Phylogeography and Biological Characterizations of H12 Influenza A Viruses
Influenza A virus (IAV) is widespread in wild bird reservoirs. Sixteen hemagglutinin subtypes are associated with wild waterfowl hosts; some subtypes are isolated infrequently, one of which is H12 IAV. In this study, we detected three H12 IAVs from <i>Anas</i><i>crecca</i> an...
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MDPI AG
2022-10-01
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| author | Zhimin Wan Qiuqi Kan Dongchang He Zhehong Zhao Jianxi Gong Wenjie Jiang Ting Tang Yafeng Li Quan Xie Tuofan Li Hongxia Shao Aijian Qin Jianqiang Ye |
| author_facet | Zhimin Wan Qiuqi Kan Dongchang He Zhehong Zhao Jianxi Gong Wenjie Jiang Ting Tang Yafeng Li Quan Xie Tuofan Li Hongxia Shao Aijian Qin Jianqiang Ye |
| author_sort | Zhimin Wan |
| collection | DOAJ |
| description | Influenza A virus (IAV) is widespread in wild bird reservoirs. Sixteen hemagglutinin subtypes are associated with wild waterfowl hosts; some subtypes are isolated infrequently, one of which is H12 IAV. In this study, we detected three H12 IAVs from <i>Anas</i><i>crecca</i> and <i>Anas formosa</i> in Poyang Lake, China, in 2018, one of which was isolated. Phylogenetic analysis revealed that the genome sequences of the three H12 viruses belonged to the Eurasian lineage, except for PA genes and one NP gene, which belonged to the North American lineage. The growth kinetics showed that the H12 isolate grew better in A549 than MDCK cells. Moreover, although the H12 isolate cannot efficiently replicate in BALB/c mice, it can bind to both <i>α</i>-2,6 sialic acid (SA) and <i>α</i>-2,SA-linked receptors. In addition, we examined the phylodynamics of H12 viruses by Bayesian phylogeographic analysis. The results show that two major transmission routes of H12 IAVs were from Asia to Oceania and from Europe to South America, and <i>Anas</i> and <i>Arenaria</i> genera were the major hosts of the viral transmission. Our findings help us better understand the evolution of H12 IAV and highlight the need for the continued surveillance of IAVs circulating in wild birds. |
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| language | English |
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| series | Viruses |
| spelling | doaj.art-d079b9c41c7e4dbba62aa90285cd8b8e2023-11-24T03:10:10ZengMDPI AGViruses1999-49152022-10-011410225110.3390/v14102251Phylogeography and Biological Characterizations of H12 Influenza A VirusesZhimin Wan0Qiuqi Kan1Dongchang He2Zhehong Zhao3Jianxi Gong4Wenjie Jiang5Ting Tang6Yafeng Li7Quan Xie8Tuofan Li9Hongxia Shao10Aijian Qin11Jianqiang Ye12Key Laboratory of Jiangsu Preventive Veterinary Medicine, Key Laboratory for Avian Preventive Medicine, Ministry of Education, College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, ChinaKey Laboratory of Jiangsu Preventive Veterinary Medicine, Key Laboratory for Avian Preventive Medicine, Ministry of Education, College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, ChinaKey Laboratory of Jiangsu Preventive Veterinary Medicine, Key Laboratory for Avian Preventive Medicine, Ministry of Education, College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, ChinaKey Laboratory of Jiangsu Preventive Veterinary Medicine, Key Laboratory for Avian Preventive Medicine, Ministry of Education, College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, ChinaKey Laboratory of Jiangsu Preventive Veterinary Medicine, Key Laboratory for Avian Preventive Medicine, Ministry of Education, College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, ChinaKey Laboratory of Jiangsu Preventive Veterinary Medicine, Key Laboratory for Avian Preventive Medicine, Ministry of Education, College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, ChinaKey Laboratory of Jiangsu Preventive Veterinary Medicine, Key Laboratory for Avian Preventive Medicine, Ministry of Education, College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, ChinaKey Laboratory of Jiangsu Preventive Veterinary Medicine, Key Laboratory for Avian Preventive Medicine, Ministry of Education, College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, ChinaKey Laboratory of Jiangsu Preventive Veterinary Medicine, Key Laboratory for Avian Preventive Medicine, Ministry of Education, College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, ChinaKey Laboratory of Jiangsu Preventive Veterinary Medicine, Key Laboratory for Avian Preventive Medicine, Ministry of Education, College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, ChinaKey Laboratory of Jiangsu Preventive Veterinary Medicine, Key Laboratory for Avian Preventive Medicine, Ministry of Education, College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, ChinaKey Laboratory of Jiangsu Preventive Veterinary Medicine, Key Laboratory for Avian Preventive Medicine, Ministry of Education, College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, ChinaKey Laboratory of Jiangsu Preventive Veterinary Medicine, Key Laboratory for Avian Preventive Medicine, Ministry of Education, College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, ChinaInfluenza A virus (IAV) is widespread in wild bird reservoirs. Sixteen hemagglutinin subtypes are associated with wild waterfowl hosts; some subtypes are isolated infrequently, one of which is H12 IAV. In this study, we detected three H12 IAVs from <i>Anas</i><i>crecca</i> and <i>Anas formosa</i> in Poyang Lake, China, in 2018, one of which was isolated. Phylogenetic analysis revealed that the genome sequences of the three H12 viruses belonged to the Eurasian lineage, except for PA genes and one NP gene, which belonged to the North American lineage. The growth kinetics showed that the H12 isolate grew better in A549 than MDCK cells. Moreover, although the H12 isolate cannot efficiently replicate in BALB/c mice, it can bind to both <i>α</i>-2,6 sialic acid (SA) and <i>α</i>-2,SA-linked receptors. In addition, we examined the phylodynamics of H12 viruses by Bayesian phylogeographic analysis. The results show that two major transmission routes of H12 IAVs were from Asia to Oceania and from Europe to South America, and <i>Anas</i> and <i>Arenaria</i> genera were the major hosts of the viral transmission. Our findings help us better understand the evolution of H12 IAV and highlight the need for the continued surveillance of IAVs circulating in wild birds.https://www.mdpi.com/1999-4915/14/10/2251H12 subtype IAVwild birdsevolutionbiological characterizationsphylodynamics |
| spellingShingle | Zhimin Wan Qiuqi Kan Dongchang He Zhehong Zhao Jianxi Gong Wenjie Jiang Ting Tang Yafeng Li Quan Xie Tuofan Li Hongxia Shao Aijian Qin Jianqiang Ye Phylogeography and Biological Characterizations of H12 Influenza A Viruses Viruses H12 subtype IAV wild birds evolution biological characterizations phylodynamics |
| title | Phylogeography and Biological Characterizations of H12 Influenza A Viruses |
| title_full | Phylogeography and Biological Characterizations of H12 Influenza A Viruses |
| title_fullStr | Phylogeography and Biological Characterizations of H12 Influenza A Viruses |
| title_full_unstemmed | Phylogeography and Biological Characterizations of H12 Influenza A Viruses |
| title_short | Phylogeography and Biological Characterizations of H12 Influenza A Viruses |
| title_sort | phylogeography and biological characterizations of h12 influenza a viruses |
| topic | H12 subtype IAV wild birds evolution biological characterizations phylodynamics |
| url | https://www.mdpi.com/1999-4915/14/10/2251 |
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