Acute Aerobic Exercise Remodels the Adipose Tissue Progenitor Cell Phenotype in Obese Adults
Adipose tissue pathology in obese patients often features impaired adipogenesis, angiogenesis, and chronic low-grade inflammation, all of which are regulated in large part by adipose tissue stromal vascular cells [SVC; i.e., non-adipocyte cells within adipose tissue including preadipocytes, endothel...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2020-07-01
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| Series: | Frontiers in Physiology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fphys.2020.00903/full |
| _version_ | 1819290254024114176 |
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| author | Alison C. Ludzki Emily M. Krueger Toree C. Baldwin Michael W. Schleh Cara E. Porsche Cara E. Porsche Benjamin J. Ryan Lindsey A. Muir Kanakadurga Singer Kanakadurga Singer Kanakadurga Singer Carey N. Lumeng Carey N. Lumeng Carey N. Lumeng Jeffrey F. Horowitz |
| author_facet | Alison C. Ludzki Emily M. Krueger Toree C. Baldwin Michael W. Schleh Cara E. Porsche Cara E. Porsche Benjamin J. Ryan Lindsey A. Muir Kanakadurga Singer Kanakadurga Singer Kanakadurga Singer Carey N. Lumeng Carey N. Lumeng Carey N. Lumeng Jeffrey F. Horowitz |
| author_sort | Alison C. Ludzki |
| collection | DOAJ |
| description | Adipose tissue pathology in obese patients often features impaired adipogenesis, angiogenesis, and chronic low-grade inflammation, all of which are regulated in large part by adipose tissue stromal vascular cells [SVC; i.e., non-adipocyte cells within adipose tissue including preadipocytes, endothelial cells (ECs), and immune cells]. Exercise is known to increase subcutaneous adipose tissue lipolysis, but the impact of exercise on SVCs in adipose tissue has not been explored. The purpose of this study was to assess the effects of a session of exercise on preadipocyte, EC, macrophage, and T cell content in human subcutaneous adipose tissue. We collected abdominal subcutaneous adipose tissue samples from 10 obese adults (BMI 33 ± 3 kg/m2, body fat 41 ± 7%) 12 h after a 60 min acute session of endurance exercise (80 ± 3%HRpeak) vs. no acute exercise session. SVCs were isolated by collagenase digestion and stained for flow cytometry. We found that acute exercise reduced preadipocyte content (38 ± 7 vs. 30 ± 13%SVC; p = 0.04). The reduction was driven by a decrease in CD34hi preadipocytes (18 ± 5 vs. 13 ± 6%SVC; p = 0.002), a subset of preadipocytes that generates high lipolytic rate adipocytes ex vivo. Acute exercise did not alter EC content. Acute exercise also did not change total immune cell, macrophage, or T cell content, and future work should assess the effects of exercise on subpopulations of these cells. We conclude that exercise may rapidly regulate the subcutaneous adipose tissue preadipocyte pool in ways that may help attenuate the high lipolytic rates that are commonly found in obesity. |
| first_indexed | 2024-12-24T03:19:49Z |
| format | Article |
| id | doaj.art-d07f41fb589b4cd1b8d4d8a8ee71bc61 |
| institution | Directory Open Access Journal |
| issn | 1664-042X |
| language | English |
| last_indexed | 2024-12-24T03:19:49Z |
| publishDate | 2020-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Physiology |
| spelling | doaj.art-d07f41fb589b4cd1b8d4d8a8ee71bc612022-12-21T17:17:32ZengFrontiers Media S.A.Frontiers in Physiology1664-042X2020-07-011110.3389/fphys.2020.00903560263Acute Aerobic Exercise Remodels the Adipose Tissue Progenitor Cell Phenotype in Obese AdultsAlison C. Ludzki0Emily M. Krueger1Toree C. Baldwin2Michael W. Schleh3Cara E. Porsche4Cara E. Porsche5Benjamin J. Ryan6Lindsey A. Muir7Kanakadurga Singer8Kanakadurga Singer9Kanakadurga Singer10Carey N. Lumeng11Carey N. Lumeng12Carey N. Lumeng13Jeffrey F. Horowitz14Substrate Metabolism Laboratory, School of Kinesiology, University of Michigan, Ann Arbor, MI, United StatesSubstrate Metabolism Laboratory, School of Kinesiology, University of Michigan, Ann Arbor, MI, United StatesSubstrate Metabolism Laboratory, School of Kinesiology, University of Michigan, Ann Arbor, MI, United StatesSubstrate Metabolism Laboratory, School of Kinesiology, University of Michigan, Ann Arbor, MI, United StatesDepartment of Pediatrics, Medical School, University of Michigan, Ann Arbor, MI, United StatesGraduate Program in Immunology, Medical School, University of Michigan, Ann Arbor, MI, United StatesSubstrate Metabolism Laboratory, School of Kinesiology, University of Michigan, Ann Arbor, MI, United StatesDepartment of Computational Medicine and Bioinformatics, Medical School, University of Michigan, Ann Arbor, MI, United StatesDepartment of Pediatrics, Medical School, University of Michigan, Ann Arbor, MI, United StatesGraduate Program in Immunology, Medical School, University of Michigan, Ann Arbor, MI, United StatesDepartment of Molecular and Integrative Physiology, Medical School, University of Michigan, Ann Arbor, MI, United StatesDepartment of Pediatrics, Medical School, University of Michigan, Ann Arbor, MI, United StatesGraduate Program in Immunology, Medical School, University of Michigan, Ann Arbor, MI, United StatesDepartment of Molecular and Integrative Physiology, Medical School, University of Michigan, Ann Arbor, MI, United StatesSubstrate Metabolism Laboratory, School of Kinesiology, University of Michigan, Ann Arbor, MI, United StatesAdipose tissue pathology in obese patients often features impaired adipogenesis, angiogenesis, and chronic low-grade inflammation, all of which are regulated in large part by adipose tissue stromal vascular cells [SVC; i.e., non-adipocyte cells within adipose tissue including preadipocytes, endothelial cells (ECs), and immune cells]. Exercise is known to increase subcutaneous adipose tissue lipolysis, but the impact of exercise on SVCs in adipose tissue has not been explored. The purpose of this study was to assess the effects of a session of exercise on preadipocyte, EC, macrophage, and T cell content in human subcutaneous adipose tissue. We collected abdominal subcutaneous adipose tissue samples from 10 obese adults (BMI 33 ± 3 kg/m2, body fat 41 ± 7%) 12 h after a 60 min acute session of endurance exercise (80 ± 3%HRpeak) vs. no acute exercise session. SVCs were isolated by collagenase digestion and stained for flow cytometry. We found that acute exercise reduced preadipocyte content (38 ± 7 vs. 30 ± 13%SVC; p = 0.04). The reduction was driven by a decrease in CD34hi preadipocytes (18 ± 5 vs. 13 ± 6%SVC; p = 0.002), a subset of preadipocytes that generates high lipolytic rate adipocytes ex vivo. Acute exercise did not alter EC content. Acute exercise also did not change total immune cell, macrophage, or T cell content, and future work should assess the effects of exercise on subpopulations of these cells. We conclude that exercise may rapidly regulate the subcutaneous adipose tissue preadipocyte pool in ways that may help attenuate the high lipolytic rates that are commonly found in obesity.https://www.frontiersin.org/article/10.3389/fphys.2020.00903/fullpreadipocytesexerciseobesitymacrophagesendothelial cellsT cells |
| spellingShingle | Alison C. Ludzki Emily M. Krueger Toree C. Baldwin Michael W. Schleh Cara E. Porsche Cara E. Porsche Benjamin J. Ryan Lindsey A. Muir Kanakadurga Singer Kanakadurga Singer Kanakadurga Singer Carey N. Lumeng Carey N. Lumeng Carey N. Lumeng Jeffrey F. Horowitz Acute Aerobic Exercise Remodels the Adipose Tissue Progenitor Cell Phenotype in Obese Adults Frontiers in Physiology preadipocytes exercise obesity macrophages endothelial cells T cells |
| title | Acute Aerobic Exercise Remodels the Adipose Tissue Progenitor Cell Phenotype in Obese Adults |
| title_full | Acute Aerobic Exercise Remodels the Adipose Tissue Progenitor Cell Phenotype in Obese Adults |
| title_fullStr | Acute Aerobic Exercise Remodels the Adipose Tissue Progenitor Cell Phenotype in Obese Adults |
| title_full_unstemmed | Acute Aerobic Exercise Remodels the Adipose Tissue Progenitor Cell Phenotype in Obese Adults |
| title_short | Acute Aerobic Exercise Remodels the Adipose Tissue Progenitor Cell Phenotype in Obese Adults |
| title_sort | acute aerobic exercise remodels the adipose tissue progenitor cell phenotype in obese adults |
| topic | preadipocytes exercise obesity macrophages endothelial cells T cells |
| url | https://www.frontiersin.org/article/10.3389/fphys.2020.00903/full |
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