Toward the Next Frontiers of Vibrational Bioimaging
Chemical imaging based on vibrational contrasts can extract molecular information entangled in complex biological systems. To this end, nonlinear Raman scattering microscopy, mid-infrared photothermal (MIP) microscopy, and atomic force microscopy (AFM)-based force-detected photothermal microscopies...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
American Chemical Society
2023-03-01
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| Series: | Chemical & Biomedical Imaging |
| Online Access: | https://doi.org/10.1021/cbmi.3c00004 |
| _version_ | 1797739855814328320 |
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| author | Haomin Wang Dongkwan Lee Lu Wei |
| author_facet | Haomin Wang Dongkwan Lee Lu Wei |
| author_sort | Haomin Wang |
| collection | DOAJ |
| description | Chemical imaging based on vibrational contrasts can extract molecular information entangled in complex biological systems. To this end, nonlinear Raman scattering microscopy, mid-infrared photothermal (MIP) microscopy, and atomic force microscopy (AFM)-based force-detected photothermal microscopies are emerging with better chemical sensitivity, molecular specificity, and spatial resolution than conventional vibrational methods. Their utilization in bioimaging applications has provided biological knowledge in unprecedented detail. This Perspective outlines key methodological developments, bioimaging applications, and recent technical innovations of the three techniques. Representative biological demonstrations are also highlighted to exemplify the unique advantages of obtaining vibrational contrasts. With years of effort, these three methods compose an expanding vibrational bioimaging toolbox to tackle specific bioimaging needs, benefiting many biological investigations with rich information in both label-free and labeling manners. Each technique will be discussed and compared in the outlook, leading to possible future directions to accommodate growing needs in vibrational bioimaging. |
| first_indexed | 2024-03-12T14:04:07Z |
| format | Article |
| id | doaj.art-d058ffdbaefb493b9eb4d6d5ba2f3e46 |
| institution | Directory Open Access Journal |
| issn | 2832-3637 |
| language | English |
| last_indexed | 2024-03-12T14:04:07Z |
| publishDate | 2023-03-01 |
| publisher | American Chemical Society |
| record_format | Article |
| series | Chemical & Biomedical Imaging |
| spelling | doaj.art-d058ffdbaefb493b9eb4d6d5ba2f3e462023-08-21T17:52:15ZengAmerican Chemical SocietyChemical & Biomedical Imaging2832-36372023-03-011131710.1021/cbmi.3c00004Toward the Next Frontiers of Vibrational BioimagingHaomin Wang0Dongkwan Lee1Lu Wei2Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, California, United StatesDivision of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, California, United StatesDivision of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, California, United StatesChemical imaging based on vibrational contrasts can extract molecular information entangled in complex biological systems. To this end, nonlinear Raman scattering microscopy, mid-infrared photothermal (MIP) microscopy, and atomic force microscopy (AFM)-based force-detected photothermal microscopies are emerging with better chemical sensitivity, molecular specificity, and spatial resolution than conventional vibrational methods. Their utilization in bioimaging applications has provided biological knowledge in unprecedented detail. This Perspective outlines key methodological developments, bioimaging applications, and recent technical innovations of the three techniques. Representative biological demonstrations are also highlighted to exemplify the unique advantages of obtaining vibrational contrasts. With years of effort, these three methods compose an expanding vibrational bioimaging toolbox to tackle specific bioimaging needs, benefiting many biological investigations with rich information in both label-free and labeling manners. Each technique will be discussed and compared in the outlook, leading to possible future directions to accommodate growing needs in vibrational bioimaging.https://doi.org/10.1021/cbmi.3c00004 |
| spellingShingle | Haomin Wang Dongkwan Lee Lu Wei Toward the Next Frontiers of Vibrational Bioimaging Chemical & Biomedical Imaging |
| title | Toward the Next Frontiers of Vibrational Bioimaging |
| title_full | Toward the Next Frontiers of Vibrational Bioimaging |
| title_fullStr | Toward the Next Frontiers of Vibrational Bioimaging |
| title_full_unstemmed | Toward the Next Frontiers of Vibrational Bioimaging |
| title_short | Toward the Next Frontiers of Vibrational Bioimaging |
| title_sort | toward the next frontiers of vibrational bioimaging |
| url | https://doi.org/10.1021/cbmi.3c00004 |
| work_keys_str_mv | AT haominwang towardthenextfrontiersofvibrationalbioimaging AT dongkwanlee towardthenextfrontiersofvibrationalbioimaging AT luwei towardthenextfrontiersofvibrationalbioimaging |