Chemical Composition of Oil Fraction Kaffir Lime (Citrus hystrix DC) as Antibacterial Activity of E.coli
The purpose of this research is to investigate the composition of oil fraction kaffir lime which is consists as antibacterial activity of E. coli. This research was applied a branch kaffir lime to produce oil using fractional distillation (PiloDist 104-VTU) number the stages 120 and reflux ratio 20/...
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| Format: | Article |
| Language: | English |
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University of Brawijaya
2018-01-01
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| Series: | Journal of Pure and Applied Chemistry Research |
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| Online Access: | http://jpacr.ub.ac.id/index.php/jpacr/article/view/352/pdf |
| _version_ | 1818407690181804032 |
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| author | Rahmatika Ayu Habsari Warsito Noorhamdani |
| author_facet | Rahmatika Ayu Habsari Warsito Noorhamdani |
| author_sort | Rahmatika Ayu Habsari |
| collection | DOAJ |
| description | The purpose of this research is to investigate the composition of oil fraction kaffir lime which is consists as antibacterial activity of E. coli. This research was applied a branch kaffir lime to produce oil using fractional distillation (PiloDist 104-VTU) number the stages 120 and reflux ratio 20/10 with 5 mbar pressure. Oil kaffir lime composition was analyzed using GC-MS (type Shimadzu QP 2010S) by helium as a carrier gas with flow rate of 3mL/min. Antibacterial activity assay was employed agar well diffusion which conducted at three concentrations (500, 300, and 100 µL/mL). The result of oil fraction kaffir lime was afforded five fraction oil based on boiling point interval, such as A fraction oil (63.00 – 70.010 oC), B fraction (71.30 – 70.800 oC), C fraction (74.50 – 74.200 oC), D fraction (74.20 – 74.000 oC) and E fraction (72.90 – 91.100 oC). All fractions contained oxygenated monoterpene (MO), except A oil fraction which comprises hydrocarbon monoterpene composition (MH) with a yield of 12.1%. The main components of a fraction which MO compound they are citronella, linalool and isopulegol, while in MH compound they are sabine, β-pinene, β-micrene and limonene. The result of antibacterial activity assay obtained at the highest concentration (500 µL/mL). Antibacterial activity assay also depends on the fraction composition with higher composition of MO. The highest MO component of oil fraction was found on C oil fraction which has MO component such as citronella 74.94%; linalool 20.13%; and isopulegol 3.08%. |
| first_indexed | 2024-12-14T09:31:50Z |
| format | Article |
| id | doaj.art-d05dd8445c8048a9933c7e77f0ce7692 |
| institution | Directory Open Access Journal |
| issn | 2302-4690 2302-4690 |
| language | English |
| last_indexed | 2024-12-14T09:31:50Z |
| publishDate | 2018-01-01 |
| publisher | University of Brawijaya |
| record_format | Article |
| series | Journal of Pure and Applied Chemistry Research |
| spelling | doaj.art-d05dd8445c8048a9933c7e77f0ce76922022-12-21T23:08:02ZengUniversity of BrawijayaJournal of Pure and Applied Chemistry Research2302-46902302-46902018-01-0171333910.21776/ub.jpacr.2018.007.01.352Chemical Composition of Oil Fraction Kaffir Lime (Citrus hystrix DC) as Antibacterial Activity of E.coliRahmatika Ayu Habsari 0Warsito1Noorhamdani2Department of Chemistry, Faculty of Mathematics and Natural Science, Universitas BrawijayaDepartment of Chemistry, Faculty of Mathematics and Natural Science, Universitas BrawijayaDepartment of Medical Faculty, Universitas BrawijayaThe purpose of this research is to investigate the composition of oil fraction kaffir lime which is consists as antibacterial activity of E. coli. This research was applied a branch kaffir lime to produce oil using fractional distillation (PiloDist 104-VTU) number the stages 120 and reflux ratio 20/10 with 5 mbar pressure. Oil kaffir lime composition was analyzed using GC-MS (type Shimadzu QP 2010S) by helium as a carrier gas with flow rate of 3mL/min. Antibacterial activity assay was employed agar well diffusion which conducted at three concentrations (500, 300, and 100 µL/mL). The result of oil fraction kaffir lime was afforded five fraction oil based on boiling point interval, such as A fraction oil (63.00 – 70.010 oC), B fraction (71.30 – 70.800 oC), C fraction (74.50 – 74.200 oC), D fraction (74.20 – 74.000 oC) and E fraction (72.90 – 91.100 oC). All fractions contained oxygenated monoterpene (MO), except A oil fraction which comprises hydrocarbon monoterpene composition (MH) with a yield of 12.1%. The main components of a fraction which MO compound they are citronella, linalool and isopulegol, while in MH compound they are sabine, β-pinene, β-micrene and limonene. The result of antibacterial activity assay obtained at the highest concentration (500 µL/mL). Antibacterial activity assay also depends on the fraction composition with higher composition of MO. The highest MO component of oil fraction was found on C oil fraction which has MO component such as citronella 74.94%; linalool 20.13%; and isopulegol 3.08%.http://jpacr.ub.ac.id/index.php/jpacr/article/view/352/pdfKaffir lime oil fractionantibacterial activityE. colihydrocarbon monoterpenoidoxygenated monoterpenoid |
| spellingShingle | Rahmatika Ayu Habsari Warsito Noorhamdani Chemical Composition of Oil Fraction Kaffir Lime (Citrus hystrix DC) as Antibacterial Activity of E.coli Journal of Pure and Applied Chemistry Research Kaffir lime oil fraction antibacterial activity E. coli hydrocarbon monoterpenoid oxygenated monoterpenoid |
| title | Chemical Composition of Oil Fraction Kaffir Lime (Citrus hystrix DC) as Antibacterial Activity of E.coli |
| title_full | Chemical Composition of Oil Fraction Kaffir Lime (Citrus hystrix DC) as Antibacterial Activity of E.coli |
| title_fullStr | Chemical Composition of Oil Fraction Kaffir Lime (Citrus hystrix DC) as Antibacterial Activity of E.coli |
| title_full_unstemmed | Chemical Composition of Oil Fraction Kaffir Lime (Citrus hystrix DC) as Antibacterial Activity of E.coli |
| title_short | Chemical Composition of Oil Fraction Kaffir Lime (Citrus hystrix DC) as Antibacterial Activity of E.coli |
| title_sort | chemical composition of oil fraction kaffir lime citrus hystrix dc as antibacterial activity of e coli |
| topic | Kaffir lime oil fraction antibacterial activity E. coli hydrocarbon monoterpenoid oxygenated monoterpenoid |
| url | http://jpacr.ub.ac.id/index.php/jpacr/article/view/352/pdf |
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