Simple Microcontact Printing Technique to Obtain Cell Patterns by Lithography Using Grayscale, Photopolymer Flexographic Mold, and PDMS
Microcontact printing using PDMS embossing tools and its variations have aroused the interest of a wide spectrum of research fields, hence the feasibility of defining micro and nanoscale patterns. In this work, we have proposed and demonstrated a novel lithography method based on grayscale patterns...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-10-01
|
| Series: | Biomimetics |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2313-7673/7/4/155 |
| _version_ | 1797461315063644160 |
|---|---|
| author | Rocio Gimenez Camilo Pérez-Sosa Natalia Bourguignon Santiago Miriuka Shekhar Bhansali Carlos R. Arroyo Alexis Debut Betiana Lerner Maximiliano S. Pérez |
| author_facet | Rocio Gimenez Camilo Pérez-Sosa Natalia Bourguignon Santiago Miriuka Shekhar Bhansali Carlos R. Arroyo Alexis Debut Betiana Lerner Maximiliano S. Pérez |
| author_sort | Rocio Gimenez |
| collection | DOAJ |
| description | Microcontact printing using PDMS embossing tools and its variations have aroused the interest of a wide spectrum of research fields, hence the feasibility of defining micro and nanoscale patterns. In this work, we have proposed and demonstrated a novel lithography method based on grayscale patterns printed in a flexographic photopolymer mold and transferred to epoxy resin and a single PDMS stamp to obtain different microprint pattern structures. The geometry of the patterns can be modified by adjusting the layout and grayscale of the stamp patterns. The functionality of this contact printing methodology was validated by generating human induced pluripotent stem cells (hiPSC) patterns. These specific micropatterns can be very useful for achieving complex differentiation in cell lines such as hiPSC. Microfabrication through the new technique provides a promising alternative to conventional lithography for constructing complex aligned surfaces; these structures could be used as components of biological patterns or microfluidic devices. |
| first_indexed | 2024-03-09T17:17:39Z |
| format | Article |
| id | doaj.art-d05b7f227b09449381c986aeb2af1371 |
| institution | Directory Open Access Journal |
| issn | 2313-7673 |
| language | English |
| last_indexed | 2024-03-09T17:17:39Z |
| publishDate | 2022-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomimetics |
| spelling | doaj.art-d05b7f227b09449381c986aeb2af13712023-11-24T13:30:31ZengMDPI AGBiomimetics2313-76732022-10-017415510.3390/biomimetics7040155Simple Microcontact Printing Technique to Obtain Cell Patterns by Lithography Using Grayscale, Photopolymer Flexographic Mold, and PDMSRocio Gimenez0Camilo Pérez-Sosa1Natalia Bourguignon2Santiago Miriuka3Shekhar Bhansali4Carlos R. Arroyo5Alexis Debut6Betiana Lerner7Maximiliano S. Pérez8IREN Center, National Technological University, Buenos Aires 1706, ArgentinaIREN Center, National Technological University, Buenos Aires 1706, ArgentinaIREN Center, National Technological University, Buenos Aires 1706, ArgentinaLIAN-CONICET-FLENI, Ruta 9 Km 52, 5, Belén de Escobar 1625, ArgentinaDepartment of Electrical and Computer Engineering, Florida International University, Miami, FL 33174, USACentro de Nanociencia y Nanotecnología, Universidad de las Fuerzas Armadas ESPE, Sangolqui P.O. Box 171-5-231B, EcuadorCentro de Nanociencia y Nanotecnología, Universidad de las Fuerzas Armadas ESPE, Sangolqui P.O. Box 171-5-231B, EcuadorIREN Center, National Technological University, Buenos Aires 1706, ArgentinaIREN Center, National Technological University, Buenos Aires 1706, ArgentinaMicrocontact printing using PDMS embossing tools and its variations have aroused the interest of a wide spectrum of research fields, hence the feasibility of defining micro and nanoscale patterns. In this work, we have proposed and demonstrated a novel lithography method based on grayscale patterns printed in a flexographic photopolymer mold and transferred to epoxy resin and a single PDMS stamp to obtain different microprint pattern structures. The geometry of the patterns can be modified by adjusting the layout and grayscale of the stamp patterns. The functionality of this contact printing methodology was validated by generating human induced pluripotent stem cells (hiPSC) patterns. These specific micropatterns can be very useful for achieving complex differentiation in cell lines such as hiPSC. Microfabrication through the new technique provides a promising alternative to conventional lithography for constructing complex aligned surfaces; these structures could be used as components of biological patterns or microfluidic devices.https://www.mdpi.com/2313-7673/7/4/155grayscalePDMS stampflexographic photopolymer moldcontact printing3D patterns |
| spellingShingle | Rocio Gimenez Camilo Pérez-Sosa Natalia Bourguignon Santiago Miriuka Shekhar Bhansali Carlos R. Arroyo Alexis Debut Betiana Lerner Maximiliano S. Pérez Simple Microcontact Printing Technique to Obtain Cell Patterns by Lithography Using Grayscale, Photopolymer Flexographic Mold, and PDMS Biomimetics grayscale PDMS stamp flexographic photopolymer mold contact printing 3D patterns |
| title | Simple Microcontact Printing Technique to Obtain Cell Patterns by Lithography Using Grayscale, Photopolymer Flexographic Mold, and PDMS |
| title_full | Simple Microcontact Printing Technique to Obtain Cell Patterns by Lithography Using Grayscale, Photopolymer Flexographic Mold, and PDMS |
| title_fullStr | Simple Microcontact Printing Technique to Obtain Cell Patterns by Lithography Using Grayscale, Photopolymer Flexographic Mold, and PDMS |
| title_full_unstemmed | Simple Microcontact Printing Technique to Obtain Cell Patterns by Lithography Using Grayscale, Photopolymer Flexographic Mold, and PDMS |
| title_short | Simple Microcontact Printing Technique to Obtain Cell Patterns by Lithography Using Grayscale, Photopolymer Flexographic Mold, and PDMS |
| title_sort | simple microcontact printing technique to obtain cell patterns by lithography using grayscale photopolymer flexographic mold and pdms |
| topic | grayscale PDMS stamp flexographic photopolymer mold contact printing 3D patterns |
| url | https://www.mdpi.com/2313-7673/7/4/155 |
| work_keys_str_mv | AT rociogimenez simplemicrocontactprintingtechniquetoobtaincellpatternsbylithographyusinggrayscalephotopolymerflexographicmoldandpdms AT camiloperezsosa simplemicrocontactprintingtechniquetoobtaincellpatternsbylithographyusinggrayscalephotopolymerflexographicmoldandpdms AT nataliabourguignon simplemicrocontactprintingtechniquetoobtaincellpatternsbylithographyusinggrayscalephotopolymerflexographicmoldandpdms AT santiagomiriuka simplemicrocontactprintingtechniquetoobtaincellpatternsbylithographyusinggrayscalephotopolymerflexographicmoldandpdms AT shekharbhansali simplemicrocontactprintingtechniquetoobtaincellpatternsbylithographyusinggrayscalephotopolymerflexographicmoldandpdms AT carlosrarroyo simplemicrocontactprintingtechniquetoobtaincellpatternsbylithographyusinggrayscalephotopolymerflexographicmoldandpdms AT alexisdebut simplemicrocontactprintingtechniquetoobtaincellpatternsbylithographyusinggrayscalephotopolymerflexographicmoldandpdms AT betianalerner simplemicrocontactprintingtechniquetoobtaincellpatternsbylithographyusinggrayscalephotopolymerflexographicmoldandpdms AT maximilianosperez simplemicrocontactprintingtechniquetoobtaincellpatternsbylithographyusinggrayscalephotopolymerflexographicmoldandpdms |