Ram semen deterioration by short-term exposure to high altitude is prevented by improvement of antioxidant status
Ovine reproduction efficiency in herds at high altitude (ha) is lower than that at low altitude (la). In ewes, ha effects are due to hypoxia and oxidative stress. Our aim was to establish the effect of antioxidant vitamin supplementation on semen traits and antioxidant status of rams exposed to shor...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2018-01-01
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| Series: | Animal |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1751731117002452 |
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| author | E. Cofré O.A. Peralta A. Raggi M. De los Reyes F. Sales A. González-Bulnes V.H. Parraguez |
| author_facet | E. Cofré O.A. Peralta A. Raggi M. De los Reyes F. Sales A. González-Bulnes V.H. Parraguez |
| author_sort | E. Cofré |
| collection | DOAJ |
| description | Ovine reproduction efficiency in herds at high altitude (ha) is lower than that at low altitude (la). In ewes, ha effects are due to hypoxia and oxidative stress. Our aim was to establish the effect of antioxidant vitamin supplementation on semen traits and antioxidant status of rams exposed to short or long time ha. A total of 32 rams native to la (~500 m) were used, 16 were kept at la and the other 16 were brought to ha (~3600 m), where they were placed in the same flock as the ha native rams (n=16). Half of the animals in each group were supplemented daily with vitamins C 600 mg and E 450 IU per os, during the entire experimental period, starting the 4th day after animal’s arrival at ha (day 0). At days 0, 30 and 60 of treatment, blood and semen samples were collected for evaluation of antioxidant status and semen standard characteristics. Data were compared within each experimental time by analysis of variance using a general linear model. Elevated concentrations of oxidative stress biomarkers were present in blood from animals maintained at ha. Ejaculates from ha exposed rams showed decreased sperm concentration, progressive motility and viability, in addition to decreased antioxidant status in seminal fluid. A total of 30 days of oral supplementation with vitamins C and E prevented some ha negative effects on semen characteristics, mainly in recently ha exposed rams. It is concluded that exposure of rams to ha negatively affects semen quality, where oxidative stress plays a predominant role. These effects are mainly prevented by oral supplementation of vitamins C and E, which constitutes a simple and cheap alternative to improve semen quality of rams when they are moved to ha. |
| first_indexed | 2024-12-14T14:01:12Z |
| format | Article |
| id | doaj.art-d06d7201a4014e128f2922e79c8e234a |
| institution | Directory Open Access Journal |
| issn | 1751-7311 |
| language | English |
| last_indexed | 2024-12-14T14:01:12Z |
| publishDate | 2018-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Animal |
| spelling | doaj.art-d06d7201a4014e128f2922e79c8e234a2022-12-21T22:58:43ZengElsevierAnimal1751-73112018-01-0112510071014Ram semen deterioration by short-term exposure to high altitude is prevented by improvement of antioxidant statusE. Cofré0O.A. Peralta1A. Raggi2M. De los Reyes3F. Sales4A. González-Bulnes5V.H. Parraguez6Faculty of Veterinary Sciences, University of Chile, Santa Rosa 11735, 8820808 Santiago, ChileFaculty of Veterinary Sciences, University of Chile, Santa Rosa 11735, 8820808 Santiago, ChileFaculty of Agricultural Sciences, University of Chile, Santa Rosa 11315, 8820808 Santiago, Chile; International Centre for Andean Studies, University of Chile, Santa Rosa 11735, 8820808 Santiago, ChileFaculty of Veterinary Sciences, University of Chile, Santa Rosa 11735, 8820808 Santiago, ChileDepartment of Animal Science, INIA-Kampenaike, Angamos 1056, 6212707 Punta Arenas, ChileDepartment of Animal Reproduction, INIA, Av. Puerta de Hierro s/n, 28040 Madrid, Spain; Department of Veterinary Medicine, University of Sassari, Piazza Università 21 Sassari, ItalyFaculty of Veterinary Sciences, University of Chile, Santa Rosa 11735, 8820808 Santiago, Chile; Faculty of Agricultural Sciences, University of Chile, Santa Rosa 11315, 8820808 Santiago, Chile; International Centre for Andean Studies, University of Chile, Santa Rosa 11735, 8820808 Santiago, ChileOvine reproduction efficiency in herds at high altitude (ha) is lower than that at low altitude (la). In ewes, ha effects are due to hypoxia and oxidative stress. Our aim was to establish the effect of antioxidant vitamin supplementation on semen traits and antioxidant status of rams exposed to short or long time ha. A total of 32 rams native to la (~500 m) were used, 16 were kept at la and the other 16 were brought to ha (~3600 m), where they were placed in the same flock as the ha native rams (n=16). Half of the animals in each group were supplemented daily with vitamins C 600 mg and E 450 IU per os, during the entire experimental period, starting the 4th day after animal’s arrival at ha (day 0). At days 0, 30 and 60 of treatment, blood and semen samples were collected for evaluation of antioxidant status and semen standard characteristics. Data were compared within each experimental time by analysis of variance using a general linear model. Elevated concentrations of oxidative stress biomarkers were present in blood from animals maintained at ha. Ejaculates from ha exposed rams showed decreased sperm concentration, progressive motility and viability, in addition to decreased antioxidant status in seminal fluid. A total of 30 days of oral supplementation with vitamins C and E prevented some ha negative effects on semen characteristics, mainly in recently ha exposed rams. It is concluded that exposure of rams to ha negatively affects semen quality, where oxidative stress plays a predominant role. These effects are mainly prevented by oral supplementation of vitamins C and E, which constitutes a simple and cheap alternative to improve semen quality of rams when they are moved to ha.http://www.sciencedirect.com/science/article/pii/S1751731117002452ram semenhigh altitudeoxidative stressantioxidant vitamins |
| spellingShingle | E. Cofré O.A. Peralta A. Raggi M. De los Reyes F. Sales A. González-Bulnes V.H. Parraguez Ram semen deterioration by short-term exposure to high altitude is prevented by improvement of antioxidant status Animal ram semen high altitude oxidative stress antioxidant vitamins |
| title | Ram semen deterioration by short-term exposure to high altitude is prevented by improvement of antioxidant status |
| title_full | Ram semen deterioration by short-term exposure to high altitude is prevented by improvement of antioxidant status |
| title_fullStr | Ram semen deterioration by short-term exposure to high altitude is prevented by improvement of antioxidant status |
| title_full_unstemmed | Ram semen deterioration by short-term exposure to high altitude is prevented by improvement of antioxidant status |
| title_short | Ram semen deterioration by short-term exposure to high altitude is prevented by improvement of antioxidant status |
| title_sort | ram semen deterioration by short term exposure to high altitude is prevented by improvement of antioxidant status |
| topic | ram semen high altitude oxidative stress antioxidant vitamins |
| url | http://www.sciencedirect.com/science/article/pii/S1751731117002452 |
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