Genome-wide characterization of graft-transmissible mRNA-coding P450 genes of cucumber (Cucumis sativus L.)
P450 (cytochrome P450) is a supergene family, which is involved in various metabolic pathways in plants. Based on previous study, we found some of cucumber P450 mRNAs were systemic mobile in cucumber/pumpkin grafts. However, the reason that why P450 mRNAs were endorsed as signaling, and what specifi...
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| Format: | Article |
| Language: | English |
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KeAi Communications Co., Ltd.
2023-04-01
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| Series: | Horticultural Plant Journal |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2468014122000267 |
| _version_ | 1797847728271654912 |
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| author | Tao Wang Xiaohong Lu Chenggang Xiang Xiaojun Li Wenqian Liu Qing Wang Zixi Liu Lihong Gao Wenna Zhang |
| author_facet | Tao Wang Xiaohong Lu Chenggang Xiang Xiaojun Li Wenqian Liu Qing Wang Zixi Liu Lihong Gao Wenna Zhang |
| author_sort | Tao Wang |
| collection | DOAJ |
| description | P450 (cytochrome P450) is a supergene family, which is involved in various metabolic pathways in plants. Based on previous study, we found some of cucumber P450 mRNAs were systemic mobile in cucumber/pumpkin grafts. However, the reason that why P450 mRNAs were endorsed as signaling, and what specific motif (s) did they harbored is not clear yet. Here, we first identified 221 CsaP450 genes in cucumber genome-wide level. Combining with graft-transmissible mRNAs datasets in cucumber, we elucidated 15 mobile-CsaP450-coding genes, of which 5 and 10 belonged to A-type and non-A type respectively. Compared with Arabidopsis and pumpkin (Cucubit moschata) graft-induced-transmissible P450 mRNAs, a phylogenetic tree was constructed and divided into eight clans by using multiple-sequence alignment. Gene ontology (GO) enrichment and Kyoto Encyclopedia of Genes and Genomes (KEGG) annotations indicated that the expression patterns of the mobile-mRNA-coding CsaP450 genes in different tissues of cucumber was specifically enriched in oxidoreductase activity and secondary metabolic pathways. The structures and motifs of these 15 mobile-mRNA-coding CsaP450 genes and their types of regulatory elements told that the proportion of CU-rich motifs was higher than nonmobile-mRNA-coding CsaP450 genes. The integrated analysis of mobility direction and mRNA abundance of 15 mobile-mRNA-coding CsaP450 genes allowed to conclude that there was rarely relationship between them. The study provided a new insight into the relationship between the motifs and functional characterization of mobile-mRNA-coding P450 genes of cucumber in genome-wide levels. |
| first_indexed | 2024-04-09T18:16:04Z |
| format | Article |
| id | doaj.art-d069592832df499d881951e06596514b |
| institution | Directory Open Access Journal |
| issn | 2468-0141 |
| language | English |
| last_indexed | 2024-04-09T18:16:04Z |
| publishDate | 2023-04-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Horticultural Plant Journal |
| spelling | doaj.art-d069592832df499d881951e06596514b2023-04-13T04:26:56ZengKeAi Communications Co., Ltd.Horticultural Plant Journal2468-01412023-04-0192250260Genome-wide characterization of graft-transmissible mRNA-coding P450 genes of cucumber (Cucumis sativus L.)Tao Wang0Xiaohong Lu1Chenggang Xiang2Xiaojun Li3Wenqian Liu4Qing Wang5Zixi Liu6Lihong Gao7Wenna Zhang8Beijing Key Laboratory of Growth and Developmental Regulation for Protected Vegetable Crops, China Agricultural University, Beijing, 100193 ChinaBeijing Key Laboratory of Growth and Developmental Regulation for Protected Vegetable Crops, China Agricultural University, Beijing, 100193 ChinaCollege of Life Science and Technology, HongHe University, Mengzi, Yunnan, 661199 ChinaBeijing Key Laboratory of Growth and Developmental Regulation for Protected Vegetable Crops, China Agricultural University, Beijing, 100193 ChinaBeijing Key Laboratory of Growth and Developmental Regulation for Protected Vegetable Crops, China Agricultural University, Beijing, 100193 ChinaBeijing Key Laboratory of Growth and Developmental Regulation for Protected Vegetable Crops, China Agricultural University, Beijing, 100193 ChinaBeijing Key Laboratory of Growth and Developmental Regulation for Protected Vegetable Crops, China Agricultural University, Beijing, 100193 ChinaBeijing Key Laboratory of Growth and Developmental Regulation for Protected Vegetable Crops, China Agricultural University, Beijing, 100193 ChinaBeijing Key Laboratory of Growth and Developmental Regulation for Protected Vegetable Crops, China Agricultural University, Beijing, 100193 China; Corresponding author.P450 (cytochrome P450) is a supergene family, which is involved in various metabolic pathways in plants. Based on previous study, we found some of cucumber P450 mRNAs were systemic mobile in cucumber/pumpkin grafts. However, the reason that why P450 mRNAs were endorsed as signaling, and what specific motif (s) did they harbored is not clear yet. Here, we first identified 221 CsaP450 genes in cucumber genome-wide level. Combining with graft-transmissible mRNAs datasets in cucumber, we elucidated 15 mobile-CsaP450-coding genes, of which 5 and 10 belonged to A-type and non-A type respectively. Compared with Arabidopsis and pumpkin (Cucubit moschata) graft-induced-transmissible P450 mRNAs, a phylogenetic tree was constructed and divided into eight clans by using multiple-sequence alignment. Gene ontology (GO) enrichment and Kyoto Encyclopedia of Genes and Genomes (KEGG) annotations indicated that the expression patterns of the mobile-mRNA-coding CsaP450 genes in different tissues of cucumber was specifically enriched in oxidoreductase activity and secondary metabolic pathways. The structures and motifs of these 15 mobile-mRNA-coding CsaP450 genes and their types of regulatory elements told that the proportion of CU-rich motifs was higher than nonmobile-mRNA-coding CsaP450 genes. The integrated analysis of mobility direction and mRNA abundance of 15 mobile-mRNA-coding CsaP450 genes allowed to conclude that there was rarely relationship between them. The study provided a new insight into the relationship between the motifs and functional characterization of mobile-mRNA-coding P450 genes of cucumber in genome-wide levels.http://www.sciencedirect.com/science/article/pii/S2468014122000267Cucumis sativusGenome-wideCytochrome P450mRNA mobilityPhylogenyCU-rich motif |
| spellingShingle | Tao Wang Xiaohong Lu Chenggang Xiang Xiaojun Li Wenqian Liu Qing Wang Zixi Liu Lihong Gao Wenna Zhang Genome-wide characterization of graft-transmissible mRNA-coding P450 genes of cucumber (Cucumis sativus L.) Horticultural Plant Journal Cucumis sativus Genome-wide Cytochrome P450 mRNA mobility Phylogeny CU-rich motif |
| title | Genome-wide characterization of graft-transmissible mRNA-coding P450 genes of cucumber (Cucumis sativus L.) |
| title_full | Genome-wide characterization of graft-transmissible mRNA-coding P450 genes of cucumber (Cucumis sativus L.) |
| title_fullStr | Genome-wide characterization of graft-transmissible mRNA-coding P450 genes of cucumber (Cucumis sativus L.) |
| title_full_unstemmed | Genome-wide characterization of graft-transmissible mRNA-coding P450 genes of cucumber (Cucumis sativus L.) |
| title_short | Genome-wide characterization of graft-transmissible mRNA-coding P450 genes of cucumber (Cucumis sativus L.) |
| title_sort | genome wide characterization of graft transmissible mrna coding p450 genes of cucumber cucumis sativus l |
| topic | Cucumis sativus Genome-wide Cytochrome P450 mRNA mobility Phylogeny CU-rich motif |
| url | http://www.sciencedirect.com/science/article/pii/S2468014122000267 |
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