Detection and molecular analysis of <em>Hop latent virus</em> and <em>Hop latent viroid</em> in hop samples from Poland
Monitoring the occurrence of virus diseases in plants is important for the implementation of early control measures and prevention of further disease spread. In Poland, in 2004 a health programme for hop was started to eliminate viruses and viroids. In 2012/13, in vitro plants, samples from the IUNG...
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| Format: | Article |
| Language: | deu |
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Julius Kühn-Institut
2014-07-01
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| Series: | Journal für Kulturpflanzen |
| Subjects: | |
| Online Access: | https://ojs.openagrar.de/index.php/Kulturpflanzenjournal/article/view/12530 |
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| author | Angelika Ziegler Magdalena Kawka Marcin Przybys Teresa Doroszewska Urszula Skomra Ute Kastirr Jaroslav Matoušek Jörg Schubert |
| author_facet | Angelika Ziegler Magdalena Kawka Marcin Przybys Teresa Doroszewska Urszula Skomra Ute Kastirr Jaroslav Matoušek Jörg Schubert |
| author_sort | Angelika Ziegler |
| collection | DOAJ |
| description | Monitoring the occurrence of virus diseases in plants is important for the implementation of early control measures and prevention of further disease spread. In Poland, in 2004 a health programme for hop was started to eliminate viruses and viroids. In 2012/13, in vitro plants, samples from the IUNG-PIB experimental station and commercial hop gardens in Poland were tested for Hop latent virus (HpLV), and Hop latent and Hop stunt viroids (HpLVd and HpSVd). For virus testing, RT-PCR and ELISA methods were used. In order to detect hop viroids, RT-PCR was employed. The overall incidence of HpLV and hop viroids was lower than reported before the start of the programme. Cloning and sequencing revealed that the HpLV and the HpLVd from Polish sources are very similar to the type sequences and the Czech sources.
DOI: 10.5073/JfK.2014.07.04, https://doi.org/10.5073/JfK.2014.07.04 |
| first_indexed | 2024-12-13T01:18:45Z |
| format | Article |
| id | doaj.art-d066d91bb4924ce79586af9b441236f0 |
| institution | Directory Open Access Journal |
| issn | 1867-0911 1867-0938 |
| language | deu |
| last_indexed | 2024-12-13T01:18:45Z |
| publishDate | 2014-07-01 |
| publisher | Julius Kühn-Institut |
| record_format | Article |
| series | Journal für Kulturpflanzen |
| spelling | doaj.art-d066d91bb4924ce79586af9b441236f02022-12-22T00:04:17ZdeuJulius Kühn-InstitutJournal für Kulturpflanzen1867-09111867-09382014-07-01667Detection and molecular analysis of <em>Hop latent virus</em> and <em>Hop latent viroid</em> in hop samples from PolandAngelika Ziegler0Magdalena Kawka1Marcin Przybys2Teresa Doroszewska3Urszula Skomra4Ute Kastirr5Jaroslav Matoušek6Jörg Schubert7Institute for Epidemiology and Pathogen Diagnostics, Julius Kühn-Institut – Federal Research Centre for Cultivated Plants, Quedlinburg, GermanyInstitute of Soil Science and Plant Cultivation-State Research Institute, Department of Plant Breeding and Biotechnology, Pulawy, PolandInstitute of Soil Science and Plant Cultivation-State Research Institute, Department of Plant Breeding and Biotechnology, Pulawy, PolandInstitute of Soil Science and Plant Cultivation-State Research Institute, Department of Plant Breeding and Biotechnology, Pulawy, PolandInstitute of Soil Science and Plant Cultivation-State Research Institute, Department of Plant Breeding and Biotechnology, Pulawy, PolandInstitute for Epidemiology and Pathogen Diagnostics, Julius Kühn-Institut – Federal Research Centre for Cultivated Plants, Quedlinburg, GermanyBiol. Centre AS CR v.v.i., Institute of Plant Molecular Biology, 37005 České Budějovice, Czech RepublicInstitute for Biosafety in Plant Biotechnology, Julius Kühn-Institut – Federal Research Centre for Cultivated Plants, Quedlinburg, GermanyMonitoring the occurrence of virus diseases in plants is important for the implementation of early control measures and prevention of further disease spread. In Poland, in 2004 a health programme for hop was started to eliminate viruses and viroids. In 2012/13, in vitro plants, samples from the IUNG-PIB experimental station and commercial hop gardens in Poland were tested for Hop latent virus (HpLV), and Hop latent and Hop stunt viroids (HpLVd and HpSVd). For virus testing, RT-PCR and ELISA methods were used. In order to detect hop viroids, RT-PCR was employed. The overall incidence of HpLV and hop viroids was lower than reported before the start of the programme. Cloning and sequencing revealed that the HpLV and the HpLVd from Polish sources are very similar to the type sequences and the Czech sources. DOI: 10.5073/JfK.2014.07.04, https://doi.org/10.5073/JfK.2014.07.04https://ojs.openagrar.de/index.php/Kulturpflanzenjournal/article/view/12530Hop latent virushop latent viroidhop stunt viroidRT-PCRreal time RT-PCRELISA |
| spellingShingle | Angelika Ziegler Magdalena Kawka Marcin Przybys Teresa Doroszewska Urszula Skomra Ute Kastirr Jaroslav Matoušek Jörg Schubert Detection and molecular analysis of <em>Hop latent virus</em> and <em>Hop latent viroid</em> in hop samples from Poland Journal für Kulturpflanzen Hop latent virus hop latent viroid hop stunt viroid RT-PCR real time RT-PCR ELISA |
| title | Detection and molecular analysis of <em>Hop latent virus</em> and <em>Hop latent viroid</em> in hop samples from Poland |
| title_full | Detection and molecular analysis of <em>Hop latent virus</em> and <em>Hop latent viroid</em> in hop samples from Poland |
| title_fullStr | Detection and molecular analysis of <em>Hop latent virus</em> and <em>Hop latent viroid</em> in hop samples from Poland |
| title_full_unstemmed | Detection and molecular analysis of <em>Hop latent virus</em> and <em>Hop latent viroid</em> in hop samples from Poland |
| title_short | Detection and molecular analysis of <em>Hop latent virus</em> and <em>Hop latent viroid</em> in hop samples from Poland |
| title_sort | detection and molecular analysis of em hop latent virus em and em hop latent viroid em in hop samples from poland |
| topic | Hop latent virus hop latent viroid hop stunt viroid RT-PCR real time RT-PCR ELISA |
| url | https://ojs.openagrar.de/index.php/Kulturpflanzenjournal/article/view/12530 |
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