Splicing-Mediated Autoregulation Modulates Rpl22p Expression in Saccharomyces cerevisiae.
In Saccharomyces cerevisiae, splicing is critical for expression of ribosomal protein genes (RPGs), which are among the most highly expressed genes and are tightly regulated according to growth and environmental conditions. However, knowledge of the precise mechanisms by which RPG pre-mRNA splicing...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2016-04-01
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| Series: | PLoS Genetics |
| Online Access: | http://europepmc.org/articles/PMC4838235?pdf=render |
| _version_ | 1811296226878947328 |
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| author | Jason Gabunilas Guillaume Chanfreau |
| author_facet | Jason Gabunilas Guillaume Chanfreau |
| author_sort | Jason Gabunilas |
| collection | DOAJ |
| description | In Saccharomyces cerevisiae, splicing is critical for expression of ribosomal protein genes (RPGs), which are among the most highly expressed genes and are tightly regulated according to growth and environmental conditions. However, knowledge of the precise mechanisms by which RPG pre-mRNA splicing is regulated on a gene-by-gene basis is lacking. Here we show that Rpl22p has an extraribosomal role in the inhibition of splicing of the RPL22B pre-mRNA transcript. A stem loop secondary structure within the intron is necessary for pre-mRNA binding by Rpl22p in vivo and splicing inhibition in vivo and in vitro and can rescue splicing inhibition in vitro when added in trans to splicing reactions. Splicing inhibition by Rpl22p may be partly attributed to the reduction of co-transcriptional U1 snRNP recruitment to the pre-mRNA at the RPL22B locus. We further demonstrate that the inhibition of RPL22B pre-mRNA splicing contributes to the down-regulation of mature transcript during specific stress conditions, and provide evidence hinting at a regulatory role for this mechanism in conditions of suppressed ribosome biogenesis. These results demonstrate an autoregulatory mechanism that fine-tunes the expression of the Rpl22 protein and by extension Rpl22p paralog composition according to the cellular demands for ribosome biogenesis. |
| first_indexed | 2024-04-13T05:45:10Z |
| format | Article |
| id | doaj.art-d066d5f080bd4df48203f49687277a74 |
| institution | Directory Open Access Journal |
| issn | 1553-7390 1553-7404 |
| language | English |
| last_indexed | 2024-04-13T05:45:10Z |
| publishDate | 2016-04-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Genetics |
| spelling | doaj.art-d066d5f080bd4df48203f49687277a742022-12-22T02:59:58ZengPublic Library of Science (PLoS)PLoS Genetics1553-73901553-74042016-04-01124e100599910.1371/journal.pgen.1005999Splicing-Mediated Autoregulation Modulates Rpl22p Expression in Saccharomyces cerevisiae.Jason GabunilasGuillaume ChanfreauIn Saccharomyces cerevisiae, splicing is critical for expression of ribosomal protein genes (RPGs), which are among the most highly expressed genes and are tightly regulated according to growth and environmental conditions. However, knowledge of the precise mechanisms by which RPG pre-mRNA splicing is regulated on a gene-by-gene basis is lacking. Here we show that Rpl22p has an extraribosomal role in the inhibition of splicing of the RPL22B pre-mRNA transcript. A stem loop secondary structure within the intron is necessary for pre-mRNA binding by Rpl22p in vivo and splicing inhibition in vivo and in vitro and can rescue splicing inhibition in vitro when added in trans to splicing reactions. Splicing inhibition by Rpl22p may be partly attributed to the reduction of co-transcriptional U1 snRNP recruitment to the pre-mRNA at the RPL22B locus. We further demonstrate that the inhibition of RPL22B pre-mRNA splicing contributes to the down-regulation of mature transcript during specific stress conditions, and provide evidence hinting at a regulatory role for this mechanism in conditions of suppressed ribosome biogenesis. These results demonstrate an autoregulatory mechanism that fine-tunes the expression of the Rpl22 protein and by extension Rpl22p paralog composition according to the cellular demands for ribosome biogenesis.http://europepmc.org/articles/PMC4838235?pdf=render |
| spellingShingle | Jason Gabunilas Guillaume Chanfreau Splicing-Mediated Autoregulation Modulates Rpl22p Expression in Saccharomyces cerevisiae. PLoS Genetics |
| title | Splicing-Mediated Autoregulation Modulates Rpl22p Expression in Saccharomyces cerevisiae. |
| title_full | Splicing-Mediated Autoregulation Modulates Rpl22p Expression in Saccharomyces cerevisiae. |
| title_fullStr | Splicing-Mediated Autoregulation Modulates Rpl22p Expression in Saccharomyces cerevisiae. |
| title_full_unstemmed | Splicing-Mediated Autoregulation Modulates Rpl22p Expression in Saccharomyces cerevisiae. |
| title_short | Splicing-Mediated Autoregulation Modulates Rpl22p Expression in Saccharomyces cerevisiae. |
| title_sort | splicing mediated autoregulation modulates rpl22p expression in saccharomyces cerevisiae |
| url | http://europepmc.org/articles/PMC4838235?pdf=render |
| work_keys_str_mv | AT jasongabunilas splicingmediatedautoregulationmodulatesrpl22pexpressioninsaccharomycescerevisiae AT guillaumechanfreau splicingmediatedautoregulationmodulatesrpl22pexpressioninsaccharomycescerevisiae |