Genome-Wide Identification and Characterization of Cysteine-Rich Receptor-Like Protein Kinase Genes in Tomato and Their Expression Profile in Response to Heat Stress
During plant growth, development and stress adaption, receptor-like protein kinases (RLKs) are essential components in perceiving and integrating extracellular stimuli and transmitting the signals to activate the downstream signaling pathways. Cysteine-rich receptor-like protein kinases (CRKs) are a...
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MDPI AG
2021-06-01
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| Online Access: | https://www.mdpi.com/1424-2818/13/6/258 |
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| author | Yahui Liu Zhengxiang Feng Weimin Zhu Junzhong Liu Yingying Zhang |
| author_facet | Yahui Liu Zhengxiang Feng Weimin Zhu Junzhong Liu Yingying Zhang |
| author_sort | Yahui Liu |
| collection | DOAJ |
| description | During plant growth, development and stress adaption, receptor-like protein kinases (RLKs) are essential components in perceiving and integrating extracellular stimuli and transmitting the signals to activate the downstream signaling pathways. Cysteine-rich receptor-like protein kinases (CRKs) are a large subfamily of RLKs and their roles in modulating plant disease resistance are well elucidated. However, the roles of CRKs in plant abiotic stress responses, especially heat stress, are largely unknown. In this study, 35 <i>SlCRK</i> genes were identified in tomato (<i>Solanum lycopersicum</i>) based on the multiple sequence alignment and phylogenetic relationships. <i>SlCRK</i> genes are tandemly distributed on seven chromosomes and have similar exon–intron organization and common conserved motifs. Various phytohormone responsive, stress responsive cis-regulatory elements and heat shock elements are predicted in the promoter regions of <i>SlCRK</i> genes. Transcriptome analysis of tomato fruits under heat stress revealed that most <i>SlCRK</i> genes were downregulated upon heat treatment. GO enrichment analyses of genes that were co-expressed with <i>SlCRK</i> members have identified various stress responses related and proteasomal protein catabolic process related genes, which may be involved in heat stress signaling. Overall, our results provide valuable information for further research on the roles of <i>SlCRK</i>s in response to abiotic stress, especially heat stress. |
| first_indexed | 2024-03-10T10:32:38Z |
| format | Article |
| id | doaj.art-d058ead089d4403ba539b9e6b0baea34 |
| institution | Directory Open Access Journal |
| issn | 1424-2818 |
| language | English |
| last_indexed | 2024-03-10T10:32:38Z |
| publishDate | 2021-06-01 |
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| spelling | doaj.art-d058ead089d4403ba539b9e6b0baea342023-11-21T23:34:09ZengMDPI AGDiversity1424-28182021-06-0113625810.3390/d13060258Genome-Wide Identification and Characterization of Cysteine-Rich Receptor-Like Protein Kinase Genes in Tomato and Their Expression Profile in Response to Heat StressYahui Liu0Zhengxiang Feng1Weimin Zhu2Junzhong Liu3Yingying Zhang4Shanghai Key Laboratory of Protected Horticulture Technology, The Protected Horticulture Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201403, ChinaState Key Laboratory of Conservation and Utilization of Bio-Resources in Yunnan and Center for Life Sciences, School of Life Sciences, Yunnan University, Kunming 650091, ChinaShanghai Key Laboratory of Protected Horticulture Technology, The Protected Horticulture Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201403, ChinaState Key Laboratory of Conservation and Utilization of Bio-Resources in Yunnan and Center for Life Sciences, School of Life Sciences, Yunnan University, Kunming 650091, ChinaShanghai Key Laboratory of Protected Horticulture Technology, The Protected Horticulture Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201403, ChinaDuring plant growth, development and stress adaption, receptor-like protein kinases (RLKs) are essential components in perceiving and integrating extracellular stimuli and transmitting the signals to activate the downstream signaling pathways. Cysteine-rich receptor-like protein kinases (CRKs) are a large subfamily of RLKs and their roles in modulating plant disease resistance are well elucidated. However, the roles of CRKs in plant abiotic stress responses, especially heat stress, are largely unknown. In this study, 35 <i>SlCRK</i> genes were identified in tomato (<i>Solanum lycopersicum</i>) based on the multiple sequence alignment and phylogenetic relationships. <i>SlCRK</i> genes are tandemly distributed on seven chromosomes and have similar exon–intron organization and common conserved motifs. Various phytohormone responsive, stress responsive cis-regulatory elements and heat shock elements are predicted in the promoter regions of <i>SlCRK</i> genes. Transcriptome analysis of tomato fruits under heat stress revealed that most <i>SlCRK</i> genes were downregulated upon heat treatment. GO enrichment analyses of genes that were co-expressed with <i>SlCRK</i> members have identified various stress responses related and proteasomal protein catabolic process related genes, which may be involved in heat stress signaling. Overall, our results provide valuable information for further research on the roles of <i>SlCRK</i>s in response to abiotic stress, especially heat stress.https://www.mdpi.com/1424-2818/13/6/258CRKheat stress resistance<i>Solanum lycopersicum</i>genome-wide profilingexpression regulation |
| spellingShingle | Yahui Liu Zhengxiang Feng Weimin Zhu Junzhong Liu Yingying Zhang Genome-Wide Identification and Characterization of Cysteine-Rich Receptor-Like Protein Kinase Genes in Tomato and Their Expression Profile in Response to Heat Stress Diversity CRK heat stress resistance <i>Solanum lycopersicum</i> genome-wide profiling expression regulation |
| title | Genome-Wide Identification and Characterization of Cysteine-Rich Receptor-Like Protein Kinase Genes in Tomato and Their Expression Profile in Response to Heat Stress |
| title_full | Genome-Wide Identification and Characterization of Cysteine-Rich Receptor-Like Protein Kinase Genes in Tomato and Their Expression Profile in Response to Heat Stress |
| title_fullStr | Genome-Wide Identification and Characterization of Cysteine-Rich Receptor-Like Protein Kinase Genes in Tomato and Their Expression Profile in Response to Heat Stress |
| title_full_unstemmed | Genome-Wide Identification and Characterization of Cysteine-Rich Receptor-Like Protein Kinase Genes in Tomato and Their Expression Profile in Response to Heat Stress |
| title_short | Genome-Wide Identification and Characterization of Cysteine-Rich Receptor-Like Protein Kinase Genes in Tomato and Their Expression Profile in Response to Heat Stress |
| title_sort | genome wide identification and characterization of cysteine rich receptor like protein kinase genes in tomato and their expression profile in response to heat stress |
| topic | CRK heat stress resistance <i>Solanum lycopersicum</i> genome-wide profiling expression regulation |
| url | https://www.mdpi.com/1424-2818/13/6/258 |
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