Pramef12 enhances reprogramming into naïve iPS cells
Somatic cells can be reprogrammed into induced pluripotent stem (iPS) cells by forced expression of the transcription factors Oct3/4, Klf4, Sox2, and c-Myc (OKSM). Somatic cell nuclear transfer can also be utilized to reprogram somatic cells into totipotent embryos, suggesting that factors present i...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-07-01
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| Series: | Biochemistry and Biophysics Reports |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S240558082200067X |
| _version_ | 1817977468321005568 |
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| author | Daiki Haraguchi Toshinobu Nakamura |
| author_facet | Daiki Haraguchi Toshinobu Nakamura |
| author_sort | Daiki Haraguchi |
| collection | DOAJ |
| description | Somatic cells can be reprogrammed into induced pluripotent stem (iPS) cells by forced expression of the transcription factors Oct3/4, Klf4, Sox2, and c-Myc (OKSM). Somatic cell nuclear transfer can also be utilized to reprogram somatic cells into totipotent embryos, suggesting that factors present in oocytes potentially enhance the efficiency of iPS cell generation. Here, we showed that preferentially expressed antigen of melanoma family member 12 (Pramef12), which is highly expressed in oocytes, enhances the generation of iPS cells from mouse fibroblasts. Overexpression of Pramef12 during the early phase of OKSM-induced reprogramming enhanced the efficiency of iPS cell derivation. In addition, overexpression of Pramef12 also enhanced expression of naïve pluripotency-associated genes, Gtl2 located within the Dlk1–Dio3 imprinted region essential for full pluripotency, glycolysis-associated genes, and oxidative phosphorylation-associated genes, and it promoted mesenchymal-to-epithelial transition during iPS cell generation. Furthermore, Pramef12 greatly activated β-catenin during iPS cell generation. These observations suggested that Pramef12 enhances OKSM-induced reprogramming via activation of the Wnt/β-catenin pathway. |
| first_indexed | 2024-04-13T22:16:51Z |
| format | Article |
| id | doaj.art-d06af630ee79469e9983f5db429a8784 |
| institution | Directory Open Access Journal |
| issn | 2405-5808 |
| language | English |
| last_indexed | 2024-04-13T22:16:51Z |
| publishDate | 2022-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biochemistry and Biophysics Reports |
| spelling | doaj.art-d06af630ee79469e9983f5db429a87842022-12-22T02:27:30ZengElsevierBiochemistry and Biophysics Reports2405-58082022-07-0130101267Pramef12 enhances reprogramming into naïve iPS cellsDaiki Haraguchi0Toshinobu Nakamura1Gaduate School of Bio-Science, JapanGaduate School of Bio-Science, Japan; Department of Bio-Science, Japan; Genome Editing Research Institute, Ngahama Institute of Bio-Science and Technology, Shiga, 526-0829, Japan; Corresponding author. Laboratory for epigenetic regulation, Department of Bio-Science, Nagahama Institute of Bio-Science and Technology, Japan.Somatic cells can be reprogrammed into induced pluripotent stem (iPS) cells by forced expression of the transcription factors Oct3/4, Klf4, Sox2, and c-Myc (OKSM). Somatic cell nuclear transfer can also be utilized to reprogram somatic cells into totipotent embryos, suggesting that factors present in oocytes potentially enhance the efficiency of iPS cell generation. Here, we showed that preferentially expressed antigen of melanoma family member 12 (Pramef12), which is highly expressed in oocytes, enhances the generation of iPS cells from mouse fibroblasts. Overexpression of Pramef12 during the early phase of OKSM-induced reprogramming enhanced the efficiency of iPS cell derivation. In addition, overexpression of Pramef12 also enhanced expression of naïve pluripotency-associated genes, Gtl2 located within the Dlk1–Dio3 imprinted region essential for full pluripotency, glycolysis-associated genes, and oxidative phosphorylation-associated genes, and it promoted mesenchymal-to-epithelial transition during iPS cell generation. Furthermore, Pramef12 greatly activated β-catenin during iPS cell generation. These observations suggested that Pramef12 enhances OKSM-induced reprogramming via activation of the Wnt/β-catenin pathway.http://www.sciencedirect.com/science/article/pii/S240558082200067XiPS cellsReprogrammingPramef12Wnt/β-catenin pathway |
| spellingShingle | Daiki Haraguchi Toshinobu Nakamura Pramef12 enhances reprogramming into naïve iPS cells Biochemistry and Biophysics Reports iPS cells Reprogramming Pramef12 Wnt/β-catenin pathway |
| title | Pramef12 enhances reprogramming into naïve iPS cells |
| title_full | Pramef12 enhances reprogramming into naïve iPS cells |
| title_fullStr | Pramef12 enhances reprogramming into naïve iPS cells |
| title_full_unstemmed | Pramef12 enhances reprogramming into naïve iPS cells |
| title_short | Pramef12 enhances reprogramming into naïve iPS cells |
| title_sort | pramef12 enhances reprogramming into naive ips cells |
| topic | iPS cells Reprogramming Pramef12 Wnt/β-catenin pathway |
| url | http://www.sciencedirect.com/science/article/pii/S240558082200067X |
| work_keys_str_mv | AT daikiharaguchi pramef12enhancesreprogrammingintonaiveipscells AT toshinobunakamura pramef12enhancesreprogrammingintonaiveipscells |