Phylogeography of cliff racer (Platyceps rhodorachis Jan, 1865) from Punjab, Pakistan
Abstract The present study reports the existence of cliff racer, Platyceps rhodorachis from the plains of Punjab, Pakistan. A total of 10 specimens were captured during the field surveys from June to September, 2018 from different sites of Punjab. Platyceps rhodorachis was identify on the basis of m...
| Main Authors: | , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Instituto Internacional de Ecologia
2021-08-01
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| Series: | Brazilian Journal of Biology |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-69842023000100182&tlng=en |
| _version_ | 1798019848750497792 |
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| author | S. Malik A. Javid Hamidullah M. A. Iqbal A. Hussain S. M. Bukhari A. L. Chaber F. Hemmatzadeh N. Ismat A. Ali S. M. Azam G. Mustafa S. Hussain U. Ahmad A. Ahmad W. Ali |
| author_facet | S. Malik A. Javid Hamidullah M. A. Iqbal A. Hussain S. M. Bukhari A. L. Chaber F. Hemmatzadeh N. Ismat A. Ali S. M. Azam G. Mustafa S. Hussain U. Ahmad A. Ahmad W. Ali |
| author_sort | S. Malik |
| collection | DOAJ |
| description | Abstract The present study reports the existence of cliff racer, Platyceps rhodorachis from the plains of Punjab, Pakistan. A total of 10 specimens were captured during the field surveys from June to September, 2018 from different sites of Punjab. Platyceps rhodorachis was identify on the basis of morphology and confirmed through COI gene sequences. The obtained DNA sequences have shown reliable and exact species identification. Newly produced DNA sequences of Platyceps rhodorachis were submitted to GenBank and accession numbers were obtained (MK936174.1, MK941839.1 and MT790210.1). N-J tree based on COI sequences of Platyceps rhodorachis clearly separated as out-group with other members of family Colubridae based on p-distance. The intra-specific genetic variation ranges from 12% to 18%. The DNA sequences of Platyceps rhodorachis kashmirensis, Platyceps rhodorachis ladacensis, Platyceps ventromaculatus, Platyceps ventromaculatus bengalensis and Platyceps ventromaculatus indusai are not available at NCBI to validate their taxonomic positions. In our recommendations, a large scale molecular based identification of Pakistan’s herpetofauna is required to report more new or subspecies from country. |
| first_indexed | 2024-04-11T16:47:38Z |
| format | Article |
| id | doaj.art-d07ba4672506411f8992a1374e7b2a6a |
| institution | Directory Open Access Journal |
| issn | 1678-4375 |
| language | English |
| last_indexed | 2024-04-11T16:47:38Z |
| publishDate | 2021-08-01 |
| publisher | Instituto Internacional de Ecologia |
| record_format | Article |
| series | Brazilian Journal of Biology |
| spelling | doaj.art-d07ba4672506411f8992a1374e7b2a6a2022-12-22T04:13:32ZengInstituto Internacional de EcologiaBrazilian Journal of Biology1678-43752021-08-018310.1590/1519-6984.246243Phylogeography of cliff racer (Platyceps rhodorachis Jan, 1865) from Punjab, PakistanS. Malikhttps://orcid.org/0000-0002-8762-6289A. Javidhttps://orcid.org/0000-0002-2620-1447 Hamidullahhttps://orcid.org/0000-0003-0953-0158M. A. Iqbalhttps://orcid.org/0000-0001-8069-1320A. Hussainhttps://orcid.org/0000-0002-8105-3029S. M. Bukharihttps://orcid.org/0000-0002-6330-9676A. L. Chaberhttps://orcid.org/0000-0001-9042-8753F. Hemmatzadehhttps://orcid.org/0000-0002-4572-8869N. Ismathttps://orcid.org/0000-0001-7295-1452A. Alihttps://orcid.org/0000-0002-8405-1686S. M. Azamhttps://orcid.org/0000-0001-8966-5698G. Mustafahttps://orcid.org/0000-0003-1921-3316S. Hussainhttps://orcid.org/0000-0001-9042-2810U. Ahmadhttps://orcid.org/0000-0003-1725-515XA. Ahmadhttps://orcid.org/0000-0003-2564-7241W. Alihttps://orcid.org/0000-0002-4074-9898Abstract The present study reports the existence of cliff racer, Platyceps rhodorachis from the plains of Punjab, Pakistan. A total of 10 specimens were captured during the field surveys from June to September, 2018 from different sites of Punjab. Platyceps rhodorachis was identify on the basis of morphology and confirmed through COI gene sequences. The obtained DNA sequences have shown reliable and exact species identification. Newly produced DNA sequences of Platyceps rhodorachis were submitted to GenBank and accession numbers were obtained (MK936174.1, MK941839.1 and MT790210.1). N-J tree based on COI sequences of Platyceps rhodorachis clearly separated as out-group with other members of family Colubridae based on p-distance. The intra-specific genetic variation ranges from 12% to 18%. The DNA sequences of Platyceps rhodorachis kashmirensis, Platyceps rhodorachis ladacensis, Platyceps ventromaculatus, Platyceps ventromaculatus bengalensis and Platyceps ventromaculatus indusai are not available at NCBI to validate their taxonomic positions. In our recommendations, a large scale molecular based identification of Pakistan’s herpetofauna is required to report more new or subspecies from country.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-69842023000100182&tlng=enDNA barcodingCOIPlatyceps rhodorachis kashmirensisPlatyceps ventromaculatus bengalensisPtyas mucosus |
| spellingShingle | S. Malik A. Javid Hamidullah M. A. Iqbal A. Hussain S. M. Bukhari A. L. Chaber F. Hemmatzadeh N. Ismat A. Ali S. M. Azam G. Mustafa S. Hussain U. Ahmad A. Ahmad W. Ali Phylogeography of cliff racer (Platyceps rhodorachis Jan, 1865) from Punjab, Pakistan Brazilian Journal of Biology DNA barcoding COI Platyceps rhodorachis kashmirensis Platyceps ventromaculatus bengalensis Ptyas mucosus |
| title | Phylogeography of cliff racer (Platyceps rhodorachis Jan, 1865) from Punjab, Pakistan |
| title_full | Phylogeography of cliff racer (Platyceps rhodorachis Jan, 1865) from Punjab, Pakistan |
| title_fullStr | Phylogeography of cliff racer (Platyceps rhodorachis Jan, 1865) from Punjab, Pakistan |
| title_full_unstemmed | Phylogeography of cliff racer (Platyceps rhodorachis Jan, 1865) from Punjab, Pakistan |
| title_short | Phylogeography of cliff racer (Platyceps rhodorachis Jan, 1865) from Punjab, Pakistan |
| title_sort | phylogeography of cliff racer platyceps rhodorachis jan 1865 from punjab pakistan |
| topic | DNA barcoding COI Platyceps rhodorachis kashmirensis Platyceps ventromaculatus bengalensis Ptyas mucosus |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-69842023000100182&tlng=en |
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