<i>MeNINV1</i>: An Alkaline/Neutral Invertase Gene of <i>Manihot esculenta</i>, Enhanced Sucrose Catabolism and Promoted Plant Vegetative Growth in Transgenic <i>Arabidopsis</i>
Alkaline/neutral invertase (A/N-INV) is an invertase that irreversibly decomposes sucrose into fructose as well as glucose and plays a role in plant growth and development, starch synthesis, abiotic stress, and other plant-life activities. Cassava is an economically important starch crop in tropical...
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MDPI AG
2022-03-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/11/7/946 |
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| author | Ya-Jie Wang Xing-Hou Zhen Yang-Jiao Zhou Yun-Lin Wang Jing-Yi Hou Xin Wang Rui-Mei Li Jiao Liu Xin-Wen Hu Meng-Ting Geng Yuan Yao Jian-Chun Guo |
| author_facet | Ya-Jie Wang Xing-Hou Zhen Yang-Jiao Zhou Yun-Lin Wang Jing-Yi Hou Xin Wang Rui-Mei Li Jiao Liu Xin-Wen Hu Meng-Ting Geng Yuan Yao Jian-Chun Guo |
| author_sort | Ya-Jie Wang |
| collection | DOAJ |
| description | Alkaline/neutral invertase (A/N-INV) is an invertase that irreversibly decomposes sucrose into fructose as well as glucose and plays a role in plant growth and development, starch synthesis, abiotic stress, and other plant-life activities. Cassava is an economically important starch crop in tropical regions. During the development of cassava tuber roots, A/N-INV activity is relatively high, which indicates that it may participate in sucrose metabolism and starch synthesis. In this study, MeNINV1 was confirmed to function as invertase to catalyze sucrose decomposition in yeast. The optimal enzymatic properties of MeNINV1 were a pH of 6.5, a reaction temperature of 40 °C, and sucrose as its specific catalytic substrate. VB<sub>6</sub>, Zn<sup>2+</sup>, and Pb<sup>2+</sup> at low concentrations as well as EDTA, DTT, Tris, Mg<sup>2+</sup>, and fructose inhibited A/N-INV enzymic activity. In cassava, the <i>MeNINV1</i> gene was mainly expressed in the fibrous roots and the tuber root phloem, and its expression decreased as the tuber root grew. MeNINV1 was confirmed to localize in chloroplasts. In <i>Arabidopsis</i>, <i>MeNINV1</i>-overexpressing <i>Arabidopsis</i> had higher A/N-INV activity, and the increased glucose, fructose, and starch content in the leaves promoted plant growth and delayed flowering time but did not change its resistance to abiotic stress. Our results provide new insights into the biological function of MeNINV1. |
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| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-09T11:32:02Z |
| publishDate | 2022-03-01 |
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| record_format | Article |
| series | Plants |
| spelling | doaj.art-d074178d2b744d5693b35c2ec3d17b132023-11-30T23:51:15ZengMDPI AGPlants2223-77472022-03-0111794610.3390/plants11070946<i>MeNINV1</i>: An Alkaline/Neutral Invertase Gene of <i>Manihot esculenta</i>, Enhanced Sucrose Catabolism and Promoted Plant Vegetative Growth in Transgenic <i>Arabidopsis</i>Ya-Jie Wang0Xing-Hou Zhen1Yang-Jiao Zhou2Yun-Lin Wang3Jing-Yi Hou4Xin Wang5Rui-Mei Li6Jiao Liu7Xin-Wen Hu8Meng-Ting Geng9Yuan Yao10Jian-Chun Guo11School of Life Sciences, Hainan University, Haikou 570228, ChinaSchool of Life Sciences, Hainan University, Haikou 570228, ChinaSchool of Life Sciences, Hainan University, Haikou 570228, ChinaKey Laboratory of Biology and Genetic Resources of Tropical Crops, Ministry of Agriculture, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, ChinaSchool of Life Sciences, Hainan University, Haikou 570228, ChinaSchool of Life Sciences, Hainan University, Haikou 570228, ChinaKey Laboratory of Biology and Genetic Resources of Tropical Crops, Ministry of Agriculture, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, ChinaKey Laboratory of Biology and Genetic Resources of Tropical Crops, Ministry of Agriculture, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, ChinaSchool of Life Sciences, Hainan University, Haikou 570228, ChinaSchool of Life Sciences, Hainan University, Haikou 570228, ChinaKey Laboratory of Biology and Genetic Resources of Tropical Crops, Ministry of Agriculture, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, ChinaKey Laboratory of Biology and Genetic Resources of Tropical Crops, Ministry of Agriculture, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, ChinaAlkaline/neutral invertase (A/N-INV) is an invertase that irreversibly decomposes sucrose into fructose as well as glucose and plays a role in plant growth and development, starch synthesis, abiotic stress, and other plant-life activities. Cassava is an economically important starch crop in tropical regions. During the development of cassava tuber roots, A/N-INV activity is relatively high, which indicates that it may participate in sucrose metabolism and starch synthesis. In this study, MeNINV1 was confirmed to function as invertase to catalyze sucrose decomposition in yeast. The optimal enzymatic properties of MeNINV1 were a pH of 6.5, a reaction temperature of 40 °C, and sucrose as its specific catalytic substrate. VB<sub>6</sub>, Zn<sup>2+</sup>, and Pb<sup>2+</sup> at low concentrations as well as EDTA, DTT, Tris, Mg<sup>2+</sup>, and fructose inhibited A/N-INV enzymic activity. In cassava, the <i>MeNINV1</i> gene was mainly expressed in the fibrous roots and the tuber root phloem, and its expression decreased as the tuber root grew. MeNINV1 was confirmed to localize in chloroplasts. In <i>Arabidopsis</i>, <i>MeNINV1</i>-overexpressing <i>Arabidopsis</i> had higher A/N-INV activity, and the increased glucose, fructose, and starch content in the leaves promoted plant growth and delayed flowering time but did not change its resistance to abiotic stress. Our results provide new insights into the biological function of MeNINV1.https://www.mdpi.com/2223-7747/11/7/946cassavaalkaline/neutral invertaseMeNINV1<i>Arabidopsis thaliana</i>sucrose metabolismvegetative growth |
| spellingShingle | Ya-Jie Wang Xing-Hou Zhen Yang-Jiao Zhou Yun-Lin Wang Jing-Yi Hou Xin Wang Rui-Mei Li Jiao Liu Xin-Wen Hu Meng-Ting Geng Yuan Yao Jian-Chun Guo <i>MeNINV1</i>: An Alkaline/Neutral Invertase Gene of <i>Manihot esculenta</i>, Enhanced Sucrose Catabolism and Promoted Plant Vegetative Growth in Transgenic <i>Arabidopsis</i> Plants cassava alkaline/neutral invertase MeNINV1 <i>Arabidopsis thaliana</i> sucrose metabolism vegetative growth |
| title | <i>MeNINV1</i>: An Alkaline/Neutral Invertase Gene of <i>Manihot esculenta</i>, Enhanced Sucrose Catabolism and Promoted Plant Vegetative Growth in Transgenic <i>Arabidopsis</i> |
| title_full | <i>MeNINV1</i>: An Alkaline/Neutral Invertase Gene of <i>Manihot esculenta</i>, Enhanced Sucrose Catabolism and Promoted Plant Vegetative Growth in Transgenic <i>Arabidopsis</i> |
| title_fullStr | <i>MeNINV1</i>: An Alkaline/Neutral Invertase Gene of <i>Manihot esculenta</i>, Enhanced Sucrose Catabolism and Promoted Plant Vegetative Growth in Transgenic <i>Arabidopsis</i> |
| title_full_unstemmed | <i>MeNINV1</i>: An Alkaline/Neutral Invertase Gene of <i>Manihot esculenta</i>, Enhanced Sucrose Catabolism and Promoted Plant Vegetative Growth in Transgenic <i>Arabidopsis</i> |
| title_short | <i>MeNINV1</i>: An Alkaline/Neutral Invertase Gene of <i>Manihot esculenta</i>, Enhanced Sucrose Catabolism and Promoted Plant Vegetative Growth in Transgenic <i>Arabidopsis</i> |
| title_sort | i meninv1 i an alkaline neutral invertase gene of i manihot esculenta i enhanced sucrose catabolism and promoted plant vegetative growth in transgenic i arabidopsis i |
| topic | cassava alkaline/neutral invertase MeNINV1 <i>Arabidopsis thaliana</i> sucrose metabolism vegetative growth |
| url | https://www.mdpi.com/2223-7747/11/7/946 |
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