Genome-Wide Identification of PAP1 Direct Targets in Regulating Seed Anthocyanin Biosynthesis in <i>Arabidopsis</i>

Anthocyanins are widespread water-soluble pigments in the plant kingdom. Anthocyanin accumulation is activated by the MYB-bHLH-WD40 (MBW) protein complex. In <i>Arabidopsis</i>, the R2R3-MYB transcription factor PAP1 activates anthocyanin biosynthesis. While prior research primarily focu...

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Main Authors: Yuan Guo, Dong Li, Tiantian Liu, Yuxin Li, Jiajia Liu, Mingyuan He, Xiaohui Cui, Zijin Liu, Mingxun Chen
Format: Article
Language:English
Published: MDPI AG 2023-11-01
Series:International Journal of Molecular Sciences
Subjects:
Online Access:https://www.mdpi.com/1422-0067/24/22/16049
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author Yuan Guo
Dong Li
Tiantian Liu
Yuxin Li
Jiajia Liu
Mingyuan He
Xiaohui Cui
Zijin Liu
Mingxun Chen
author_facet Yuan Guo
Dong Li
Tiantian Liu
Yuxin Li
Jiajia Liu
Mingyuan He
Xiaohui Cui
Zijin Liu
Mingxun Chen
author_sort Yuan Guo
collection DOAJ
description Anthocyanins are widespread water-soluble pigments in the plant kingdom. Anthocyanin accumulation is activated by the MYB-bHLH-WD40 (MBW) protein complex. In <i>Arabidopsis</i>, the R2R3-MYB transcription factor PAP1 activates anthocyanin biosynthesis. While prior research primarily focused on seedlings, seeds received limited attention. This study explores PAP1’s genome-wide target genes in anthocyanin biosynthesis in seeds. Our findings confirm that <i>PAP1</i> is a positive regulator of anthocyanin biosynthesis in <i>Arabidopsis</i> seeds. PAP1 significantly increased anthocyanin content in developing and mature seeds in <i>Arabidopsis</i>. Transcriptome analysis at 12 days after pollination reveals the upregulation of numerous genes involved in anthocyanin accumulation in <i>35S:PAP1</i> developing seeds. Chromatin immunoprecipitation and dual luciferase reporter assays demonstrate PAP1’s direct promotion of ten key genes and indirect upregulation of <i>TT8</i>, <i>TTG1,</i> and eight key genes during seed maturation, thus enhancing seed anthocyanin accumulation. These findings enhance our understanding of PAP1’s novel role in regulating anthocyanin accumulation in <i>Arabidopsis</i> seeds.
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spelling doaj.art-d05fa36ba20d4a3281184a20486808022023-11-24T14:45:41ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-11-0124221604910.3390/ijms242216049Genome-Wide Identification of PAP1 Direct Targets in Regulating Seed Anthocyanin Biosynthesis in <i>Arabidopsis</i>Yuan Guo0Dong Li1Tiantian Liu2Yuxin Li3Jiajia Liu4Mingyuan He5Xiaohui Cui6Zijin Liu7Mingxun Chen8Shaanxi Key Laboratory of Crop Heterosis, National Yangling Agricultural Biotechnology and Breeding Center, College of Agronomy, Northwest A&F University, Yangling 712100, ChinaThe Engineering Research Institute of Agriculture and Forestry, Ludong University, Yantai 264025, ChinaShaanxi Key Laboratory of Crop Heterosis, National Yangling Agricultural Biotechnology and Breeding Center, College of Agronomy, Northwest A&F University, Yangling 712100, ChinaShaanxi Key Laboratory of Crop Heterosis, National Yangling Agricultural Biotechnology and Breeding Center, College of Agronomy, Northwest A&F University, Yangling 712100, ChinaShaanxi Key Laboratory of Crop Heterosis, National Yangling Agricultural Biotechnology and Breeding Center, College of Agronomy, Northwest A&F University, Yangling 712100, ChinaShaanxi Key Laboratory of Crop Heterosis, National Yangling Agricultural Biotechnology and Breeding Center, College of Agronomy, Northwest A&F University, Yangling 712100, ChinaShaanxi Key Laboratory of Crop Heterosis, National Yangling Agricultural Biotechnology and Breeding Center, College of Agronomy, Northwest A&F University, Yangling 712100, ChinaShaanxi Key Laboratory of Crop Heterosis, National Yangling Agricultural Biotechnology and Breeding Center, College of Agronomy, Northwest A&F University, Yangling 712100, ChinaShaanxi Key Laboratory of Crop Heterosis, National Yangling Agricultural Biotechnology and Breeding Center, College of Agronomy, Northwest A&F University, Yangling 712100, ChinaAnthocyanins are widespread water-soluble pigments in the plant kingdom. Anthocyanin accumulation is activated by the MYB-bHLH-WD40 (MBW) protein complex. In <i>Arabidopsis</i>, the R2R3-MYB transcription factor PAP1 activates anthocyanin biosynthesis. While prior research primarily focused on seedlings, seeds received limited attention. This study explores PAP1’s genome-wide target genes in anthocyanin biosynthesis in seeds. Our findings confirm that <i>PAP1</i> is a positive regulator of anthocyanin biosynthesis in <i>Arabidopsis</i> seeds. PAP1 significantly increased anthocyanin content in developing and mature seeds in <i>Arabidopsis</i>. Transcriptome analysis at 12 days after pollination reveals the upregulation of numerous genes involved in anthocyanin accumulation in <i>35S:PAP1</i> developing seeds. Chromatin immunoprecipitation and dual luciferase reporter assays demonstrate PAP1’s direct promotion of ten key genes and indirect upregulation of <i>TT8</i>, <i>TTG1,</i> and eight key genes during seed maturation, thus enhancing seed anthocyanin accumulation. These findings enhance our understanding of PAP1’s novel role in regulating anthocyanin accumulation in <i>Arabidopsis</i> seeds.https://www.mdpi.com/1422-0067/24/22/16049PAP1MBW complexanthocyanin biosynthesisseeds<i>Arabidopsis</i>
spellingShingle Yuan Guo
Dong Li
Tiantian Liu
Yuxin Li
Jiajia Liu
Mingyuan He
Xiaohui Cui
Zijin Liu
Mingxun Chen
Genome-Wide Identification of PAP1 Direct Targets in Regulating Seed Anthocyanin Biosynthesis in <i>Arabidopsis</i>
International Journal of Molecular Sciences
PAP1
MBW complex
anthocyanin biosynthesis
seeds
<i>Arabidopsis</i>
title Genome-Wide Identification of PAP1 Direct Targets in Regulating Seed Anthocyanin Biosynthesis in <i>Arabidopsis</i>
title_full Genome-Wide Identification of PAP1 Direct Targets in Regulating Seed Anthocyanin Biosynthesis in <i>Arabidopsis</i>
title_fullStr Genome-Wide Identification of PAP1 Direct Targets in Regulating Seed Anthocyanin Biosynthesis in <i>Arabidopsis</i>
title_full_unstemmed Genome-Wide Identification of PAP1 Direct Targets in Regulating Seed Anthocyanin Biosynthesis in <i>Arabidopsis</i>
title_short Genome-Wide Identification of PAP1 Direct Targets in Regulating Seed Anthocyanin Biosynthesis in <i>Arabidopsis</i>
title_sort genome wide identification of pap1 direct targets in regulating seed anthocyanin biosynthesis in i arabidopsis i
topic PAP1
MBW complex
anthocyanin biosynthesis
seeds
<i>Arabidopsis</i>
url https://www.mdpi.com/1422-0067/24/22/16049
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