Viability of unstimulated lymphocytes exposed to extremely low frequency electromagnetic fields is dependent on intensity
The cell viability and DNA damage in unstimulated sheep primary lymphocytes subjected to different extremely low electromagnetic field intensities (5, 50 and 100 µT; 50 Hz) were studied with special emphasis on apoptosis. Sheep primary lymphocytes cultured in RPMI, supplemented with 10% FBS in the a...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Pensoft Publishers
2012-08-01
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| Series: | BioDiscovery |
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| Online Access: | https://biodiscovery.pensoft.net/article/8925/download/pdf/ |
| _version_ | 1828184149616230400 |
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| author | P Rajendra HN Sujatha RB Sashidhar C Subramanyam D Devendranath RSS Aradhya |
| author_facet | P Rajendra HN Sujatha RB Sashidhar C Subramanyam D Devendranath RSS Aradhya |
| author_sort | P Rajendra |
| collection | DOAJ |
| description | The cell viability and DNA damage in unstimulated sheep primary lymphocytes subjected to different extremely low electromagnetic field intensities (5, 50 and 100 µT; 50 Hz) were studied with special emphasis on apoptosis. Sheep primary lymphocytes cultured in RPMI, supplemented with 10% FBS in the absence of mitogens, were exposed till 16 h. The cell viability assessment by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide (MTT) assay showed a dose dependent enhancement of viability at 16 h. Further, quantitative DNA laddering and flow cytometric analysis showed a significant decrease in apoptosis of the cells subjected to 100 (p |
| first_indexed | 2024-04-12T06:44:07Z |
| format | Article |
| id | doaj.art-d07675e10f3442cd93c83bf02a46ccc2 |
| institution | Directory Open Access Journal |
| issn | 2050-2966 |
| language | English |
| last_indexed | 2024-04-12T06:44:07Z |
| publishDate | 2012-08-01 |
| publisher | Pensoft Publishers |
| record_format | Article |
| series | BioDiscovery |
| spelling | doaj.art-d07675e10f3442cd93c83bf02a46ccc22022-12-22T03:43:38ZengPensoft PublishersBioDiscovery2050-29662012-08-01211110.7750/BioDiscovery.2012.2.28925Viability of unstimulated lymphocytes exposed to extremely low frequency electromagnetic fields is dependent on intensityP RajendraHN SujathaRB SashidharC SubramanyamD DevendranathRSS AradhyaThe cell viability and DNA damage in unstimulated sheep primary lymphocytes subjected to different extremely low electromagnetic field intensities (5, 50 and 100 µT; 50 Hz) were studied with special emphasis on apoptosis. Sheep primary lymphocytes cultured in RPMI, supplemented with 10% FBS in the absence of mitogens, were exposed till 16 h. The cell viability assessment by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide (MTT) assay showed a dose dependent enhancement of viability at 16 h. Further, quantitative DNA laddering and flow cytometric analysis showed a significant decrease in apoptosis of the cells subjected to 100 (phttps://biodiscovery.pensoft.net/article/8925/download/pdf/ELF-EMFprimary lymphocytesapoptosisDNA damag |
| spellingShingle | P Rajendra HN Sujatha RB Sashidhar C Subramanyam D Devendranath RSS Aradhya Viability of unstimulated lymphocytes exposed to extremely low frequency electromagnetic fields is dependent on intensity BioDiscovery ELF-EMF primary lymphocytes apoptosis DNA damag |
| title | Viability of unstimulated lymphocytes exposed to extremely low frequency electromagnetic fields is dependent on intensity |
| title_full | Viability of unstimulated lymphocytes exposed to extremely low frequency electromagnetic fields is dependent on intensity |
| title_fullStr | Viability of unstimulated lymphocytes exposed to extremely low frequency electromagnetic fields is dependent on intensity |
| title_full_unstemmed | Viability of unstimulated lymphocytes exposed to extremely low frequency electromagnetic fields is dependent on intensity |
| title_short | Viability of unstimulated lymphocytes exposed to extremely low frequency electromagnetic fields is dependent on intensity |
| title_sort | viability of unstimulated lymphocytes exposed to extremely low frequency electromagnetic fields is dependent on intensity |
| topic | ELF-EMF primary lymphocytes apoptosis DNA damag |
| url | https://biodiscovery.pensoft.net/article/8925/download/pdf/ |
| work_keys_str_mv | AT prajendra viabilityofunstimulatedlymphocytesexposedtoextremelylowfrequencyelectromagneticfieldsisdependentonintensity AT hnsujatha viabilityofunstimulatedlymphocytesexposedtoextremelylowfrequencyelectromagneticfieldsisdependentonintensity AT rbsashidhar viabilityofunstimulatedlymphocytesexposedtoextremelylowfrequencyelectromagneticfieldsisdependentonintensity AT csubramanyam viabilityofunstimulatedlymphocytesexposedtoextremelylowfrequencyelectromagneticfieldsisdependentonintensity AT ddevendranath viabilityofunstimulatedlymphocytesexposedtoextremelylowfrequencyelectromagneticfieldsisdependentonintensity AT rssaradhya viabilityofunstimulatedlymphocytesexposedtoextremelylowfrequencyelectromagneticfieldsisdependentonintensity |