Integrating transcriptomics and metabolomics to analyze the mechanism of hypertension-induced hippocampal injury
ObjectiveHypertension is a public health challenge worldwide due to its high prevalence and multiple complications. Hypertension-induced damage to the hippocampus leads to behavioral changes and various brain diseases. Despite the multifaceted effects of hypertension on the hippocampus, the mechanis...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-04-01
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| Series: | Frontiers in Molecular Neuroscience |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fnmol.2023.1146525/full |
| _version_ | 1797851398631587840 |
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| author | Yanan Li Xue Chu Xin Xie Xin Xie Jinxiu Guo Junjun Meng Qingying Si Pei Jiang Pei Jiang |
| author_facet | Yanan Li Xue Chu Xin Xie Xin Xie Jinxiu Guo Junjun Meng Qingying Si Pei Jiang Pei Jiang |
| author_sort | Yanan Li |
| collection | DOAJ |
| description | ObjectiveHypertension is a public health challenge worldwide due to its high prevalence and multiple complications. Hypertension-induced damage to the hippocampus leads to behavioral changes and various brain diseases. Despite the multifaceted effects of hypertension on the hippocampus, the mechanisms underlying hippocampal lesions are still unclear.MethodsThe 32-week-old spontaneously hypertensive rats (SHR) and Wistar-Kyoto (WKY) rats were selected as the study subjects. Behavioral experiments such as an open field test (OFT), an elevated plus maze (EPM) test, and the Morris water maze (MWM) test were performed to show the behavioral characteristics of the rats. A comprehensive transcriptomic and metabolomic analysis was performed to understand the changes in the hippocampus at the metabolic and genetic levels.ResultsBehavioral tests showed that, compared to WKY rats, SHR showed not only reduced memory capacity but more hyperactive and impulsive behavior. In addition, transcriptomic analysis screened for 103 differentially expressed genes. Metabolomic analysis screened 56 metabolites with significant differences, including various amino acids and their related metabolites.ConclusionComprehensive analysis showed that hypertension-induced hippocampal lesions are closely associated with differential metabolites and differential genes detected in this study. The results provide a basis for analyzing the mechanisms of hypertension-induced hippocampal damage. |
| first_indexed | 2024-04-09T19:17:15Z |
| format | Article |
| id | doaj.art-d068065865c4480db2b236e0b646e15a |
| institution | Directory Open Access Journal |
| issn | 1662-5099 |
| language | English |
| last_indexed | 2024-04-09T19:17:15Z |
| publishDate | 2023-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Molecular Neuroscience |
| spelling | doaj.art-d068065865c4480db2b236e0b646e15a2023-04-06T05:05:56ZengFrontiers Media S.A.Frontiers in Molecular Neuroscience1662-50992023-04-011610.3389/fnmol.2023.11465251146525Integrating transcriptomics and metabolomics to analyze the mechanism of hypertension-induced hippocampal injuryYanan Li0Xue Chu1Xin Xie2Xin Xie3Jinxiu Guo4Junjun Meng5Qingying Si6Pei Jiang7Pei Jiang8Translational Pharmaceutical Laboratory, Jining First People’s Hospital, Shandong First Medical University, Jining, ChinaTranslational Pharmaceutical Laboratory, Jining First People’s Hospital, Shandong First Medical University, Jining, ChinaTranslational Pharmaceutical Laboratory, Jining First People’s Hospital, Shandong First Medical University, Jining, ChinaSchool of Pharmaceutical Sciences, Gannan Medical University, Ganzhou, ChinaTranslational Pharmaceutical Laboratory, Jining First People’s Hospital, Shandong First Medical University, Jining, ChinaTranslational Pharmaceutical Laboratory, Jining First People’s Hospital, Shandong First Medical University, Jining, ChinaDepartment of Endocrinology, Tengzhou Central People's Hospital, Tengzhou, ChinaTranslational Pharmaceutical Laboratory, Jining First People’s Hospital, Shandong First Medical University, Jining, ChinaInstitute of Translational Pharmacy, Jining Medical Research Academy, Jining, ChinaObjectiveHypertension is a public health challenge worldwide due to its high prevalence and multiple complications. Hypertension-induced damage to the hippocampus leads to behavioral changes and various brain diseases. Despite the multifaceted effects of hypertension on the hippocampus, the mechanisms underlying hippocampal lesions are still unclear.MethodsThe 32-week-old spontaneously hypertensive rats (SHR) and Wistar-Kyoto (WKY) rats were selected as the study subjects. Behavioral experiments such as an open field test (OFT), an elevated plus maze (EPM) test, and the Morris water maze (MWM) test were performed to show the behavioral characteristics of the rats. A comprehensive transcriptomic and metabolomic analysis was performed to understand the changes in the hippocampus at the metabolic and genetic levels.ResultsBehavioral tests showed that, compared to WKY rats, SHR showed not only reduced memory capacity but more hyperactive and impulsive behavior. In addition, transcriptomic analysis screened for 103 differentially expressed genes. Metabolomic analysis screened 56 metabolites with significant differences, including various amino acids and their related metabolites.ConclusionComprehensive analysis showed that hypertension-induced hippocampal lesions are closely associated with differential metabolites and differential genes detected in this study. The results provide a basis for analyzing the mechanisms of hypertension-induced hippocampal damage.https://www.frontiersin.org/articles/10.3389/fnmol.2023.1146525/fullspontaneously hypertensive ratshypertensionmetabolomicstranscriptomicshippocampus |
| spellingShingle | Yanan Li Xue Chu Xin Xie Xin Xie Jinxiu Guo Junjun Meng Qingying Si Pei Jiang Pei Jiang Integrating transcriptomics and metabolomics to analyze the mechanism of hypertension-induced hippocampal injury Frontiers in Molecular Neuroscience spontaneously hypertensive rats hypertension metabolomics transcriptomics hippocampus |
| title | Integrating transcriptomics and metabolomics to analyze the mechanism of hypertension-induced hippocampal injury |
| title_full | Integrating transcriptomics and metabolomics to analyze the mechanism of hypertension-induced hippocampal injury |
| title_fullStr | Integrating transcriptomics and metabolomics to analyze the mechanism of hypertension-induced hippocampal injury |
| title_full_unstemmed | Integrating transcriptomics and metabolomics to analyze the mechanism of hypertension-induced hippocampal injury |
| title_short | Integrating transcriptomics and metabolomics to analyze the mechanism of hypertension-induced hippocampal injury |
| title_sort | integrating transcriptomics and metabolomics to analyze the mechanism of hypertension induced hippocampal injury |
| topic | spontaneously hypertensive rats hypertension metabolomics transcriptomics hippocampus |
| url | https://www.frontiersin.org/articles/10.3389/fnmol.2023.1146525/full |
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