The genomic characterisation and comparison of Bacillus cereus strains isolated from indoor air
Abstract Background Bacillus cereus is ubiquitous in nature, found in environments such as soil, plants, air, and part of the insect and human gut microbiome. The ability to produce endospores and biofilms contribute to their pathogenicity, classified in two types of food poisoning: diarrheal and em...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2021-01-01
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| Series: | Gut Pathogens |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13099-021-00399-4 |
| _version_ | 1828889894759432192 |
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| author | Balakrishnan N. V. Premkrishnan Cassie E. Heinle Akira Uchida Rikky W. Purbojati Kavita K. Kushwaha Alexander Putra Puramadathil Sasi Santhi Benjamin W. Y. Khoo Anthony Wong Vineeth Kodengil Vettath Daniela I. Drautz-Moses Ana Carolina M. Junqueira Stephan C. Schuster |
| author_facet | Balakrishnan N. V. Premkrishnan Cassie E. Heinle Akira Uchida Rikky W. Purbojati Kavita K. Kushwaha Alexander Putra Puramadathil Sasi Santhi Benjamin W. Y. Khoo Anthony Wong Vineeth Kodengil Vettath Daniela I. Drautz-Moses Ana Carolina M. Junqueira Stephan C. Schuster |
| author_sort | Balakrishnan N. V. Premkrishnan |
| collection | DOAJ |
| description | Abstract Background Bacillus cereus is ubiquitous in nature, found in environments such as soil, plants, air, and part of the insect and human gut microbiome. The ability to produce endospores and biofilms contribute to their pathogenicity, classified in two types of food poisoning: diarrheal and emetic syndromes. Here we report gap-free, whole-genome sequences of two B. cereus strains isolated from air samples and analyse their emetic and diarrheal potential. Results Genome assemblies of the B. cereus strains consist of one chromosome and seven plasmids each. The genome size of strain SGAir0260 is 6.30-Mb with 6590 predicted coding sequences (CDS) and strain SGAir0263 is 6.47-Mb with 6811 predicted CDS. Macrosynteny analysis showed 99% collinearity between the strains isolated from air and 90.2% with the reference genome. Comparative genomics with 57 complete B. cereus genomes suggests these strains from air are closely associated with strains isolated from foodborne illnesses outbreaks. Due to virulence potential of B. cereus and its reported involvement in nosocomial infections, antibiotic resistance analyses were performed and confirmed resistance to ampicillin and fosfomycin, with susceptibility to ciprofloxacin, tetracycline and vancomycin in both strains. Conclusion Phylogenetic analysis combined with detection of haemolytic (hblA, hblC, and hblD) and non-haemolytic (nheA, nheB, and nheC) enterotoxin genes in both air-isolated strains point to the diarrheic potential of the air isolates, though not emetic. Characterization of these airborne strains and investigation of their potential disease-causing genes could facilitate identification of environmental sources of contamination leading to foodborne illnesses and nosocomial infections transported by air. |
| first_indexed | 2024-12-13T12:49:13Z |
| format | Article |
| id | doaj.art-d05ecb6459f94b2da9c5527978a1e12a |
| institution | Directory Open Access Journal |
| issn | 1757-4749 |
| language | English |
| last_indexed | 2024-12-13T12:49:13Z |
| publishDate | 2021-01-01 |
| publisher | BMC |
| record_format | Article |
| series | Gut Pathogens |
| spelling | doaj.art-d05ecb6459f94b2da9c5527978a1e12a2022-12-21T23:45:24ZengBMCGut Pathogens1757-47492021-01-0113111010.1186/s13099-021-00399-4The genomic characterisation and comparison of Bacillus cereus strains isolated from indoor airBalakrishnan N. V. Premkrishnan0Cassie E. Heinle1Akira Uchida2Rikky W. Purbojati3Kavita K. Kushwaha4Alexander Putra5Puramadathil Sasi Santhi6Benjamin W. Y. Khoo7Anthony Wong8Vineeth Kodengil Vettath9Daniela I. Drautz-Moses10Ana Carolina M. Junqueira11Stephan C. Schuster12Singapore Centre for Environmental Life Sciences Engineering, Nanyang Technological UniversitySingapore Centre for Environmental Life Sciences Engineering, Nanyang Technological UniversitySingapore Centre for Environmental Life Sciences Engineering, Nanyang Technological UniversitySingapore Centre for Environmental Life Sciences Engineering, Nanyang Technological UniversitySingapore Centre for Environmental Life Sciences Engineering, Nanyang Technological UniversitySingapore Centre for Environmental Life Sciences Engineering, Nanyang Technological UniversitySingapore Centre for Environmental Life Sciences Engineering, Nanyang Technological UniversitySingapore Centre for Environmental Life Sciences Engineering, Nanyang Technological UniversitySingapore Centre for Environmental Life Sciences Engineering, Nanyang Technological UniversitySingapore Centre for Environmental Life Sciences Engineering, Nanyang Technological UniversitySingapore Centre for Environmental Life Sciences Engineering, Nanyang Technological UniversityDepartamento de Genética, Instituto de Biologia, Universidade Federal do Rio de JaneiroSingapore Centre for Environmental Life Sciences Engineering, Nanyang Technological UniversityAbstract Background Bacillus cereus is ubiquitous in nature, found in environments such as soil, plants, air, and part of the insect and human gut microbiome. The ability to produce endospores and biofilms contribute to their pathogenicity, classified in two types of food poisoning: diarrheal and emetic syndromes. Here we report gap-free, whole-genome sequences of two B. cereus strains isolated from air samples and analyse their emetic and diarrheal potential. Results Genome assemblies of the B. cereus strains consist of one chromosome and seven plasmids each. The genome size of strain SGAir0260 is 6.30-Mb with 6590 predicted coding sequences (CDS) and strain SGAir0263 is 6.47-Mb with 6811 predicted CDS. Macrosynteny analysis showed 99% collinearity between the strains isolated from air and 90.2% with the reference genome. Comparative genomics with 57 complete B. cereus genomes suggests these strains from air are closely associated with strains isolated from foodborne illnesses outbreaks. Due to virulence potential of B. cereus and its reported involvement in nosocomial infections, antibiotic resistance analyses were performed and confirmed resistance to ampicillin and fosfomycin, with susceptibility to ciprofloxacin, tetracycline and vancomycin in both strains. Conclusion Phylogenetic analysis combined with detection of haemolytic (hblA, hblC, and hblD) and non-haemolytic (nheA, nheB, and nheC) enterotoxin genes in both air-isolated strains point to the diarrheic potential of the air isolates, though not emetic. Characterization of these airborne strains and investigation of their potential disease-causing genes could facilitate identification of environmental sources of contamination leading to foodborne illnesses and nosocomial infections transported by air.https://doi.org/10.1186/s13099-021-00399-4Bacillus cereusFood poisoningAntibiotic resistanceEnterotoxins |
| spellingShingle | Balakrishnan N. V. Premkrishnan Cassie E. Heinle Akira Uchida Rikky W. Purbojati Kavita K. Kushwaha Alexander Putra Puramadathil Sasi Santhi Benjamin W. Y. Khoo Anthony Wong Vineeth Kodengil Vettath Daniela I. Drautz-Moses Ana Carolina M. Junqueira Stephan C. Schuster The genomic characterisation and comparison of Bacillus cereus strains isolated from indoor air Gut Pathogens Bacillus cereus Food poisoning Antibiotic resistance Enterotoxins |
| title | The genomic characterisation and comparison of Bacillus cereus strains isolated from indoor air |
| title_full | The genomic characterisation and comparison of Bacillus cereus strains isolated from indoor air |
| title_fullStr | The genomic characterisation and comparison of Bacillus cereus strains isolated from indoor air |
| title_full_unstemmed | The genomic characterisation and comparison of Bacillus cereus strains isolated from indoor air |
| title_short | The genomic characterisation and comparison of Bacillus cereus strains isolated from indoor air |
| title_sort | genomic characterisation and comparison of bacillus cereus strains isolated from indoor air |
| topic | Bacillus cereus Food poisoning Antibiotic resistance Enterotoxins |
| url | https://doi.org/10.1186/s13099-021-00399-4 |
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