Identification and genetic characterization of a novel parvovirus associated with serum hepatitis in horses in China
Abstract A novel equine parvovirus, equine parvovirus-hepatitis (EqPV-H), was first discovered in a horse that died of equine serum hepatitis in the USA in 2018. EqPV-H was shown to be a novel etiological agent associated with equine serum hepatitis. Following this initial report, no additional stud...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2018-10-01
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| Series: | Emerging Microbes and Infections |
| Online Access: | http://link.springer.com/article/10.1038/s41426-018-0174-2 |
| _version_ | 1828893484553076736 |
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| author | Gang Lu Lingshuang Sun Jiajun Ou Haibin Xu Liyan Wu Shoujun Li |
| author_facet | Gang Lu Lingshuang Sun Jiajun Ou Haibin Xu Liyan Wu Shoujun Li |
| author_sort | Gang Lu |
| collection | DOAJ |
| description | Abstract A novel equine parvovirus, equine parvovirus-hepatitis (EqPV-H), was first discovered in a horse that died of equine serum hepatitis in the USA in 2018. EqPV-H was shown to be a novel etiological agent associated with equine serum hepatitis. Following this initial report, no additional studies on EqPV-H have been published. In this study, a total of 143 serum samples were collected from racehorses at 5 separate farms in China and were analyzed to detect EqPV-H DNA via nested PCR. The results indicated a high prevalence of EqPV-H (11.9%, 17/143) in the studied animals. In addition, a remarkably high coinfection rate (58.8%, 10/17) with 2 equine flaviviruses (equine hepacivirus and equine pegivirus) was observed in the EqPV-H positive equines. However, all equines tested negative for Theiler’s disease-associated virus, an etiological agent associated with equine serum hepatitis. The genomes of six field EqPV-H strains were sequenced and analyzed, with the results indicating that the Chinese EqPV-H strains have low genetic diversity and high genetic similarity with the USA EqPV-H strain BCT-01. A phylogenetic analysis demonstrated that the Chinese EqPV-H strains clustered with BCT-01 in the genus Copiparvovirus but were distantly related to another equine parvovirus identified in horse cerebrospinal fluid. In addition, liver enzyme levels were detected in the EqPV-H positive serum samples, and all the values were in the normal range, indicating that infection can occur without concurrent liver disease. This study will promote an understanding of the geographical distribution, genetic diversity, and pathogenicity of EqPV-H. |
| first_indexed | 2024-12-13T13:55:16Z |
| format | Article |
| id | doaj.art-d07012ce46ad407497837a07b071fd90 |
| institution | Directory Open Access Journal |
| issn | 2222-1751 |
| language | English |
| last_indexed | 2024-12-13T13:55:16Z |
| publishDate | 2018-10-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Emerging Microbes and Infections |
| spelling | doaj.art-d07012ce46ad407497837a07b071fd902022-12-21T23:42:56ZengTaylor & Francis GroupEmerging Microbes and Infections2222-17512018-10-01711710.1038/s41426-018-0174-2Identification and genetic characterization of a novel parvovirus associated with serum hepatitis in horses in ChinaGang Lu0Lingshuang Sun1Jiajun Ou2Haibin Xu3Liyan Wu4Shoujun Li5College of Veterinary Medicine, South China Agricultural UniversityCollege of Veterinary Medicine, South China Agricultural UniversityCollege of Veterinary Medicine, South China Agricultural UniversityCollege of Veterinary Medicine, South China Agricultural UniversityCollege of Veterinary Medicine, South China Agricultural UniversityCollege of Veterinary Medicine, South China Agricultural UniversityAbstract A novel equine parvovirus, equine parvovirus-hepatitis (EqPV-H), was first discovered in a horse that died of equine serum hepatitis in the USA in 2018. EqPV-H was shown to be a novel etiological agent associated with equine serum hepatitis. Following this initial report, no additional studies on EqPV-H have been published. In this study, a total of 143 serum samples were collected from racehorses at 5 separate farms in China and were analyzed to detect EqPV-H DNA via nested PCR. The results indicated a high prevalence of EqPV-H (11.9%, 17/143) in the studied animals. In addition, a remarkably high coinfection rate (58.8%, 10/17) with 2 equine flaviviruses (equine hepacivirus and equine pegivirus) was observed in the EqPV-H positive equines. However, all equines tested negative for Theiler’s disease-associated virus, an etiological agent associated with equine serum hepatitis. The genomes of six field EqPV-H strains were sequenced and analyzed, with the results indicating that the Chinese EqPV-H strains have low genetic diversity and high genetic similarity with the USA EqPV-H strain BCT-01. A phylogenetic analysis demonstrated that the Chinese EqPV-H strains clustered with BCT-01 in the genus Copiparvovirus but were distantly related to another equine parvovirus identified in horse cerebrospinal fluid. In addition, liver enzyme levels were detected in the EqPV-H positive serum samples, and all the values were in the normal range, indicating that infection can occur without concurrent liver disease. This study will promote an understanding of the geographical distribution, genetic diversity, and pathogenicity of EqPV-H.http://link.springer.com/article/10.1038/s41426-018-0174-2 |
| spellingShingle | Gang Lu Lingshuang Sun Jiajun Ou Haibin Xu Liyan Wu Shoujun Li Identification and genetic characterization of a novel parvovirus associated with serum hepatitis in horses in China Emerging Microbes and Infections |
| title | Identification and genetic characterization of a novel parvovirus associated with serum hepatitis in horses in China |
| title_full | Identification and genetic characterization of a novel parvovirus associated with serum hepatitis in horses in China |
| title_fullStr | Identification and genetic characterization of a novel parvovirus associated with serum hepatitis in horses in China |
| title_full_unstemmed | Identification and genetic characterization of a novel parvovirus associated with serum hepatitis in horses in China |
| title_short | Identification and genetic characterization of a novel parvovirus associated with serum hepatitis in horses in China |
| title_sort | identification and genetic characterization of a novel parvovirus associated with serum hepatitis in horses in china |
| url | http://link.springer.com/article/10.1038/s41426-018-0174-2 |
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