Glial and neuronal Semaphorin signaling instruct the development of a functional myotopic map for Drosophila walking
Motoneurons developmentally acquire appropriate cellular architectures that ensure connections with postsynaptic muscles and presynaptic neurons. In Drosophila, leg motoneurons are organized as a myotopic map, where their dendritic domains represent the muscle field. Here, we investigate mechanisms...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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eLife Sciences Publications Ltd
2016-02-01
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| Series: | eLife |
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| Online Access: | https://elifesciences.org/articles/11572 |
| _version_ | 1811227875449241600 |
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| author | Durafshan Sakeena Syed Swetha B.M. Gowda O Venkateswara Reddy Heinrich Reichert K VijayRaghavan |
| author_facet | Durafshan Sakeena Syed Swetha B.M. Gowda O Venkateswara Reddy Heinrich Reichert K VijayRaghavan |
| author_sort | Durafshan Sakeena Syed |
| collection | DOAJ |
| description | Motoneurons developmentally acquire appropriate cellular architectures that ensure connections with postsynaptic muscles and presynaptic neurons. In Drosophila, leg motoneurons are organized as a myotopic map, where their dendritic domains represent the muscle field. Here, we investigate mechanisms underlying development of aspects of this myotopic map, required for walking. A behavioral screen identified roles for Semaphorins (Sema) and Plexins (Plex) in walking behavior. Deciphering this phenotype, we show that PlexA/Sema1a mediates motoneuron axon branching in ways that differ in the proximal femur and distal tibia, based on motoneuronal birth order. Importantly, we show a novel role for glia in positioning dendrites of specific motoneurons; PlexB/Sema2a is required for dendritic positioning of late-born motoneurons but not early-born motoneurons. These findings indicate that communication within motoneurons and between glia and motoneurons, mediated by the combined action of different Plexin/Semaphorin signaling systems, are required for the formation of a functional myotopic map. |
| first_indexed | 2024-04-12T09:48:05Z |
| format | Article |
| id | doaj.art-d0551c3f455c4c2d8db633597fe8803d |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-12T09:48:05Z |
| publishDate | 2016-02-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-d0551c3f455c4c2d8db633597fe8803d2022-12-22T03:37:53ZengeLife Sciences Publications LtdeLife2050-084X2016-02-01510.7554/eLife.11572Glial and neuronal Semaphorin signaling instruct the development of a functional myotopic map for Drosophila walkingDurafshan Sakeena Syed0Swetha B.M. Gowda1O Venkateswara Reddy2Heinrich Reichert3K VijayRaghavan4https://orcid.org/0000-0002-4705-5629National Centre for Biological Sciences, Tata Institute of Fundamental Research, Bangalore, IndiaNational Centre for Biological Sciences, Tata Institute of Fundamental Research, Bangalore, India; Manipal University, Manipal, IndiaNational Centre for Biological Sciences, Tata Institute of Fundamental Research, Bangalore, IndiaBiozentrum, University of Basel, Basel, SwitzerlandNational Centre for Biological Sciences, Tata Institute of Fundamental Research, Bangalore, IndiaMotoneurons developmentally acquire appropriate cellular architectures that ensure connections with postsynaptic muscles and presynaptic neurons. In Drosophila, leg motoneurons are organized as a myotopic map, where their dendritic domains represent the muscle field. Here, we investigate mechanisms underlying development of aspects of this myotopic map, required for walking. A behavioral screen identified roles for Semaphorins (Sema) and Plexins (Plex) in walking behavior. Deciphering this phenotype, we show that PlexA/Sema1a mediates motoneuron axon branching in ways that differ in the proximal femur and distal tibia, based on motoneuronal birth order. Importantly, we show a novel role for glia in positioning dendrites of specific motoneurons; PlexB/Sema2a is required for dendritic positioning of late-born motoneurons but not early-born motoneurons. These findings indicate that communication within motoneurons and between glia and motoneurons, mediated by the combined action of different Plexin/Semaphorin signaling systems, are required for the formation of a functional myotopic map.https://elifesciences.org/articles/11572semaphorinmotor neuronsmyotopic mapneuron targetingglia |
| spellingShingle | Durafshan Sakeena Syed Swetha B.M. Gowda O Venkateswara Reddy Heinrich Reichert K VijayRaghavan Glial and neuronal Semaphorin signaling instruct the development of a functional myotopic map for Drosophila walking eLife semaphorin motor neurons myotopic map neuron targeting glia |
| title | Glial and neuronal Semaphorin signaling instruct the development of a functional myotopic map for Drosophila walking |
| title_full | Glial and neuronal Semaphorin signaling instruct the development of a functional myotopic map for Drosophila walking |
| title_fullStr | Glial and neuronal Semaphorin signaling instruct the development of a functional myotopic map for Drosophila walking |
| title_full_unstemmed | Glial and neuronal Semaphorin signaling instruct the development of a functional myotopic map for Drosophila walking |
| title_short | Glial and neuronal Semaphorin signaling instruct the development of a functional myotopic map for Drosophila walking |
| title_sort | glial and neuronal semaphorin signaling instruct the development of a functional myotopic map for drosophila walking |
| topic | semaphorin motor neurons myotopic map neuron targeting glia |
| url | https://elifesciences.org/articles/11572 |
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