Protective Effects of the Probiotic Bacterium Streptococcus thermophilus on Candida albicans Morphogenesis and a Murine Model of Oral Candidiasis
Background: Oral candidiasis is a frequent form of candidiasis, caused by Candida species, in particular, Candida albicans (C. albicans). The transition of C. albicans from yeast to hyphae allows its attachment to epithelial cells, followed by biofilm formation, invasion, and tissue damage. Hence, w...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Shiraz University of Medical Sciences
2021-05-01
|
| Series: | Iranian Journal of Medical Sciences |
| Subjects: | |
| Online Access: | https://ijms.sums.ac.ir/article_47039_fa301e2dce7e5fb2d36dbd3756e81baf.pdf |
| _version_ | 1818647250779242496 |
|---|---|
| author | Azita Azad Alireza Ranjbaran Zahra Zareshahrabadi Davood Mehrabani Maryam Zahed Zahedani Asana Talebanpour Kamiar Zomorodian |
| author_facet | Azita Azad Alireza Ranjbaran Zahra Zareshahrabadi Davood Mehrabani Maryam Zahed Zahedani Asana Talebanpour Kamiar Zomorodian |
| author_sort | Azita Azad |
| collection | DOAJ |
| description | Background: Oral candidiasis is a frequent form of candidiasis, caused by Candida species, in particular, Candida albicans (C. albicans). The transition of C. albicans from yeast to hyphae allows its attachment to epithelial cells, followed by biofilm formation, invasion, and tissue damage. Hence, we investigated the effect of Streptococcus salivarius subspecies thermophilus (S thermophilus) on the growth as well as biofilm and germ-tube formation of C. albicans both in vitro and in vivo in a murine model.Methods: This experimental study was performed in the Department of Medical Mycology and Parasitology, School of Medicine, in collaboration with the Central Research Laboratory and the Comparative Biomedical Center, Shiraz University of Medical Sciences, Shiraz, Iran (2017 to 2018). The inhibitory activity of S. thermophilus against Candida species growth was evaluated using the broth microdilution method, and the inhibition of C. albicans biofilm formation was measured using the XTT assay. The inhibition of C. albicans germ-tube formation by S. thermophilus was evaluated using the plate assay and fluorescence microscopy. The experimental activity of the probiotic bacterium was assessed by culture and histopathological methods in six groups of five mice, comprising those treated with four concentrations of probiotics, fluconazole, and distilled water. The one-way analysis of variance, followed by a Tukey post hoc test, was used and a P value of less than 0.05 was considered significant.Results: S. thermophilus inhibited Candida species growth at concentrations of 16 to 512 µg/mL. This probiotic inhibited the formation of C. albicans biofilms and germ tubes in a dose-dependent manner. S. thermophilus significantly reduced the colony-forming units in the mice receiving 30 mg/mL of this probiotic treatment compared with the control group (P=0.024). The histopathological analysis showed that Candida colonization was diminished in the mice following the administration of the probiotic. Conclusion: Given the inhibitory activity of S. thermophilus against the growth, transition, and biofilm formation of C. albicans, it could be used in the management of oral candidiasis. |
| first_indexed | 2024-12-17T00:59:33Z |
| format | Article |
| id | doaj.art-d0736fba2c2943d3a884c44bc437fc5b |
| institution | Directory Open Access Journal |
| issn | 0253-0716 1735-3688 |
| language | English |
| last_indexed | 2024-12-17T00:59:33Z |
| publishDate | 2021-05-01 |
| publisher | Shiraz University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Medical Sciences |
| spelling | doaj.art-d0736fba2c2943d3a884c44bc437fc5b2022-12-21T22:09:31ZengShiraz University of Medical SciencesIranian Journal of Medical Sciences0253-07161735-36882021-05-0146320721710.30476/ijms.2020.82080.047039Protective Effects of the Probiotic Bacterium Streptococcus thermophilus on Candida albicans Morphogenesis and a Murine Model of Oral CandidiasisAzita Azad0Alireza Ranjbaran1Zahra Zareshahrabadi2Davood Mehrabani3Maryam Zahed Zahedani4Asana Talebanpour5Kamiar Zomorodian6Oral and Dental Disease Research Center, Department of Oral and Maxillofacial Medicine, School of Dentistry, Shiraz University of Medical Sciences, Shiraz, IranOral and Dental Disease Research Center, Department of Oral and Maxillofacial Medicine, School of Dentistry, Shiraz University of Medical Sciences, Shiraz, IranDepartment of Parasitology and Mycology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, IranStem Cell and Transgenic Technology Research Center, Shiraz University of Medical Sciences, Shiraz, IranOral and Dental Disease Research Center, Department of Oral and Maxillofacial Medicine, School of Dentistry, Shiraz University of Medical Sciences, Shiraz, IranDepartment of Oral and Maxillofacial Medicine, School of Dentistry, Shiraz University of Medical Sciences, Shiraz, IranDepartment of Parasitology and Mycology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, IranBackground: Oral candidiasis is a frequent form of candidiasis, caused by Candida species, in particular, Candida albicans (C. albicans). The transition of C. albicans from yeast to hyphae allows its attachment to epithelial cells, followed by biofilm formation, invasion, and tissue damage. Hence, we investigated the effect of Streptococcus salivarius subspecies thermophilus (S thermophilus) on the growth as well as biofilm and germ-tube formation of C. albicans both in vitro and in vivo in a murine model.Methods: This experimental study was performed in the Department of Medical Mycology and Parasitology, School of Medicine, in collaboration with the Central Research Laboratory and the Comparative Biomedical Center, Shiraz University of Medical Sciences, Shiraz, Iran (2017 to 2018). The inhibitory activity of S. thermophilus against Candida species growth was evaluated using the broth microdilution method, and the inhibition of C. albicans biofilm formation was measured using the XTT assay. The inhibition of C. albicans germ-tube formation by S. thermophilus was evaluated using the plate assay and fluorescence microscopy. The experimental activity of the probiotic bacterium was assessed by culture and histopathological methods in six groups of five mice, comprising those treated with four concentrations of probiotics, fluconazole, and distilled water. The one-way analysis of variance, followed by a Tukey post hoc test, was used and a P value of less than 0.05 was considered significant.Results: S. thermophilus inhibited Candida species growth at concentrations of 16 to 512 µg/mL. This probiotic inhibited the formation of C. albicans biofilms and germ tubes in a dose-dependent manner. S. thermophilus significantly reduced the colony-forming units in the mice receiving 30 mg/mL of this probiotic treatment compared with the control group (P=0.024). The histopathological analysis showed that Candida colonization was diminished in the mice following the administration of the probiotic. Conclusion: Given the inhibitory activity of S. thermophilus against the growth, transition, and biofilm formation of C. albicans, it could be used in the management of oral candidiasis.https://ijms.sums.ac.ir/article_47039_fa301e2dce7e5fb2d36dbd3756e81baf.pdfcandidastreptococcus salivariusprobioticsbiofilms |
| spellingShingle | Azita Azad Alireza Ranjbaran Zahra Zareshahrabadi Davood Mehrabani Maryam Zahed Zahedani Asana Talebanpour Kamiar Zomorodian Protective Effects of the Probiotic Bacterium Streptococcus thermophilus on Candida albicans Morphogenesis and a Murine Model of Oral Candidiasis Iranian Journal of Medical Sciences candida streptococcus salivarius probiotics biofilms |
| title | Protective Effects of the Probiotic Bacterium Streptococcus thermophilus on Candida albicans Morphogenesis and a Murine Model of Oral Candidiasis |
| title_full | Protective Effects of the Probiotic Bacterium Streptococcus thermophilus on Candida albicans Morphogenesis and a Murine Model of Oral Candidiasis |
| title_fullStr | Protective Effects of the Probiotic Bacterium Streptococcus thermophilus on Candida albicans Morphogenesis and a Murine Model of Oral Candidiasis |
| title_full_unstemmed | Protective Effects of the Probiotic Bacterium Streptococcus thermophilus on Candida albicans Morphogenesis and a Murine Model of Oral Candidiasis |
| title_short | Protective Effects of the Probiotic Bacterium Streptococcus thermophilus on Candida albicans Morphogenesis and a Murine Model of Oral Candidiasis |
| title_sort | protective effects of the probiotic bacterium streptococcus thermophilus on candida albicans morphogenesis and a murine model of oral candidiasis |
| topic | candida streptococcus salivarius probiotics biofilms |
| url | https://ijms.sums.ac.ir/article_47039_fa301e2dce7e5fb2d36dbd3756e81baf.pdf |
| work_keys_str_mv | AT azitaazad protectiveeffectsoftheprobioticbacteriumstreptococcusthermophilusoncandidaalbicansmorphogenesisandamurinemodeloforalcandidiasis AT alirezaranjbaran protectiveeffectsoftheprobioticbacteriumstreptococcusthermophilusoncandidaalbicansmorphogenesisandamurinemodeloforalcandidiasis AT zahrazareshahrabadi protectiveeffectsoftheprobioticbacteriumstreptococcusthermophilusoncandidaalbicansmorphogenesisandamurinemodeloforalcandidiasis AT davoodmehrabani protectiveeffectsoftheprobioticbacteriumstreptococcusthermophilusoncandidaalbicansmorphogenesisandamurinemodeloforalcandidiasis AT maryamzahedzahedani protectiveeffectsoftheprobioticbacteriumstreptococcusthermophilusoncandidaalbicansmorphogenesisandamurinemodeloforalcandidiasis AT asanatalebanpour protectiveeffectsoftheprobioticbacteriumstreptococcusthermophilusoncandidaalbicansmorphogenesisandamurinemodeloforalcandidiasis AT kamiarzomorodian protectiveeffectsoftheprobioticbacteriumstreptococcusthermophilusoncandidaalbicansmorphogenesisandamurinemodeloforalcandidiasis |