Engineering bacteriophages for enhanced host range and efficacy: insights from bacteriophage-bacteria interactions
Bacteriophages, the most abundant organisms on earth, have the potential to address the rise of multidrug-resistant bacteria resulting from the overuse of antibiotics. However, their high specificity and limited host range can hinder their effectiveness. Phage engineering, through the use of gene ed...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-05-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1172635/full |
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| author | Huang-Jie Jia Huang-Jie Jia Pan-Pan Jia Supei Yin Ling-Kang Bu Guan Yang De-Sheng Pei |
| author_facet | Huang-Jie Jia Huang-Jie Jia Pan-Pan Jia Supei Yin Ling-Kang Bu Guan Yang De-Sheng Pei |
| author_sort | Huang-Jie Jia |
| collection | DOAJ |
| description | Bacteriophages, the most abundant organisms on earth, have the potential to address the rise of multidrug-resistant bacteria resulting from the overuse of antibiotics. However, their high specificity and limited host range can hinder their effectiveness. Phage engineering, through the use of gene editing techniques, offers a means to enhance the host range of bacteria, improve phage efficacy, and facilitate efficient cell-free production of phage drugs. To engineer phages effectively, it is necessary to understand the interaction between phages and host bacteria. Understanding the interaction between the receptor recognition protein of bacteriophages and host receptors can serve as a valuable guide for modifying or replacing these proteins, thereby altering the receptor range of the bacteriophage. Research and development focused on the CRISPR-Cas bacterial immune system against bacteriophage nucleic acids can provide the necessary tools to promote recombination and counter-selection in engineered bacteriophage programs. Additionally, studying the transcription and assembly functions of bacteriophages in host bacteria can facilitate the engineered assembly of bacteriophage genomes in non-host environments. This review highlights a comprehensive summary of phage engineering methods, including in-host and out-of-host engineering, and the use of high-throughput methods to understand their role. The main aim of these techniques is to harness the intricate interactions between bacteriophages and hosts to inform and guide the engineering of bacteriophages, particularly in the context of studying and manipulating the host range of bacteriophages. By employing advanced high-throughput methods to identify specific bacteriophage receptor recognition genes, and subsequently introducing modifications or performing gene swapping through in-host recombination or out-of-host synthesis, it becomes possible to strategically alter the host range of bacteriophages. This capability holds immense significance for leveraging bacteriophages as a promising therapeutic approach against antibiotic-resistant bacteria. |
| first_indexed | 2024-03-13T08:23:08Z |
| format | Article |
| id | doaj.art-d066d09bdb484f619e3f113a6b94c188 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-03-13T08:23:08Z |
| publishDate | 2023-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-d066d09bdb484f619e3f113a6b94c1882023-05-31T05:09:22ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2023-05-011410.3389/fmicb.2023.11726351172635Engineering bacteriophages for enhanced host range and efficacy: insights from bacteriophage-bacteria interactionsHuang-Jie Jia0Huang-Jie Jia1Pan-Pan Jia2Supei Yin3Ling-Kang Bu4Guan Yang5De-Sheng Pei6College of Resources and Environment, University of Chinese Academy of Sciences, Beijing, ChinaChongqing Institute of Green and Intelligent Technology, Chinese Academy of Sciences, Chongqing, ChinaSchool of Public Health, Chongqing Medical University, Chongqing, ChinaUrinary Nephropathy Center, The Second Affiliated Hospital of Chongqing Medical University, Chongqing, ChinaCollege of Life Science, Henan Normal University, Xinxiang, ChinaChongqing Institute of Green and Intelligent Technology, Chinese Academy of Sciences, Chongqing, ChinaSchool of Public Health, Chongqing Medical University, Chongqing, ChinaBacteriophages, the most abundant organisms on earth, have the potential to address the rise of multidrug-resistant bacteria resulting from the overuse of antibiotics. However, their high specificity and limited host range can hinder their effectiveness. Phage engineering, through the use of gene editing techniques, offers a means to enhance the host range of bacteria, improve phage efficacy, and facilitate efficient cell-free production of phage drugs. To engineer phages effectively, it is necessary to understand the interaction between phages and host bacteria. Understanding the interaction between the receptor recognition protein of bacteriophages and host receptors can serve as a valuable guide for modifying or replacing these proteins, thereby altering the receptor range of the bacteriophage. Research and development focused on the CRISPR-Cas bacterial immune system against bacteriophage nucleic acids can provide the necessary tools to promote recombination and counter-selection in engineered bacteriophage programs. Additionally, studying the transcription and assembly functions of bacteriophages in host bacteria can facilitate the engineered assembly of bacteriophage genomes in non-host environments. This review highlights a comprehensive summary of phage engineering methods, including in-host and out-of-host engineering, and the use of high-throughput methods to understand their role. The main aim of these techniques is to harness the intricate interactions between bacteriophages and hosts to inform and guide the engineering of bacteriophages, particularly in the context of studying and manipulating the host range of bacteriophages. By employing advanced high-throughput methods to identify specific bacteriophage receptor recognition genes, and subsequently introducing modifications or performing gene swapping through in-host recombination or out-of-host synthesis, it becomes possible to strategically alter the host range of bacteriophages. This capability holds immense significance for leveraging bacteriophages as a promising therapeutic approach against antibiotic-resistant bacteria.https://www.frontiersin.org/articles/10.3389/fmicb.2023.1172635/fullmultidrug resistant bacteriaphage engineeringgene editingphage-host interactionhigh-throughput methods |
| spellingShingle | Huang-Jie Jia Huang-Jie Jia Pan-Pan Jia Supei Yin Ling-Kang Bu Guan Yang De-Sheng Pei Engineering bacteriophages for enhanced host range and efficacy: insights from bacteriophage-bacteria interactions Frontiers in Microbiology multidrug resistant bacteria phage engineering gene editing phage-host interaction high-throughput methods |
| title | Engineering bacteriophages for enhanced host range and efficacy: insights from bacteriophage-bacteria interactions |
| title_full | Engineering bacteriophages for enhanced host range and efficacy: insights from bacteriophage-bacteria interactions |
| title_fullStr | Engineering bacteriophages for enhanced host range and efficacy: insights from bacteriophage-bacteria interactions |
| title_full_unstemmed | Engineering bacteriophages for enhanced host range and efficacy: insights from bacteriophage-bacteria interactions |
| title_short | Engineering bacteriophages for enhanced host range and efficacy: insights from bacteriophage-bacteria interactions |
| title_sort | engineering bacteriophages for enhanced host range and efficacy insights from bacteriophage bacteria interactions |
| topic | multidrug resistant bacteria phage engineering gene editing phage-host interaction high-throughput methods |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1172635/full |
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