Circulating cell free DNA response to exhaustive exercise in average trained men with type I diabetes mellitus
Abstract It is believed that neutrophils extracellular traps (NETs) formation is responsible for the increase in cf DNA after exercise. Since T1DM is accompanied by enhanced NETs generation, we compared exercise-induced increase in cf DNA in 14 men with T1DM and 11 healthy controls and analyzed its...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2021-02-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-021-84201-0 |
| _version_ | 1818996308652851200 |
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| author | Konrad Walczak Robert Stawski Ewelina Perdas Olga Brzezinska Piotr Kosielski Szymon Galczynski Tomasz Budlewski Gianluca Padula Dariusz Nowak |
| author_facet | Konrad Walczak Robert Stawski Ewelina Perdas Olga Brzezinska Piotr Kosielski Szymon Galczynski Tomasz Budlewski Gianluca Padula Dariusz Nowak |
| author_sort | Konrad Walczak |
| collection | DOAJ |
| description | Abstract It is believed that neutrophils extracellular traps (NETs) formation is responsible for the increase in cf DNA after exercise. Since T1DM is accompanied by enhanced NETs generation, we compared exercise-induced increase in cf DNA in 14 men with T1DM and 11 healthy controls and analyzed its association with exercise load. Subjects performed a treadmill run to exhaustion at speed corresponding to 70% of their personal VO2max. Blood was collected before and just after exercise for determination of plasma cf nuclear and mitochondrial DNA (cf n-DNA, cf mt-DNA) by real-time PCR, blood cell count and metabolic markers. Exercise resulted in the increase in median cf n-DNA from 3.9 ng/mL to 21.0 ng/mL in T1DM group and from 3.3 ng/mL to 28.9 ng/mL in controls. Median exercise-induced increment (∆) in cf n-DNA did not differ significantly in both groups (17.8 ng/mL vs. 22.1 ng/mL, p = 0.23), but this variable correlated with run distance (r = 0.66), Δ neutrophils (r = 0.86), Δ creatinine (r = 0.65) and Δ creatine kinase (r = 0.77) only in controls. Pre- and post-exercise cf mt-DNA were not significantly different within and between groups. These suggest low usefulness of Δ cf n-DNA as a marker of exercise intensity in T1DM men. |
| first_indexed | 2024-12-20T21:27:41Z |
| format | Article |
| id | doaj.art-d06f130f80974e44958c3a6388e25aed |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-20T21:27:41Z |
| publishDate | 2021-02-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-d06f130f80974e44958c3a6388e25aed2022-12-21T19:26:07ZengNature PortfolioScientific Reports2045-23222021-02-0111111210.1038/s41598-021-84201-0Circulating cell free DNA response to exhaustive exercise in average trained men with type I diabetes mellitusKonrad Walczak0Robert Stawski1Ewelina Perdas2Olga Brzezinska3Piotr Kosielski4Szymon Galczynski5Tomasz Budlewski6Gianluca Padula7Dariusz Nowak8Department of Internal Medicine and Nephrodiabetology, Medical University of LodzDepartment of Clinical Physiology, Medical University of LodzDepartment of Clinical Physiology, Medical University of LodzDepartment of Rheumatology, Medical University of LodzAcademic Laboratory of Movement and Human Physical Performance, Medical University of LodzAcademic Laboratory of Movement and Human Physical Performance, Medical University of LodzDepartment of Rheumatology, Medical University of LodzAcademic Laboratory of Movement and Human Physical Performance, Medical University of LodzDepartment of Clinical Physiology, Medical University of LodzAbstract It is believed that neutrophils extracellular traps (NETs) formation is responsible for the increase in cf DNA after exercise. Since T1DM is accompanied by enhanced NETs generation, we compared exercise-induced increase in cf DNA in 14 men with T1DM and 11 healthy controls and analyzed its association with exercise load. Subjects performed a treadmill run to exhaustion at speed corresponding to 70% of their personal VO2max. Blood was collected before and just after exercise for determination of plasma cf nuclear and mitochondrial DNA (cf n-DNA, cf mt-DNA) by real-time PCR, blood cell count and metabolic markers. Exercise resulted in the increase in median cf n-DNA from 3.9 ng/mL to 21.0 ng/mL in T1DM group and from 3.3 ng/mL to 28.9 ng/mL in controls. Median exercise-induced increment (∆) in cf n-DNA did not differ significantly in both groups (17.8 ng/mL vs. 22.1 ng/mL, p = 0.23), but this variable correlated with run distance (r = 0.66), Δ neutrophils (r = 0.86), Δ creatinine (r = 0.65) and Δ creatine kinase (r = 0.77) only in controls. Pre- and post-exercise cf mt-DNA were not significantly different within and between groups. These suggest low usefulness of Δ cf n-DNA as a marker of exercise intensity in T1DM men.https://doi.org/10.1038/s41598-021-84201-0 |
| spellingShingle | Konrad Walczak Robert Stawski Ewelina Perdas Olga Brzezinska Piotr Kosielski Szymon Galczynski Tomasz Budlewski Gianluca Padula Dariusz Nowak Circulating cell free DNA response to exhaustive exercise in average trained men with type I diabetes mellitus Scientific Reports |
| title | Circulating cell free DNA response to exhaustive exercise in average trained men with type I diabetes mellitus |
| title_full | Circulating cell free DNA response to exhaustive exercise in average trained men with type I diabetes mellitus |
| title_fullStr | Circulating cell free DNA response to exhaustive exercise in average trained men with type I diabetes mellitus |
| title_full_unstemmed | Circulating cell free DNA response to exhaustive exercise in average trained men with type I diabetes mellitus |
| title_short | Circulating cell free DNA response to exhaustive exercise in average trained men with type I diabetes mellitus |
| title_sort | circulating cell free dna response to exhaustive exercise in average trained men with type i diabetes mellitus |
| url | https://doi.org/10.1038/s41598-021-84201-0 |
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