Integrated into Environmental Biofilm <i>Chromobacterium vaccinii</i> Survives Winter with Support of Bacterial Community
<i>Chromobacterium</i> species are common in tropical and subtropical zones in environmental samples according to numerous studies. Here, we describe an environmental case of resident <i>Chromobacterium vaccinii</i> in biofilms associated with <i>Carex</i> spp. ro...
| Main Authors: | , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-10-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/8/11/1696 |
| _version_ | 1827703150891499520 |
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| author | Daria A. Egorova Olga L. Voronina Andrey I. Solovyev Marina S. Kunda Ekaterina I. Aksenova Natalia N. Ryzhova Ksenya V. Danilova Valentina S. Rykova Anastasya A. Scherbakova Andrey N. Semenov Nikita B. Polyakov Daniil A. Grumov Natalia V. Shevlyagina Inna V. Dolzhikova Yulia M. Romanova Alexander L. Gintsburg |
| author_facet | Daria A. Egorova Olga L. Voronina Andrey I. Solovyev Marina S. Kunda Ekaterina I. Aksenova Natalia N. Ryzhova Ksenya V. Danilova Valentina S. Rykova Anastasya A. Scherbakova Andrey N. Semenov Nikita B. Polyakov Daniil A. Grumov Natalia V. Shevlyagina Inna V. Dolzhikova Yulia M. Romanova Alexander L. Gintsburg |
| author_sort | Daria A. Egorova |
| collection | DOAJ |
| description | <i>Chromobacterium</i> species are common in tropical and subtropical zones in environmental samples according to numerous studies. Here, we describe an environmental case of resident <i>Chromobacterium vaccinii</i> in biofilms associated with <i>Carex</i> spp. roots in Moscow region, Russia (warm-summer humid continental climate zone). We performed broad characterization of individual properties as well as surrounding context for better understanding of the premise of <i>C. vaccinii</i> survival during the winter season. Genome properties of isolated strains propose some insights into adaptation to habit and biofilm mode of life, including social cheaters carrying <i>ΔluxR</i> mutation. Isolated <i>C. vaccinii</i> differs from previously described strains in some biochemical properties and some basic characteristics like fatty acid composition as well as unique genome features. Despite potential to modulate membrane fluidity and presence of several genes responsible for cold shock response, isolated <i>C. vaccinii</i> did not survive during exposure to 4 °C, while in the complex biofilm sample, it was safely preserved for at least half a year in vitro at 4 °C. The surrounding bacterial community within the same biofilm with <i>C. vaccinii</i> represented a series of psychrophilic bacterial species, which may share resistance to low temperatures with other species within biofilm and provide <i>C. vaccinii</i> an opportunity to survive during the cold winter season. |
| first_indexed | 2024-03-10T15:12:21Z |
| format | Article |
| id | doaj.art-d081a2110abf4d1ea797905d61e051ba |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-10T15:12:21Z |
| publishDate | 2020-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-d081a2110abf4d1ea797905d61e051ba2023-11-20T19:14:47ZengMDPI AGMicroorganisms2076-26072020-10-01811169610.3390/microorganisms8111696Integrated into Environmental Biofilm <i>Chromobacterium vaccinii</i> Survives Winter with Support of Bacterial CommunityDaria A. Egorova0Olga L. Voronina1Andrey I. Solovyev2Marina S. Kunda3Ekaterina I. Aksenova4Natalia N. Ryzhova5Ksenya V. Danilova6Valentina S. Rykova7Anastasya A. Scherbakova8Andrey N. Semenov9Nikita B. Polyakov10Daniil A. Grumov11Natalia V. Shevlyagina12Inna V. Dolzhikova13Yulia M. Romanova14Alexander L. Gintsburg15N.F. Gamaleya National Research Center of Epidemiology and Microbiology, Ministry of Health, 123098 Moscow, RussiaN.F. Gamaleya National Research Center of Epidemiology and Microbiology, Ministry of Health, 123098 Moscow, RussiaN.F. Gamaleya National Research Center of Epidemiology and Microbiology, Ministry of Health, 123098 Moscow, RussiaN.F. Gamaleya National Research Center of Epidemiology and Microbiology, Ministry of Health, 123098 Moscow, RussiaN.F. Gamaleya National Research Center of Epidemiology and Microbiology, Ministry of Health, 123098 Moscow, RussiaN.F. Gamaleya National Research Center of Epidemiology and Microbiology, Ministry of Health, 123098 Moscow, RussiaN.F. Gamaleya National Research Center of Epidemiology and Microbiology, Ministry of Health, 123098 Moscow, RussiaN.F. Gamaleya National Research Center of Epidemiology and Microbiology, Ministry of Health, 123098 Moscow, RussiaN.F. Gamaleya National Research Center of Epidemiology and Microbiology, Ministry of Health, 123098 Moscow, RussiaN.F. Gamaleya National Research Center of Epidemiology and Microbiology, Ministry of Health, 123098 Moscow, RussiaN.F. Gamaleya National Research Center of Epidemiology and Microbiology, Ministry of Health, 123098 Moscow, RussiaN.F. Gamaleya National Research Center of Epidemiology and Microbiology, Ministry of Health, 123098 Moscow, RussiaN.F. Gamaleya National Research Center of Epidemiology and Microbiology, Ministry of Health, 123098 Moscow, RussiaN.F. Gamaleya National Research Center of Epidemiology and Microbiology, Ministry of Health, 123098 Moscow, RussiaN.F. Gamaleya National Research Center of Epidemiology and Microbiology, Ministry of Health, 123098 Moscow, RussiaN.F. Gamaleya National Research Center of Epidemiology and Microbiology, Ministry of Health, 123098 Moscow, Russia<i>Chromobacterium</i> species are common in tropical and subtropical zones in environmental samples according to numerous studies. Here, we describe an environmental case of resident <i>Chromobacterium vaccinii</i> in biofilms associated with <i>Carex</i> spp. roots in Moscow region, Russia (warm-summer humid continental climate zone). We performed broad characterization of individual properties as well as surrounding context for better understanding of the premise of <i>C. vaccinii</i> survival during the winter season. Genome properties of isolated strains propose some insights into adaptation to habit and biofilm mode of life, including social cheaters carrying <i>ΔluxR</i> mutation. Isolated <i>C. vaccinii</i> differs from previously described strains in some biochemical properties and some basic characteristics like fatty acid composition as well as unique genome features. Despite potential to modulate membrane fluidity and presence of several genes responsible for cold shock response, isolated <i>C. vaccinii</i> did not survive during exposure to 4 °C, while in the complex biofilm sample, it was safely preserved for at least half a year in vitro at 4 °C. The surrounding bacterial community within the same biofilm with <i>C. vaccinii</i> represented a series of psychrophilic bacterial species, which may share resistance to low temperatures with other species within biofilm and provide <i>C. vaccinii</i> an opportunity to survive during the cold winter season.https://www.mdpi.com/2076-2607/8/11/1696<i>Chromobacterium vaccinii</i>biofilmsharing goodssocial cheaterbog microbiomebacterial genome |
| spellingShingle | Daria A. Egorova Olga L. Voronina Andrey I. Solovyev Marina S. Kunda Ekaterina I. Aksenova Natalia N. Ryzhova Ksenya V. Danilova Valentina S. Rykova Anastasya A. Scherbakova Andrey N. Semenov Nikita B. Polyakov Daniil A. Grumov Natalia V. Shevlyagina Inna V. Dolzhikova Yulia M. Romanova Alexander L. Gintsburg Integrated into Environmental Biofilm <i>Chromobacterium vaccinii</i> Survives Winter with Support of Bacterial Community Microorganisms <i>Chromobacterium vaccinii</i> biofilm sharing goods social cheater bog microbiome bacterial genome |
| title | Integrated into Environmental Biofilm <i>Chromobacterium vaccinii</i> Survives Winter with Support of Bacterial Community |
| title_full | Integrated into Environmental Biofilm <i>Chromobacterium vaccinii</i> Survives Winter with Support of Bacterial Community |
| title_fullStr | Integrated into Environmental Biofilm <i>Chromobacterium vaccinii</i> Survives Winter with Support of Bacterial Community |
| title_full_unstemmed | Integrated into Environmental Biofilm <i>Chromobacterium vaccinii</i> Survives Winter with Support of Bacterial Community |
| title_short | Integrated into Environmental Biofilm <i>Chromobacterium vaccinii</i> Survives Winter with Support of Bacterial Community |
| title_sort | integrated into environmental biofilm i chromobacterium vaccinii i survives winter with support of bacterial community |
| topic | <i>Chromobacterium vaccinii</i> biofilm sharing goods social cheater bog microbiome bacterial genome |
| url | https://www.mdpi.com/2076-2607/8/11/1696 |
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