Benefits of Polyphenol-Based Synbiotics in Crustacean Diet
Here, the olive leaf extract (OLE) rich in polyphenols was employed as a prebiotic agent, together with <i>Lactobacillus reuteri</i> and <i>Bacillus clausii</i>, to develop synbiotics. The prebiotic effect of olive leaf extract on the probiotic strains was tested at concentra...
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| Format: | Article |
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MDPI AG
2023-05-01
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| Series: | Fishes |
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| Online Access: | https://www.mdpi.com/2410-3888/8/5/255 |
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| author | Daniela Sateriale Serena Facchiano Katrin Kaldre Giuseppina Forgione Giuseppa Anna De Cristofaro Caterina Pagliarulo Marina Paolucci |
| author_facet | Daniela Sateriale Serena Facchiano Katrin Kaldre Giuseppina Forgione Giuseppa Anna De Cristofaro Caterina Pagliarulo Marina Paolucci |
| author_sort | Daniela Sateriale |
| collection | DOAJ |
| description | Here, the olive leaf extract (OLE) rich in polyphenols was employed as a prebiotic agent, together with <i>Lactobacillus reuteri</i> and <i>Bacillus clausii</i>, to develop synbiotics. The prebiotic effect of olive leaf extract on the probiotic strains was tested at concentrations of 0, 50, 100, 400, and 1000 μg mL<sup>−1</sup>, and also 20 and 40 mg mL<sup>−1</sup>. Olive leaf extract at 40 mg mL<sup>−1</sup> showed the best prebiotic activity on <i>L. reuteri</i> and <i>B. clausii</i>. A basal diet and two experimental synbiotic-containing diets were prepared. The synbiotic diets were manufactured by adding to the basal diet 5 × 10<sup>6</sup> CFU g<sup>−1</sup> <i>L. reuteri</i> + 5 × 10<sup>6</sup> CFU g<sup>−1</sup> <i>B. clausii</i> + 0.25 mg g<sup>−1</sup> OLE and 1 × 10<sup>7</sup> CFU g<sup>−1</sup> <i>L. reuteri</i> + 1 × 10<sup>7</sup> CFU g<sup>−1</sup> <i>B. clausii</i> + 0.25 mg g<sup>−1</sup> OLE. The diets were administered to the freshwater crayfish <i>Astacus astacus</i> (1.35 ± 0.04 g) in an 84-day feeding trial. The diet containing 5 × 10<sup>6</sup> CFU g<sup>−1</sup> <i>L. reuteri</i> + 5 × 10<sup>6</sup> CFU g<sup>−1</sup> <i>B. clausii</i> + 0.25 mg g<sup>−1</sup> OLE significantly improved (<i>p</i> < 0.05) final weight, specific growth rate, body condition, and survival rate. A significant growth of <i>Enterobacteriaceae</i>, which include strains with proven beneficial activities for intestinal health and general animal welfare, significantly increased in crayfish fed with synbiotics. The obtained results could be suitable for functional feed development in crayfish farming. |
| first_indexed | 2024-03-11T03:45:08Z |
| format | Article |
| id | doaj.art-d0592344002a43108828b9bd4dc89090 |
| institution | Directory Open Access Journal |
| issn | 2410-3888 |
| language | English |
| last_indexed | 2024-03-11T03:45:08Z |
| publishDate | 2023-05-01 |
| publisher | MDPI AG |
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| spelling | doaj.art-d0592344002a43108828b9bd4dc890902023-11-18T01:19:53ZengMDPI AGFishes2410-38882023-05-018525510.3390/fishes8050255Benefits of Polyphenol-Based Synbiotics in Crustacean DietDaniela Sateriale0Serena Facchiano1Katrin Kaldre2Giuseppina Forgione3Giuseppa Anna De Cristofaro4Caterina Pagliarulo5Marina Paolucci6Department of Sciences and Technologies, University of Sannio, Via De Sanctis, snc, 82100 Benevento, ItalyDepartment of Sciences and Technologies, University of Sannio, Via De Sanctis, snc, 82100 Benevento, ItalyInstitute of Veterinary Medicine and Animal Sciences, Estonian University of Life Sciences, Kreutzwaldi 46A, 51006 Tartu, EstoniaDepartment of Sciences and Technologies, University of Sannio, Via De Sanctis, snc, 82100 Benevento, ItalyDepartment of Sciences and Technologies, University of Sannio, Via De Sanctis, snc, 82100 Benevento, ItalyDepartment of Sciences and Technologies, University of Sannio, Via De Sanctis, snc, 82100 Benevento, ItalyDepartment of Sciences and Technologies, University of Sannio, Via De Sanctis, snc, 82100 Benevento, ItalyHere, the olive leaf extract (OLE) rich in polyphenols was employed as a prebiotic agent, together with <i>Lactobacillus reuteri</i> and <i>Bacillus clausii</i>, to develop synbiotics. The prebiotic effect of olive leaf extract on the probiotic strains was tested at concentrations of 0, 50, 100, 400, and 1000 μg mL<sup>−1</sup>, and also 20 and 40 mg mL<sup>−1</sup>. Olive leaf extract at 40 mg mL<sup>−1</sup> showed the best prebiotic activity on <i>L. reuteri</i> and <i>B. clausii</i>. A basal diet and two experimental synbiotic-containing diets were prepared. The synbiotic diets were manufactured by adding to the basal diet 5 × 10<sup>6</sup> CFU g<sup>−1</sup> <i>L. reuteri</i> + 5 × 10<sup>6</sup> CFU g<sup>−1</sup> <i>B. clausii</i> + 0.25 mg g<sup>−1</sup> OLE and 1 × 10<sup>7</sup> CFU g<sup>−1</sup> <i>L. reuteri</i> + 1 × 10<sup>7</sup> CFU g<sup>−1</sup> <i>B. clausii</i> + 0.25 mg g<sup>−1</sup> OLE. The diets were administered to the freshwater crayfish <i>Astacus astacus</i> (1.35 ± 0.04 g) in an 84-day feeding trial. The diet containing 5 × 10<sup>6</sup> CFU g<sup>−1</sup> <i>L. reuteri</i> + 5 × 10<sup>6</sup> CFU g<sup>−1</sup> <i>B. clausii</i> + 0.25 mg g<sup>−1</sup> OLE significantly improved (<i>p</i> < 0.05) final weight, specific growth rate, body condition, and survival rate. A significant growth of <i>Enterobacteriaceae</i>, which include strains with proven beneficial activities for intestinal health and general animal welfare, significantly increased in crayfish fed with synbiotics. The obtained results could be suitable for functional feed development in crayfish farming.https://www.mdpi.com/2410-3888/8/5/255polyphenolsprebioticsprobioticssynbioticscrustaceans<i>Astacus astacus</i> |
| spellingShingle | Daniela Sateriale Serena Facchiano Katrin Kaldre Giuseppina Forgione Giuseppa Anna De Cristofaro Caterina Pagliarulo Marina Paolucci Benefits of Polyphenol-Based Synbiotics in Crustacean Diet Fishes polyphenols prebiotics probiotics synbiotics crustaceans <i>Astacus astacus</i> |
| title | Benefits of Polyphenol-Based Synbiotics in Crustacean Diet |
| title_full | Benefits of Polyphenol-Based Synbiotics in Crustacean Diet |
| title_fullStr | Benefits of Polyphenol-Based Synbiotics in Crustacean Diet |
| title_full_unstemmed | Benefits of Polyphenol-Based Synbiotics in Crustacean Diet |
| title_short | Benefits of Polyphenol-Based Synbiotics in Crustacean Diet |
| title_sort | benefits of polyphenol based synbiotics in crustacean diet |
| topic | polyphenols prebiotics probiotics synbiotics crustaceans <i>Astacus astacus</i> |
| url | https://www.mdpi.com/2410-3888/8/5/255 |
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