malyshevo and Sakhalin 6-11) has the canonical structure 98-DRGWGNHCGLFGKGSI-113 for the tick-borne flaviviruses. Amino acid transition H104G typical for the mosquito-borne flaviviruses was not found. Structures of 5’- and 3’-untranslated (UTR) regions of the TBEV strains from mosquitoes were 85-98% homologous to the TBEV strains of all subtypes without recombination with mosquito-borne flaviviruses found in the Far East of Russia. Secondary structures of 5’- and 3'-UTR as well as cyclization sequences (CS) of types a and B are highly homologous for all TBEV isolates independently of the biological hosts and vectors. similarity of the genomes of the TBEV isolates from mosquitoes, ticks and patients as well as pathogenicity of the isolates for new-borne laboratory mice and tissue cultures might suggest a possible role of mosquitoes in the TBEV circulation in natural foci as an accidental or additional virus carrier. |
| first_indexed | 2024-03-13T00:08:08Z |
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| issn | 0507-4088 2411-2097 |
| language | English |
| last_indexed | 2024-03-13T00:08:08Z |
| publishDate | 2017-02-01 |
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| spelling | doaj.art-d054508b778c40dd95a867b4561437fa2023-07-12T20:01:55ZengCentral Research Institute for EpidemiologyВопросы вирусологии0507-40882411-20972017-02-01621303510.18821/0507-4088-2017-62-1-30-35107Comparative analysis of genomes of tick-borne encephalitis virus strains isolated from mosquitoes and ticksN. M. Pukhovskaya0O. V. Morozova1N. B. Belozerova2S. V. Bakhmetyeva3N. P. Vysochina4N. I. Zdanovskaya5L. I. Ivanov6Khabarovsk Antiplague StationFederal Research Centre for Epidemiology and Microbiology named after the honorary academician N.F. GamaleyaKhabarovsk Antiplague StationKhabarovsk Antiplague StationKhabarovsk Antiplague StationKhabarovsk Antiplague StationKhabarovsk Antiplague StationThe tick-borne encephalitis virus (TBEV) strain Lazo MP36 was isolated from the pool of mosquitoes Aedes vexans collected in Lazo region of Khabarovsk territory in August 2014. Phylogenetic analysis of the strain Lazo MP36 complete genome (GenBank accession number KT001073) revealed its correspondence to the TBEV Far Eastern subtype and differences from the following strains: 1) from ticks Ixodes persulcatus P. Schulze, 1930 [vaccine strain 205 (JX498939) and strains Khekhtzir 1230 (KF880805), Chichagovka (KP844724), Birobidzhan 1354 (KF880805) isolated in 2012-2013]; 2) from mosquitoes [strain Malyshevo (KJ744034) isolated in 1978 from Aedes vexans nipponii in Khabarovsk territory; strain Sakhalin 6-11 isolated from the pool of mosquitoes in 2011 (KF826916)]; 3) from human brain [vaccine strain Sofjin (JN229223), Glubinnoe/2004(DQ862460). Kavalerovo (DQ862460), Svetlogorie (DQ862460)]. The fusion peptide necessary for flavivirus entry to cells of the three TBEV strains isolated from mosquitoes (Lazo MP36, Malyshevo and Sakhalin 6-11) has the canonical structure 98-DRGWGNHCGLFGKGSI-113 for the tick-borne flaviviruses. Amino acid transition H104G typical for the mosquito-borne flaviviruses was not found. Structures of 5’- and 3’-untranslated (UTR) regions of the TBEV strains from mosquitoes were 85-98% homologous to the TBEV strains of all subtypes without recombination with mosquito-borne flaviviruses found in the Far East of Russia. Secondary structures of 5’- and 3'-UTR as well as cyclization sequences (CS) of types a and B are highly homologous for all TBEV isolates independently of the biological hosts and vectors. similarity of the genomes of the TBEV isolates from mosquitoes, ticks and patients as well as pathogenicity of the isolates for new-borne laboratory mice and tissue cultures might suggest a possible role of mosquitoes in the TBEV circulation in natural foci as an accidental or additional virus carrier.https://virusjour.crie.ru/jour/article/viewFile/126/46tick-borne and mosquito-borne flavivirusesphylogenetic analysis of complete coding regions of genomesfusion peptideuntranslated regionscyclization sequences |
| spellingShingle | N. M. Pukhovskaya O. V. Morozova N. B. Belozerova S. V. Bakhmetyeva N. P. Vysochina N. I. Zdanovskaya L. I. Ivanov Comparative analysis of genomes of tick-borne encephalitis virus strains isolated from mosquitoes and ticks Вопросы вирусологии tick-borne and mosquito-borne flaviviruses phylogenetic analysis of complete coding regions of genomes fusion peptide untranslated regions cyclization sequences |
| title | Comparative analysis of genomes of tick-borne encephalitis virus strains isolated from mosquitoes and ticks |
| title_full | Comparative analysis of genomes of tick-borne encephalitis virus strains isolated from mosquitoes and ticks |
| title_fullStr | Comparative analysis of genomes of tick-borne encephalitis virus strains isolated from mosquitoes and ticks |
| title_full_unstemmed | Comparative analysis of genomes of tick-borne encephalitis virus strains isolated from mosquitoes and ticks |
| title_short | Comparative analysis of genomes of tick-borne encephalitis virus strains isolated from mosquitoes and ticks |
| title_sort | comparative analysis of genomes of tick borne encephalitis virus strains isolated from mosquitoes and ticks |
| topic | tick-borne and mosquito-borne flaviviruses phylogenetic analysis of complete coding regions of genomes fusion peptide untranslated regions cyclization sequences |
| url | https://virusjour.crie.ru/jour/article/viewFile/126/46 |
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