Electrochemical DNA-nano biosensor for the detection of cervical cancer-causing HPV-16 using ultrasmall Fe3O4-Au core-shell nanoparticles
This paper reports a label-free biosensor for detecting human papillomavirus type 16 (HPV-16). For this purpose, the surface of the screen-printed carbon electrodes (SPCEs) was coated with Fe3O4-Au core-shell nanoparticles (NPs) using a green and facile eco-friendly method. The modified surfaces of...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-06-01
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| Series: | Sensing and Bio-Sensing Research |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2214180423000144 |
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| author | Elisa Rasouli Wan Jefrey Basirun Mohd Rafie Johan Majid Rezayi Mohammad Reza Mahmoudian Daniel Puiu Poenar |
| author_facet | Elisa Rasouli Wan Jefrey Basirun Mohd Rafie Johan Majid Rezayi Mohammad Reza Mahmoudian Daniel Puiu Poenar |
| author_sort | Elisa Rasouli |
| collection | DOAJ |
| description | This paper reports a label-free biosensor for detecting human papillomavirus type 16 (HPV-16). For this purpose, the surface of the screen-printed carbon electrodes (SPCEs) was coated with Fe3O4-Au core-shell nanoparticles (NPs) using a green and facile eco-friendly method. The modified surfaces of the electrodes were then functionalized with thiolated single-strand DNA (ssDNA) probe human papillomavirus (HPV) DNA sequences. Next, the hybridization events with the immobilized probe DNA were monitored by cyclic voltammetry (CV) and differential pulse voltammetry (DPV) using [Fe(CN)6]3‐/4− as the redox indicator. Our results demonstrate that the modified electrodes could distinguish the redox current signals of [Fe(CN)6] 3−/4− due to the absence/presence of the immobilized probe DNA. Furthermore, quantitative estimations of the concentration of the probe DNA were also possible. Optimal performance was obtained for probe DNA concentrations between 1 and 10 μM. The best performance of our HPV biosensor was obtained for probe DNA concentration of 5 μM, for which the limit of detection and sensitivity of our developed sensor resulted to be 0.1 nM and 2.4 μA/nM, respectively. |
| first_indexed | 2024-03-13T09:12:34Z |
| format | Article |
| id | doaj.art-d06fa2abc9e74a73bb97d0a2783f27b7 |
| institution | Directory Open Access Journal |
| issn | 2214-1804 |
| language | English |
| last_indexed | 2024-03-13T09:12:34Z |
| publishDate | 2023-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Sensing and Bio-Sensing Research |
| spelling | doaj.art-d06fa2abc9e74a73bb97d0a2783f27b72023-05-27T04:25:17ZengElsevierSensing and Bio-Sensing Research2214-18042023-06-0140100562Electrochemical DNA-nano biosensor for the detection of cervical cancer-causing HPV-16 using ultrasmall Fe3O4-Au core-shell nanoparticlesElisa Rasouli0Wan Jefrey Basirun1Mohd Rafie Johan2Majid Rezayi3Mohammad Reza Mahmoudian4Daniel Puiu Poenar5Continental-NTU Corporate Lab, Nanyang Technological University, 50 Nanyang Avenue, 639798, Singapore; Corresponding author.Nanotechnology & Catalysis Research Centre, Institute of Postgraduate Studies, University Malaya, 50603 Kuala Lumpur, Malaysia.; Department of Chemistry, Faculty of Science, University Malaya, Kuala Lumpur 50603, MalaysiaNanotechnology & Catalysis Research Centre, Institute of Postgraduate Studies, University Malaya, 50603 Kuala Lumpur, Malaysia.Department of Medical Biotechnology and Nanotechnology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, IranDepartment of Chemistry, University of Farhangian, Tehran, IranContinental-NTU Corporate Lab, Nanyang Technological University, 50 Nanyang Avenue, 639798, SingaporeThis paper reports a label-free biosensor for detecting human papillomavirus type 16 (HPV-16). For this purpose, the surface of the screen-printed carbon electrodes (SPCEs) was coated with Fe3O4-Au core-shell nanoparticles (NPs) using a green and facile eco-friendly method. The modified surfaces of the electrodes were then functionalized with thiolated single-strand DNA (ssDNA) probe human papillomavirus (HPV) DNA sequences. Next, the hybridization events with the immobilized probe DNA were monitored by cyclic voltammetry (CV) and differential pulse voltammetry (DPV) using [Fe(CN)6]3‐/4− as the redox indicator. Our results demonstrate that the modified electrodes could distinguish the redox current signals of [Fe(CN)6] 3−/4− due to the absence/presence of the immobilized probe DNA. Furthermore, quantitative estimations of the concentration of the probe DNA were also possible. Optimal performance was obtained for probe DNA concentrations between 1 and 10 μM. The best performance of our HPV biosensor was obtained for probe DNA concentration of 5 μM, for which the limit of detection and sensitivity of our developed sensor resulted to be 0.1 nM and 2.4 μA/nM, respectively.http://www.sciencedirect.com/science/article/pii/S2214180423000144Fe3O4-au core-shell nanoparticlesModified screen-printed carbon electrodesDNA biosensorLabel-freeHuman papillomavirusCervical cancer |
| spellingShingle | Elisa Rasouli Wan Jefrey Basirun Mohd Rafie Johan Majid Rezayi Mohammad Reza Mahmoudian Daniel Puiu Poenar Electrochemical DNA-nano biosensor for the detection of cervical cancer-causing HPV-16 using ultrasmall Fe3O4-Au core-shell nanoparticles Sensing and Bio-Sensing Research Fe3O4-au core-shell nanoparticles Modified screen-printed carbon electrodes DNA biosensor Label-free Human papillomavirus Cervical cancer |
| title | Electrochemical DNA-nano biosensor for the detection of cervical cancer-causing HPV-16 using ultrasmall Fe3O4-Au core-shell nanoparticles |
| title_full | Electrochemical DNA-nano biosensor for the detection of cervical cancer-causing HPV-16 using ultrasmall Fe3O4-Au core-shell nanoparticles |
| title_fullStr | Electrochemical DNA-nano biosensor for the detection of cervical cancer-causing HPV-16 using ultrasmall Fe3O4-Au core-shell nanoparticles |
| title_full_unstemmed | Electrochemical DNA-nano biosensor for the detection of cervical cancer-causing HPV-16 using ultrasmall Fe3O4-Au core-shell nanoparticles |
| title_short | Electrochemical DNA-nano biosensor for the detection of cervical cancer-causing HPV-16 using ultrasmall Fe3O4-Au core-shell nanoparticles |
| title_sort | electrochemical dna nano biosensor for the detection of cervical cancer causing hpv 16 using ultrasmall fe3o4 au core shell nanoparticles |
| topic | Fe3O4-au core-shell nanoparticles Modified screen-printed carbon electrodes DNA biosensor Label-free Human papillomavirus Cervical cancer |
| url | http://www.sciencedirect.com/science/article/pii/S2214180423000144 |
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