Anoxia Rapidly Induces Changes in Expression of a Large and Diverse Set of Genes in Endothelial Cells
Sinusoidal endothelial cells are the predominant vascular surface of the bone marrow and constitute the functional hematopoietic niche where hematopoietic stem and progenitor cells receive cues for self-renewal, survival, and differentiation. In the bone marrow hematopoietic niche, the oxygen tensio...
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MDPI AG
2023-03-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/6/5157 |
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| author | Antonella Antonelli Emanuele Salvatore Scarpa Santina Bruzzone Cecilia Astigiano Francesco Piacente Michela Bruschi Alessandra Fraternale Christian A. Di Buduo Alessandra Balduini Mauro Magnani |
| author_facet | Antonella Antonelli Emanuele Salvatore Scarpa Santina Bruzzone Cecilia Astigiano Francesco Piacente Michela Bruschi Alessandra Fraternale Christian A. Di Buduo Alessandra Balduini Mauro Magnani |
| author_sort | Antonella Antonelli |
| collection | DOAJ |
| description | Sinusoidal endothelial cells are the predominant vascular surface of the bone marrow and constitute the functional hematopoietic niche where hematopoietic stem and progenitor cells receive cues for self-renewal, survival, and differentiation. In the bone marrow hematopoietic niche, the oxygen tension is usually very low, and this condition affects stem and progenitor cell proliferation and differentiation and other important functions of this region. Here, we have investigated in vitro the response of endothelial cells to a marked decrease in O<sub>2</sub> partial pressure to understand how the basal gene expression of some relevant biological factors (i.e., chemokines and interleukins) that are fundamental for the intercellular communication could change in anoxic conditions. Interestingly, mRNA levels of <i>CXCL3</i>, <i>CXCL5</i>, and <i>IL-34</i> genes are upregulated after anoxia exposure but become downmodulated by sirtuin 6 (SIRT6) overexpression. Indeed, the expression levels of some other genes (such as <i>Leukemia Inhibitory Factor</i> (<i>LIF</i>)) that were not significantly affected by 8 h anoxia exposure become upregulated in the presence of SIRT6. Therefore, SIRT6 mediates also the endothelial cellular response through the modulation of selected genes in an extreme hypoxic condition. |
| first_indexed | 2024-03-11T06:27:29Z |
| format | Article |
| id | doaj.art-d0755413f10849259974c74ef0d75460 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-11T06:27:29Z |
| publishDate | 2023-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-d0755413f10849259974c74ef0d754602023-11-17T11:29:54ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-03-01246515710.3390/ijms24065157Anoxia Rapidly Induces Changes in Expression of a Large and Diverse Set of Genes in Endothelial CellsAntonella Antonelli0Emanuele Salvatore Scarpa1Santina Bruzzone2Cecilia Astigiano3Francesco Piacente4Michela Bruschi5Alessandra Fraternale6Christian A. Di Buduo7Alessandra Balduini8Mauro Magnani9Department of Biomolecular Sciences, University of Urbino Carlo Bo, 61029 Urbino, ItalyDepartment of Biomolecular Sciences, University of Urbino Carlo Bo, 61029 Urbino, ItalyDepartment of Experimental Medicine, Section of Biochemistry, Viale Benedetto XV 1, 16132 Genova, ItalyDepartment of Experimental Medicine, Section of Biochemistry, Viale Benedetto XV 1, 16132 Genova, ItalyDepartment of Experimental Medicine, Section of Biochemistry, Viale Benedetto XV 1, 16132 Genova, ItalyDepartment of Biomolecular Sciences, University of Urbino Carlo Bo, 61029 Urbino, ItalyDepartment of Biomolecular Sciences, University of Urbino Carlo Bo, 61029 Urbino, ItalyDepartment of Molecular Medicine, University of Pavia, 27100 Pavia, ItalyDepartment of Molecular Medicine, University of Pavia, 27100 Pavia, ItalyDepartment of Biomolecular Sciences, University of Urbino Carlo Bo, 61029 Urbino, ItalySinusoidal endothelial cells are the predominant vascular surface of the bone marrow and constitute the functional hematopoietic niche where hematopoietic stem and progenitor cells receive cues for self-renewal, survival, and differentiation. In the bone marrow hematopoietic niche, the oxygen tension is usually very low, and this condition affects stem and progenitor cell proliferation and differentiation and other important functions of this region. Here, we have investigated in vitro the response of endothelial cells to a marked decrease in O<sub>2</sub> partial pressure to understand how the basal gene expression of some relevant biological factors (i.e., chemokines and interleukins) that are fundamental for the intercellular communication could change in anoxic conditions. Interestingly, mRNA levels of <i>CXCL3</i>, <i>CXCL5</i>, and <i>IL-34</i> genes are upregulated after anoxia exposure but become downmodulated by sirtuin 6 (SIRT6) overexpression. Indeed, the expression levels of some other genes (such as <i>Leukemia Inhibitory Factor</i> (<i>LIF</i>)) that were not significantly affected by 8 h anoxia exposure become upregulated in the presence of SIRT6. Therefore, SIRT6 mediates also the endothelial cellular response through the modulation of selected genes in an extreme hypoxic condition.https://www.mdpi.com/1422-0067/24/6/5157anoxiaendothelial cellsinterleukin/cytokine expressiontranscriptome analysisSIRT6 |
| spellingShingle | Antonella Antonelli Emanuele Salvatore Scarpa Santina Bruzzone Cecilia Astigiano Francesco Piacente Michela Bruschi Alessandra Fraternale Christian A. Di Buduo Alessandra Balduini Mauro Magnani Anoxia Rapidly Induces Changes in Expression of a Large and Diverse Set of Genes in Endothelial Cells International Journal of Molecular Sciences anoxia endothelial cells interleukin/cytokine expression transcriptome analysis SIRT6 |
| title | Anoxia Rapidly Induces Changes in Expression of a Large and Diverse Set of Genes in Endothelial Cells |
| title_full | Anoxia Rapidly Induces Changes in Expression of a Large and Diverse Set of Genes in Endothelial Cells |
| title_fullStr | Anoxia Rapidly Induces Changes in Expression of a Large and Diverse Set of Genes in Endothelial Cells |
| title_full_unstemmed | Anoxia Rapidly Induces Changes in Expression of a Large and Diverse Set of Genes in Endothelial Cells |
| title_short | Anoxia Rapidly Induces Changes in Expression of a Large and Diverse Set of Genes in Endothelial Cells |
| title_sort | anoxia rapidly induces changes in expression of a large and diverse set of genes in endothelial cells |
| topic | anoxia endothelial cells interleukin/cytokine expression transcriptome analysis SIRT6 |
| url | https://www.mdpi.com/1422-0067/24/6/5157 |
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